2022
DOI: 10.1128/aem.02307-21
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Emergence of Phenotypically Distinct Subpopulations Is a Factor in Adaptation of Recombinant Saccharomyces cerevisiae under Glucose-Limited Conditions

Abstract: The yeast Saccharomyces cerevisiae is an attractive microbial host for industrial production and is used widely for manufacturing, e.g., pharmaceuticals. Chemostat cultivation mode is an efficient cultivation strategy for industrial production processes as it ensures a constant, well-controlled cultivation environment.

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Cited by 7 publications
(5 citation statements)
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“…During cell cultivation, different isogenic subpopulations (i.e., subpopulations with the same genetic background) tend to form [ 13 ]. These subpopulations often show different physiological traits, such as different stress responses, growth, and/or productivity [ 48 50 ]. Understanding how subpopulations emerge is crucial for optimising protocols and tailoring yeast strains for targeted industrial purposes, ultimately enhancing yields and efficiency.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…During cell cultivation, different isogenic subpopulations (i.e., subpopulations with the same genetic background) tend to form [ 13 ]. These subpopulations often show different physiological traits, such as different stress responses, growth, and/or productivity [ 48 50 ]. Understanding how subpopulations emerge is crucial for optimising protocols and tailoring yeast strains for targeted industrial purposes, ultimately enhancing yields and efficiency.…”
Section: Resultsmentioning
confidence: 99%
“…For example, an early study showed that during L-valine production, only a minor part of the bulk population is actively generating L-valine, while the majority is producing biomass [ 52 ]. In insulin-producing yeast, three different subpopulations have been found under glucose-limited conditions, probably to increase fitness [ 50 ]. This phenomenon of distributing different phenotypes within a population is often referred to as bet-hedging, aimed to increase survivability in face of unexpected events [ 53 ].…”
Section: Resultsmentioning
confidence: 99%
“…Optimal bioprocess productivity requires a stable and predictable microbial performance. Population heterogeneity lowers the predictability of a bioprocess and may affect its performance due to the emergence of a low-performing subpopulation [19,49]. Changes in bioprocess conditions that may have a high impact on a microbial cell factory can be easily identi ed by investigating the relationship between either R(t) or R(p) and the respective performance of a function (Fig.…”
Section: Discussionmentioning
confidence: 99%
“…In populations formed by diverse cell types, from microbes to human cells, pre-existing variation or de novo mutation and selection can lead to subpopulations with different phenotypes ( 1 3 ). One important example of co-existing sub-populations is found in chronic Pseudomonas aeruginosa infections.…”
Section: Introductionmentioning
confidence: 99%