Emergence of NDM-1-producing Acinetobacter pittii in Brazil

Pagano, Mariana; Poirel, Laurent; Martins, Andreza Francisco; Rozales, Franciéli Pedrotti; Zavascki, Alexandre Prehn; Barth, Afonso Luís; Nordmann, Patrice

doi:10.1016/j.ijantimicag.2014.12.011

Cited by 31 publications

(25 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…NDM-1 producers generally associated with Enterobacteriaceae species have also been reported, albeit with much lower frequency, in Latin American countries, including Guatemala, Mexico, Colombia, and Brazil (3). Moreover, production of NDM-1 in this geographic region has also been noted in Acinetobacter baumannii in Honduras and Brazil (3,4) and in Acinetobacter pittii in Paraguay and Brazil (5,6). Here, we report the first case of an NDM-1-producing Acinetobacter species in Argentina, an Acinetobacter bereziniae clinical isolate.…”

supporting

confidence: 51%

See 1 more Smart Citation

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Brovedan

Marchiaro

Morán-Barrio

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

b The New Delhi metallo-␤-lactamase (NDM-1) was initially identified in clinical isolates of Escherichia coli and Klebsiella pneumoniae in Sweden from a patient previously hospitalized in India (1). Since then, bla NDM-1 has frequently been reported in Enterobacteriaceae and Acinetobacter spp., with a fast dissemination in the Indian subcontinent, the Balkan countries, China, and the Middle East (2). NDM-1 producers generally associated with Enterobacteriaceae species have also been reported, albeit with much lower frequency, in Latin American countries, including Guatemala, Mexico, Colombia, and Brazil (3). Moreover, production of NDM-1 in this geographic region has also been noted in Acinetobacter baumannii in Honduras and Brazil (3,4) and in Acinetobacter pittii in Paraguay and Brazil (5, 6). Here, we report the first case of an NDM-1-producing Acinetobacter species in Argentina, an Acinetobacter bereziniae clinical isolate. We describe also the complete sequence of a bla NDM-1 -containing plasmid in this strain.(Part of this work was presented at the 10th International Symposium on the Biology of Acinetobacter 2015, Athens, Greece, 3 to 6 June 2015 [7].)A. bereziniae HPC229 was isolated on June 2014 from a blood sample of a 53-year-old female patient that underwent chemotherapy due to leukemia at a hospital located in Rosario, Argentina. The patient was treated with ciprofloxacin plus tigecycline, resulting in clinical and microbiological cure as evaluated by negative blood cultures. The patient was readmitted to the hospital 3 months later with symptoms of severe sepsis and died due to septic shock, with positive blood cultures that grew Escherichia coli.HPC229, originally identified as Acinetobacter lwoffii by the Vitek 2 system (bioMérieux), was reclassified by DNA sequence comparison analyses of its 16S rRNA, gyrB (8), and rpoB (9) genes. The highest identity was found to the corresponding orthologs of the A. bereziniae ATCC 17924 type strain (99.9%, 99.7%, and 99.8%, respectively). The antibiotic susceptibility profile of HPC229 was determined by either the Vitek 2 system or the agar dilution method. The interpretation of the obtained MICs based on CLSI breakpoints (10) indicated resistance to ␤-lactams, including carbapenems (Table 1).PCR amplification using specific primers for the bla IMP , bla VIM , bla SPM , bla NDM , bla OXA-23-like , bla OXA-24/40-like , and bla OXA-58-like genes (11-13) and sequencing analysis revealed the presence of bla NDM-1 . Evidence that bla NDM-1 was located in a plasmid was first obtained by plasmid curing (14) in which HPC229 was cultured in 5 ml of LB liquid medium containing 0.2 ml of 10% SDS for 48 h at 40°C. This procedure allowed the isolation of HPC229c, which showed a marked increase in susceptibility to ␤-lactams (Table 1). Susceptibility to aztreonam as judged by the disk diffusion assay, in contrast, showed no differences between HPC229 and HPC229c. All conjugation attempts using HPC229 as the donor and Escherichia coli DH5␣, A. baumannii ATCC 17978, or Pseudo...

show abstract

supporting

confidence: 51%

“…The above findings represent a worrisome concern, especially when considering that Acinetobacter species other than A. baumannii can act as reservoirs of bla NDM genes and most probably contribute to their spreading among clinically relevant Enterobacteriaceae species (6,19).…”

mentioning

confidence: 99%

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Brovedan

Marchiaro

Morán-Barrio

et al. 2015

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…The bla OXA-51-like gene was confirmed, but bla OXA23-like , bla OXA-24-like , bla OXA-58-like and bla OXA-143 genes were absent in this isolate. So far, the only previously identified A. pittii (ST 119) carrying blaNDM-1 gene in Brazilian territory was reported in a colonizing isolate from a 66-year-old man with bladder carcinoma admitted to a tertiary hospital in Porto Alegre, Rio Grande do Sul 10 .…”

Section: Discussionmentioning

confidence: 99%

Earliest identification of New Delhi metallo-β-lactamase 1 (NDM-1) in Acinetobacter pittii in Brazil

Deglmann

Kobs

Oliveira

et al. 2019

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

We report the occurrence in Brazil of the bla NDM-1 gene in Acinetobacter pittii, prior to the previously described first reports regarding the species Providencia rettgeri and Enterobacter hormaechei. Clinical isolates were investigated by polymerase chain reaction followed by bidirectional sequencing, and species was confirmed by 16S rDNA sequencing and matrix-assisted laser desorption-ionization time-of-flight spectrometry. A. pittii carrying bla NDM-1 was confirmed in a patient with no national or international travel history, or transfer from another hospital. The findings warn of the possibility of silent spread of bla NDM-1 to the community.

show abstract

“…1 The New Delhi metallo-β-lactamase (NDM-1) is the most common class B carbapenemase among Enterobacteriaceae, and it has been detected increasingly frequently in several countries, 2 including Brazil. [3][4][5][6][7][8] The aim of this study was to evaluate the characteristics of transformants obtained from NDM-1-production in different bacterial species of Enterobacteriaceae identified in southern Brazil.…”

Section: Characterization Of Transformants Obtained From Ndm-1-producmentioning

confidence: 99%

Characterization of Transformants Obtained From NDM-1–Producing Enterobacteriaceae in Brazil

Rozales

Magagnin

Campos

et al. 2017

Infect. Control Hosp. Epidemiol.

Self Cite

View full text Add to dashboard Cite

Emergence of NDM-1-producing Acinetobacter pittii in Brazil

Cited by 31 publications

References 5 publications

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Earliest identification of New Delhi metallo-β-lactamase 1 (NDM-1) in Acinetobacter pittii in Brazil

Characterization of Transformants Obtained From NDM-1–Producing Enterobacteriaceae in Brazil

Contact Info

Product

Resources

About

Emergence of NDM-1-producing Acinetobacter pittii in Brazil

Cited by 31 publications

References 5 publications

Complete Sequence of a bla NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Complete Sequence of a bla NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Earliest identification of New Delhi metallo-β-lactamase 1 (NDM-1) in Acinetobacter pittii in Brazil

Characterization of Transformants Obtained From NDM-1–Producing Enterobacteriaceae in Brazil

Contact Info

Product

Resources

About

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina

Complete Sequence of a bla _NDM-1 -Harboring Plasmid in an Acinetobacter bereziniae Clinical Strain Isolated in Argentina