Emergence and maintenance of multidrug-resistant Escherichia coli of canine origin harbouring a blaCMY-2-IncI1/ST65 plasmid and topoisomerase mutations

Vingopoulou, Elpida I.; Siarkou, Victoria I.; Batzias, Georgios; Kaltsogianni, Flora; Sianou, E.; Tzavaras, Ioannis; Koutinas, A. F.; Saridomichelakis, M. N.; Sofianou, Danai; Tzelepi, E.; Miriagou, Vivì

doi:10.1093/jac/dku090

Cited by 10 publications

(6 citation statements)

References 18 publications

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“…The emergence of this gene, which was pointed out in humans and animals in North and Central America in S. enterica and E. coli isolates (29), was also recently described in dogs, in the United Kingdom, the United States, and Greece (30)(31)(32). However, the proportion reported here (6.5%, ca.…”

Section: Resultssupporting

confidence: 60%

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Haenni¹,

Saras²,

Métayer³

et al. 2014

Antimicrob Agents Chemother

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bIn the community, close contacts between humans and dogs may promote the transfer of extended-spectrum beta-lactamase/ plasmidic AmpC cephalosporinase (ESBL/pAmpC) genes. Large-scale prevalence studies on ESBL/pAmpC carriage in dogs are rare, and data on ESBL/pAmpC plasmids are even more limited. Here, a considerable rate of 18.5% ESBL/pAmpC carriers was found among 368 unrelated healthy dogs in Paris, France. This prevalence is much higher than the one found in healthy humans in the same city (6%) but close to that recently reported in dogs in China (24.5%). All isolates were identified as Escherichia coli, except one Salmonella enterica and one Klebsiella pneumoniae isolate. The sequence type 131 (ST131) clone was rare (2/73 isolates). Interestingly, two plasmids (bla CTX-M-1 /IncI1/ST3 and bla CMY-2 /IncI1/ST2) were unexpectedly highly predominant, raising the question of their successful spread. Considering that CTX-M-1 was recently found to be equally as abundant as CTX-M-15 in healthy Parisian subjects, the question of dogs being a CTX-M-1 reservoir for humans is open. Such a high prevalence of the bla CMY-2 /IncI1/ST2 plasmid may result from the use of cephalexin in veterinary medicine, as previously demonstrated experimentally. In all, our study points out healthy urban dogs as a potential source of ESBL/pAmpC genes that can further disseminate to the human community.

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Section: Resultssupporting

confidence: 60%

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Haenni¹,

Saras²,

Métayer³

et al. 2014

Antimicrob Agents Chemother

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“…This strongly suggests an influence of this mutation for the (fluoro)quinolone resistance in E. coli . Vingopoulou et al [ 75 ] described this ParC E62K substitution previously. They detected this new amino acid exchange in enrofloxacin-resistant E. coli isolated from dog otitis and faecal samples.…”

Section: Resultsmentioning

confidence: 99%

Phenotypic and Genotypic Properties of Fluoroquinolone-Resistant, qnr-Carrying Escherichia coli Isolated from the German Food Chain in 2017

et al. 2021

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Fluoroquinolones are the highest priority, critically important antimicrobial agents. Resistance development can occur via different mechanisms, with plasmid-mediated quinolone resistance (PMQR) being prevalent in the livestock and food area. Especially, qnr genes, commonly located on mobile genetic elements, are major drivers for the spread of resistance determinants against fluoroquinolones. We investigated the prevalence and characteristics of qnr-positive Escherichia (E.) coli obtained from different monitoring programs in Germany in 2017. Furthermore, we aimed to evaluate commonalities of qnr-carrying plasmids in E. coli. We found qnr to be broadly spread over different livestock and food matrices, and to be present in various sequence types. The qnr-positive isolates were predominantly detected within selectively isolated ESBL (extended spectrum beta-lactamase)-producing E. coli, leading to a frequent association with other resistance genes, especially cephalosporin determinants. Furthermore, we found that qnr correlates with the presence of genes involved in resistance development against quaternary ammonium compounds (qac). The detection of additional point mutations in many isolates within the chromosomal QRDR region led to even higher MIC values against fluoroquinolones for the investigated E. coli. All of these attributes should be carefully taken into account in the risk assessment of qnr-carrying E. coli from livestock and food.

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“…Later on, VEB-6 carrying P. mirabilis were isolated from healthy horses (Schultz et al, 2017 ). In Greece, CMY-2 carried on IncI1 plasmid and harbored by ST212 E. coli strains were isolated from diseased canines in 2011 (Vingopoulou et al, 2014 ). More recently, a study conducted in Greek households revealed the detection of extended-spectrum-cephalosporin-resistant E. coli isolates.…”

Section: Distribution Of Esbls and Ampc Producers In Animalsmentioning

confidence: 99%

Prevalence and Emergence of Extended-Spectrum Cephalosporin-, Carbapenem- and Colistin-Resistant Gram Negative Bacteria of Animal Origin in the Mediterranean Basin

et al. 2018

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In recent years, extended ESBL and carbapenemase producing Gram negative bacteria have become widespread in hospitals, community settings and the environment. This has been triggered by the few therapeutic options left when infections with these multi-drug resistant organisms occur. The emergence of resistance to colistin, the last therapeutic option against carbapenem-resistant bacteria, worsened the situation. Recently, animals were regarded as potent antimicrobial reservoir and a possible source of infection to humans. Enteric Gram negative bacteria in animals can be easily transmitted to humans by direct contact or indirectly through the handling and consumption of undercooked/uncooked animal products. In the Mediterranean basin, little is known about the current overall epidemiology of multi-drug resistant bacteria in livestock, companion, and domestic animals. This review describes the current epidemiology of ESBL, carbapenemase producers and colistin resistant bacteria of animal origin in this region of the world. The CTX-M group 1 seems to prevail in animals in this area, followed by SHV-12 and CTX-M group 9. The dissemination of carbapenemase producers and colistin resistance remains low. Isolated multi-drug resistant bacteria were often co-resistant to non-beta-lactam antibiotics, frequently used in veterinary medicine as treatment, growth promoters, prophylaxis and in human medicine for therapeutic purposes. Antibiotics used in veterinary medicine in this area include mainly tetracycline, aminoglycosides, fluoroquinolones, and polymyxins. Indeed, it appears that the emergence of ESBL and carbapenemase producers in animals is not related to the use of beta-lactam antibiotics but is, rather, due to the co-selective pressure applied by the over usage of non-beta-lactams. The level of antibiotic consumption in animals should be, therefore, re-considered in the Mediterranean area especially in North Africa and western Asia where no accurate data are available about the level of antibiotic consumption in animals.

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Emergence and maintenance of multidrug-resistant Escherichia coli of canine origin harbouring a blaCMY-2-IncI1/ST65 plasmid and topoisomerase mutations

Abstract: The long-term maintenance of FQ- and ESC-resistant clones harbouring topoisomerase mutations and a blaCMY-2-IncI1/ST65 plasmid in canine commensal flora after prolonged antimicrobial use may contribute to the dissemination of multidrug resistance.

Cited by 10 publications

References 18 publications

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Phenotypic and Genotypic Properties of Fluoroquinolone-Resistant, qnr-Carrying Escherichia coli Isolated from the German Food Chain in 2017

Prevalence and Emergence of Extended-Spectrum Cephalosporin-, Carbapenem- and Colistin-Resistant Gram Negative Bacteria of Animal Origin in the Mediterranean Basin

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Emergence and maintenance of multidrug-resistant Escherichia coli of canine origin harbouring a blaCMY-2-IncI1/ST65 plasmid and topoisomerase mutations

Abstract: The long-term maintenance of FQ- and ESC-resistant clones harbouring topoisomerase mutations and a blaCMY-2-IncI1/ST65 plasmid in canine commensal flora after prolonged antimicrobial use may contribute to the dissemination of multidrug resistance.

Cited by 10 publications

References 18 publications

High Prevalence of bla CTX-M-1 /IncI1/ST3 and bla CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

High Prevalence of bla CTX-M-1 /IncI1/ST3 and bla CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

Phenotypic and Genotypic Properties of Fluoroquinolone-Resistant, qnr-Carrying Escherichia coli Isolated from the German Food Chain in 2017

Prevalence and Emergence of Extended-Spectrum Cephalosporin-, Carbapenem- and Colistin-Resistant Gram Negative Bacteria of Animal Origin in the Mediterranean Basin

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High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France

High Prevalence of bla _CTX-M-1 /IncI1/ST3 and bla _CMY-2 /IncI1/ST2 Plasmids in Healthy Urban Dogs in France