Embryotoxicity hazard assessment of methylmercury and chromium using embryonic stem cells

Stummann, Tina C.; Hareng, Lars; Bremer, Susanne

doi:10.1016/j.tox.2007.09.022

Cited by 55 publications

(53 citation statements)

References 31 publications

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“…MeHg has been reported to affect the structure of neural microtubules (Stummann et al, 2007;Daré et al, 2001;Castoldi Values are the mean of two experiments performed in triplicate. Vol.…”

Section: Discussionmentioning

confidence: 99%

Characterization of antioxidant protection of cultured neural progenitor cells (NPC) against methylmercury (MeHg) toxicity

et al. 2009

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-Methylmercury (MeHg) is an environmental pollutant known to cause neurobehavioral defects and is especially toxic to the developing brain. With recent studies showing that fetal exposure -bat its effects as well as to clarify the mechanism(s) underlying MeHg toxicity. In the present study, the effects of MeHg on cultured neural progenitor cells (NPC) derived from mouse embryonic brain were -encephalon were more sensitive to MeHg compared to those from the diencephalon. Buthionine sulfoximine (BSO) which is known to inhibit glutathione synthesis accelerated MeHg toxicity. Furthermore, -ic effects. Interestingly, a 12 hr delay in the addition of either antioxidant was still able to prevent the cells and contaminated foods, taking anti-oxidants from foods or supplements may prevent or diminish the toxicological effects of MeHg.

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“…MeHg has been reported to affect the structure of neural microtubules (Stummann et al, 2007;Daré et al, 2001;Castoldi Values are the mean of two experiments performed in triplicate. Vol.…”

Section: Discussionmentioning

confidence: 99%

Characterization of antioxidant protection of cultured neural progenitor cells (NPC) against methylmercury (MeHg) toxicity

et al. 2009

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“…References EST ESAC, 2002;Glenschow et al, 2000Glenschow et al, , 2002Glenschow et al, and 2004 Variations of validated EST with molecular endpoints Bremer et al, 2001;Buesen et al, 2009;Osman et al, 2010;Romero-Lucena., 2010;Seiler et al, 2004;Stummann et al, 2007;van Dartel et al, 2009;2010a, 2010b, 2011a, 2011bACDC Barrier et al, 2010Jeffay et al, 2010Human cell-based embryotoxicity Addler et al, 2008a, 2008b The cellular methods for testing embryotoxicity deserve special mention because, despite the validated in vivo OECD guidelines for testing toxicity to reproduction covering fertility, teratogenicity and development, there are no specific guidelines available for exclusively testing embryotoxicity . Indeed, this is a major gap because a guideline for this purpose would allow the detection of developmental toxins in early development stages without awaiting teratogenicity.…”

Section: Methodsmentioning

confidence: 99%

“…The purpose of using such systems is to correctly classify the substances that were not identified as embryotoxic in the conventional EST. Inclusion of parameters for neuronal differentiation into the EST would allow, for example, classification in accordance with the in vivo data of methylmercury as a highly embryotoxic, whereas the conventional EST failed to correctly classify this compound (Stummann et al, 2007). In order to increase the number of molecular markers as endpoints of embryotoxicity testing, real-time Taqman RT-PCR analyses have been adopted for the EST in pilot studies (zur Nieden et al, 2004).…”

Section: Use Of Biomarkers Of Differentiations As Endpointsmentioning

confidence: 99%

Embryonic Stem Cells in Toxicological Studies

Estevan¹,

Romero²,

Pamies³

et al. 2011

Embryonic Stem Cells - Basic Biology to Bioengineering

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“…An outstanding landmark in the past decade has been the development between stem cell biology and chemistry [1][2][3][4][5][6] . The combination of the two fields will likely provide an impetus for revealing intricate molecular interactions and functions under complex cellular differentiation, which offers systems for drug discovery and sources for potential cell therapy [7][8][9][10] .…”

Section: Introductionmentioning

confidence: 99%

Embryonic stem cell application in drug discovery

Lou¹,

Liang²

2011

Acta Pharmacol Sin

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Embryonic stem (ES) cells and their differentiated progeny offer tremendous potential for regenerative medicine, even in the field of drug discovery. There is an urgent need for clinically relevant assays that make use of ES cells because of their rich biological utility. Attention has been focused on small molecules that allow the precise manipulation of cells in vitro, which could allow researchers to obtain homogeneous cell types for cell-based therapies and discover drugs for stimulating the regeneration of endogenous cells. Such therapeutics can act on target cells or their niches in vivo to promote cell survival, proliferation, differentiation, and homing. In the present paper, we reviewed the use of ES cell models for high-throughput/content drug screening and toxicity assessment. In addition, we examined the role of stem cells in large pharmaceutical companies' R&D and discussed a novel subject, nicheology, in stem cellrelated research fields.

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Embryotoxicity hazard assessment of methylmercury and chromium using embryonic stem cells

Cited by 55 publications

References 31 publications

Characterization of antioxidant protection of cultured neural progenitor cells (NPC) against methylmercury (MeHg) toxicity

Characterization of antioxidant protection of cultured neural progenitor cells (NPC) against methylmercury (MeHg) toxicity

Embryonic Stem Cells in Toxicological Studies

Embryonic stem cell application in drug discovery

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