Emergence of Raoultella ornithinolytica Coproducing IMP-4 and KPC-2 Carbapenemases in China

Zheng, Beiwen; Zhang, Jing; Ji, Jinru; Fang, Yanran; Shen, Ping; Chen, Ying; Lv, Jifang; Xiao, Yonghong; Li, Lanjuan

doi:10.1128/aac.01363-15

Cited by 56 publications

(62 citation statements)

References 24 publications

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“…B-lactamase genes had been identified on a couple of Raoultella spp. big plasmids and megaplasmids before (bla IMP-4 and bla KPC-2 on 450 kb plasmid, bla NDM-1 on 41 kb and 100 kb plasmids, bla OXA-48 on 63 kb or bla KPC-2 40 kb plasmid) (Sun et al 2015;Zheng et al 2015;Yao et al 2017). However, this is the first observation of a bla gene on a very small plasmid in Raoultella spp.…”

Section: Discussionmentioning

confidence: 64%

Diversity of β-lactam resistance genes in gram-negative rods isolated from a municipal wastewater treatment plant

2019

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Urban wastewater treatment plants (UWTPs) are considered hot spots for the accumulation and transfer of antibiotic resistant bacteria and their genes. We investigated the prevalence, diversity, and genomic localization of β-lactamase resistance genes in wastewater from different parts of an UWTP, such as raw wastewater, wastewater from biological reactor, and treated wastewater, collected from the UWTP of Warsaw, Poland. In this study, we focused on gram-negative rods, mainly Enterobacteriaceae, and used Multiplex PCR to identify various β-lactamase resistance genes (bla). Susceptibility to a number of antibiotics and genomic localization of β-lactamase resistance genes were then determined using disc-diffusion and Southern hybridization methods, respectively. No differences in the frequency of different types of bla genes between the sampling points were discerned. Three new variants of β-lactamase genes (bla CMY-157 , bla MOX-13 , and bla FOX-15 ) were identified. Four bla genes (bla TEM-12 , bla TEM-30 , bla TEM-47/68 , bla ACT ) that had never been found in UWTPs were identified in this study. Nine of the identified bla genes had been found in the same environment previously. The bla FOX-15 variant on a Kluyvera sp. plasmid and bla GES type on Raoultella spp. plasmids were observed for the first time in these genera. There was a decrease in the number of multidrug resistant strains in the effluent compared to the influent. Finally, a significantly higher number of cefotaxime and carbapenem unsusceptible strains were detected in the influent than in the effluent. The results strongly support the hypothesis of UWTPs as a hot spot for antibiotic resistant genes (ARGs) and antibiotic resistant bacteria (ARB) accumulation and indicate that β-lactamase genes are widely disseminated among gram-negative rods isolated from this environment.

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Section: Discussionmentioning

confidence: 64%

Diversity of β-lactam resistance genes in gram-negative rods isolated from a municipal wastewater treatment plant

2019

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“…[52][53][54][55] Nonetheless, a few strains of R. ornithinolytica generating simultaneously different types of beta-lactamase (TEM, SHV, KPC and OXA) were described. 42,56 The first case of co-existence of bla KPC-2 and bla IMP-4 carbapenemase genes in the genus Raoultella was reported by Zheng et al (2015) in a 13-year-old male patient with postoperative infection after an orthopedic surgery. 57 The R. ornithinolytica strain was identified in this report by 16S rRNA sequencing and antimicrobial susceptibility was determined with VITEK ® 2 and Etest strips (Bioméreux, France) on 7 samples collected from the wound fluid and necrotic tissue.…”

Section: Virulence and Resistancementioning

confidence: 99%

“…42,56 The first case of co-existence of bla KPC-2 and bla IMP-4 carbapenemase genes in the genus Raoultella was reported by Zheng et al (2015) in a 13-year-old male patient with postoperative infection after an orthopedic surgery. 57 The R. ornithinolytica strain was identified in this report by 16S rRNA sequencing and antimicrobial susceptibility was determined with VITEK ® 2 and Etest strips (Bioméreux, France) on 7 samples collected from the wound fluid and necrotic tissue. 57 The isolated strains were reported as resistant to all antimicrobials except ciprofloxacin.…”

Section: Virulence and Resistancementioning

confidence: 99%

<p><em>Raoultella ornithinolytica</em>: Emergence and Resistance</p>

Hajjar¹,

Ambaraghassi²,

Sebajang³

et al. 2020

IDR

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Raoultella ornithinolytica is an encapsulated Gram-negative, oxidase-negative, catalase-positive, aerobic, non-motile rod that belongs to the Enterobacteriaceae family. This bacterium was initially classified in the genus Klebsiella as Klebsiella ornithinolytica, until the creation of the genus Raoultella in 2001. R. ornithinolytica is usually found in water environments and soil, and due to its ability to convert histidine to histamine, it has been associated with histamine poisoning in humans. R. ornithinolytica is an emerging entity in human infections, with several reports of virulent infections in comorbid at-risk patients. Increasing reports are potentially due to better and more precise identification tools. The objective of this article is to provide a comprehensive review of reported cases of R. ornithinolytica infections, the emergent virulence of described multiresistant strains, and an overview of currently used identification methods.

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“…10 Enterobacteriaceae species produced bla IMP-4 , but E. cloacae was the most frequent (60.4%, 29/48) and possessed bla IMP-4 on conjugatable HI2 (22/29 or L/M (7/29) plasmids demonstrating a plausible mechanism for bla IMP-4 proliferation throughout the region [Sidjabat 2015]. A pediatric patient in China in June 2014 had seven closely related bla IMP-4 producing Raoultella ornithinolytica isolated from an infected surgical wound, including the first observation of an R. ornithinolytica in China producing bla IMP-4 and bla KPC-2 [Zheng 2015]. Analysis of mobile genetic elements surrounding MBLs in Enterobacteriaceae collected in Spain from February to July 2009 found that all 14 bla VIM-1 genes were associated with class 1 integrons but a mixture of genetic backgrounds (2/14 bla VIM-1 , IncU and 12/14 unidentified plasmid type) [Zamorano 2015].…”

Section: Impmentioning

confidence: 99%

The rapid spread of carbapenem-resistant Enterobacteriaceae

Potter

D’Souza

Dantas

2016

Drug Resistance Updates

335

211

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Carbapenems, our one-time silver bullet for multidrug resistant bacterial infections, are now threatened by widespread dissemination of carbapenem-resistant Enterobacteriaceae (CRE). Successful expansion of Enterobacteriaceae clonal groups and frequent horizontal gene transfer of carbapenemase expressing plasmids are causing increasing carbapenem resistance. Recent advances in genetic and phenotypic detection facilitate global surveillance of CRE diversity and prevalence. In particular, whole genome sequencing enabled efficient tracking, annotation, and study of genetic elements colocalized with carbapenemase genes on chromosomes and on plasmids. Improved characterization helps detail the co-occurrence of other antibiotic resistance genes in CRE isolates and helps identify pan-drug resistance mechanisms. The novel β-lactamase inhibitor, avibactam, combined with ceftazidime or aztreonam, is a promising CRE treatment compared to current colistin or tigecycline regimens. To halt increasing CRE-associated morbidity and mortality, we must continue quality, cooperative monitoring and urgently investigate novel treatments.

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Emergence of Raoultella ornithinolytica Coproducing IMP-4 and KPC-2 Carbapenemases in China

Cited by 56 publications

References 24 publications

Diversity of β-lactam resistance genes in gram-negative rods isolated from a municipal wastewater treatment plant

Diversity of β-lactam resistance genes in gram-negative rods isolated from a municipal wastewater treatment plant

<p><em>Raoultella ornithinolytica</em>: Emergence and Resistance</p>

The rapid spread of carbapenem-resistant Enterobacteriaceae

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