Emergence of Sequence Type 398 as a Community- and Healthcare-Associated Methicillin-Susceptible Staphylococcus aureus in Northern Manhattan

Uhlemann, Anne‐Catrin; Hafer, Cory; Miko, Benjamin A.; Sowash, Madeleine; Sullivan, Sean B.; Shu, Qiuhu; Lowy, Franklin D.

doi:10.1093/cid/cit375

Cited by 40 publications

(61 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1; see also Table S1 in the supplemental material). These isolates were selected from community-based studies on the transmission of S. aureus in Northern Manhattan (NM) and the Bronx (22, 24, 28, 33) and from t571-MSSA-colonized veterinarians and pigs in Minnesota. To increase the genetic, temporal, and geographic diversity in the sample set for comparative sequence analyses, we also included previously published ST398 MSSA and MRSA sequences from international HA- and LA-CC398 collections (31).…”

Section: Resultsmentioning

confidence: 99%

“…2B). Importantly, none of the previous ST398 isolates from hospital-associated infections (28), which had been thought to represent a potential nosocomial ST398 outbreak, were closely related.…”

Section: Resultsmentioning

confidence: 99%

“…Individuals who had previously tested positive for ST398 colonization or infection were eligible to enroll (28, 42). These index participants were asked to complete a structured questionnaire and provide information on their daily contacts and the nature and frequency of these interactions.…”

Section: Methodsmentioning

confidence: 99%

“…We also selected an additional 135 ST398 isolates from previous studies on S. aureus infection and colonization in Northern Manhattan and the Bronx as follows: (i) MSSA infections between 2007 and 2011 ( n = 69) (24, 28), (ii) infectious isolates from the Dominican Republic and Martinique ( n = 14) (22), (iii) community studies on colonization between 2003 and 2010 ( n = 38) (23, 33, 42), and (iv) colonizing isolates from pigs in Minnesota and swine veterinarians in Minnesota, Illinois, and Indiana ( n = 14) (26). In total, we obtained 288 novel sequences.…”

Section: Methodsmentioning

confidence: 99%

“…This lineage has been notably absent from other regions such as large parts of the United States (11), although it has sporadically been reported in pigs and veterinarians in the Midwest (26). ST398 MSSA strains are encountered as both CA and HA pathogens (27, 28). Remarkably, a number of studies from such diverse settings as community households, a jail holding tank, and an intensive care unit have suggested that ST398 MSSA might be uniquely transmissible between humans (24, 27, 29).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Evolutionary Dynamics of Pandemic Methicillin-Sensitive Staphylococcus aureus ST398 and Its International Spread via Routes of Human Migration

Uhlemann

McAdam

Sullivan

et al. 2017

mBio

View full text Add to dashboard Cite

Methicillin-susceptible Staphylococcus aureus (MSSA) accounts for the majority of S. aureus infections globally, and yet surprisingly little is known about its clonal evolution. We applied comparative whole-genome sequencing (WGS) analyses to epidemiologically and geographically diverse ST398-MSSA, a pandemic lineage affecting both humans and livestock. Bayesian phylogenetic analysis predicted divergence of human-associated ST398-MSSA ~40 years ago. Isolates from Midwestern pigs and veterinarians differed substantially from those in New York City (NYC). Pig ST398 strains contained a large region of recombination representing imports from multiple sequence types (STs). Phylogeographic analyses supported the spread of ST398-MSSA along local cultural and migratory links between parts of the Caribbean, North America, and France, respectively. Applying pairwise single-nucleotide polymorphism (SNP) distances as a measure of genetic relatedness between isolates, we observed that ST398 not only clustered in households but also frequently extended across local social networks. Isolates collected from environmental surfaces reflected the full diversity of colonizing individuals, highlighting their potentially critical role as reservoirs for transmission and diversification. Strikingly, we observed high within-host SNP variability compared to our previous studies on the dominant methicillin-resistant Staphylococcus aureus (MRSA) clone USA300. Our data indicate that the dynamics of colonization, persistence, and transmission differ substantially between USA300-MRSA and ST398-MSSA. Taken together, our study reveals local and international routes of transmission for a major MSSA clone, indicating key impacts of recombination and mutation on genetic diversification and highlighting important ecological differences from epidemic USA300. Our study demonstrates extensive local and international routes of transmission for a major MSSA clone despite the lack of substantial antibiotic resistance.

show abstract