Emergence of a Nephropathogenic Avian Infectious Bronchitis Virus with a Novel Genotype in India

Bayry, Jagadeesh; Goudar, Mallikarjun S.; Nighot, Prashant K.; Kshirsagar, Supriya G.; Ladman, B. S.; Gelb, Jack; Ghalsasi, Govind R.; Kolte, G. N.

doi:10.1128/jcm.43.4.2039.2005

Cited by 18 publications

(26 citation statements)

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“…In India, IBV has previously been isolated from kidney samples of gout cases (Bayry et al, 2005). In 2011 and 2012, the Indian broiler chicken industry experienced severe outbreaks of gout that caused major financial losses from high mortality with concomitant high cost of treatments.…”

Section: Introductionmentioning

confidence: 99%

Role of chicken astrovirus as a causative agent of gout in commercial broilers in India

Bulbule¹,

Mandakhalikar

Kapgate³

et al. 2013

Avian Pathology

113

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Several outbreaks of gout were reported in commercial broilers in India during 2011 and 2012, causing up to 40% mortality in the birds. Gross and histopathological observations confirmed gout. Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis from kidney samples of gout-affected birds indicated the presence of chicken astrovirus (CAstV) in 41.7% of cases and a mixed infection of CAstV and avian nephritis virus (ANV) in 36.4% of cases. CAstV isolated from gout-affected kidneys by inoculating embryonated specific pathogen free (SPF) eggs showed dwarfing in embryos and a cytopathic effect in chicken embryo kidney cells. Inoculation of 1-day-old SPF and broiler chicks with CAstVs caused gout and mortality between 4 and 10 days post inoculation. Virus isolation and qRT-PCR analysis showed the presence of only CAstV in inoculated chicks. Sequence analysis of capsid genes indicated a major group of Indian CAstVs that displayed 92.0 to 99.2% intergroup amino acid identity and 83.9 to 90.4% identity with subgroup Bi CAstVs of UK origin. We designated this group Indian Bi. Analysis of the partial polymerase amino acid sequences of our isolates indicated two groups of CAstVs (Indian 1 and 2) that displayed 90.2 to 95.5% amino acid identity between them. We thus report for the first time that, in addition to infectious bronchitis virus and ANV, CAstVs are a causative agent of gout.

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Section: Introductionmentioning

confidence: 99%

Role of chicken astrovirus as a causative agent of gout in commercial broilers in India

Bulbule¹,

Mandakhalikar

Kapgate³

et al. 2013

Avian Pathology

113

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“…Results of IBV isolation in ECE revealed that, 10 flocks (2,4,9,12,19,21,25,27,41 and 42) showed variable degrees of curling and dwarfing of the inoculated embryos after 3-5 passages. The recorded results were in agreement with IBV isolation as recorded previously [21,22].…”

Section: Discussionmentioning

confidence: 99%

“…Diversity in S1 gene probably results from nucleotide point mutations, insertions, deletions or RNA recombination of the S1 genes [6,7] or the use of multiple vaccines, which cause immune pressure that might lead to emergence of new variants [8]. Consequently, the evolution of new IBV strains is due to simultaneous infection of multiple virus types and the use of live vaccines makes vaccination is only partially successful due to continual emergence of antigenic variants [9].…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Analysis of Infectious Bronchitis Viruses Currently Circulating in the Egyptian Field

Ghanem

Awad

Hussein

et al. 2019

Zagazig Veterinary Journal

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One of the major problems of infectious bronchitis virus (IBV) is the frequent emergence of new variants in the Egyptian field. In the present study, 42 broiler chicken flocks suffered from respiratory troubles were investigated for infectious bronchitis virus in 10 Governorates in Egypt during 2016-2018. Ten out of 42 examined flocks revealed variable degrees of embryo lesions after 3-5 passages with negative heamagglutination (HA) activity. From these 10 flocks, IBV was confirmed in six flocks using reverse transcriptase polymerase chain reaction (RT-PCR) assay. Four IBV-positive isolates were selected for further sequence analysis. Partial sequencing of S1 gene revealed four IBV variant-2 isolates circulating among chickens in Egypt. These isolates are IB-Beh-Ch-F2-2016, IB-Sh-Ch-F25-2017, IB-Sh-Ch-F41-2018 and IB-Sh-Ch-F42-2018 and submitted on Gen Bank with accession numbers MH460643, MH460644, MK408615 and MK408616, respectively. The IB-Sh-Ch-F25-2017 isolate had only one amino acid substitution while IB-Beh-Ch-F2-2016, IB-Sh-Ch-F41-2018 and IB-Sh-Ch-F42-2018 isolates had much higher genetic diversity. The similarity between classic viruses of vaccine origin used in Egypt (H120, Ma5) and our four filed isolates ranged between 75.7 and 80.4%. It could be concluded that IB variant-2 strains still circulate in the Egyptian field in spite of vaccination. Therefore we need to revise the IBV vaccines used in Egypt and try to prepare local vaccines with periodic evaluation of cross protection of such vaccines.

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“…In the Indian subcontinent, antibodies to several "American" (Muneer et al, 1987a) and "European" (Ahmed et al, 2007) IB variants have been demonstrated in Pakistan, but virological studies have still to be performed. An IBV isolated in India in the early 2000s from cases of nephrosis, was reported to have a unique S1 sequence, indicating it to be different from other known IBVs (Bayry et al, 2005).…”

Section: History Of Infectious Bronchitis Variantsmentioning

confidence: 99%

Infectious bronchitis virus in Asia, Africa, Australia and Latin America: history, current situation and control measures

Wit

Cook²,

Heijden

2010

Rev. Bras. Cienc. Avic.

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Emergence of a Nephropathogenic Avian Infectious Bronchitis Virus with a Novel Genotype in India

Cited by 18 publications

References 0 publications

Role of chicken astrovirus as a causative agent of gout in commercial broilers in India

Role of chicken astrovirus as a causative agent of gout in commercial broilers in India

Phylogenetic Analysis of Infectious Bronchitis Viruses Currently Circulating in the Egyptian Field

Infectious bronchitis virus in Asia, Africa, Australia and Latin America: history, current situation and control measures

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