Emerging alternatives against Clostridioides difficile infection

Romero-Rodríguez, A.; Peña, C. Martínez de la; Troncoso-Cotal, S.; Guzmán, C.; Sánchez, Sergio

doi:10.1016/j.anaerobe.2022.102638

Cited by 4 publications

(1 citation statement)

References 101 publications

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“… Clostridioides difficile infection (CDI) is a major concern in health care settings and is one of the leading causes of severe antibiotic-induced diarrhea worldwide. Accurate diagnosis of CDI is crucial, especially in the context of nosocomial surveillance, as the incidences of CDI-related morbidity, mortality, and even drug resistance in hospitalized patients have increased exponentially over the last 2 decades ( 29 ). Currently, the main pathogenic toxin factors of C. difficile are toxin A (TcdA), an enterotoxin, and toxin B (TcdB), a cytotoxin, which can cause diseases ranging from mild diarrhea to pseudomembranous colitis ( 30 , 31 ).…”

Section: Discussionmentioning

confidence: 99%

Establishment of a Novel Detection Platform for Clostridioides difficile Toxin Genes Based on Orthogonal CRISPR

Jiang

Shen

2023

Microbiol Spectr

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C. difficile is currently the primary causative agent of hospital-acquired antibiotic-induced diarrhea, and timely and accurate diagnosis is crucial for hospital-acquired infection control and epidemiological investigation. Here, a new method for the identification of C. difficile was developed based on the recently popular CRISPR technology, and an orthogonal CRISPR dual system was utilized for the simultaneous detection of toxin genes A and B.

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Section: Discussionmentioning

confidence: 99%

Establishment of a Novel Detection Platform for Clostridioides difficile Toxin Genes Based on Orthogonal CRISPR

Jiang

Shen

2023

Microbiol Spectr

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Clostridioides difficile resistance to antibiotics, including post-COVID-19 data

Boyanova,

Dimitrov,

Gergova

et al. 2023

Expert Review of Clinical Pharmacology

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Host origin of microbiota drives functional recovery andClostridioides difficileclearance in mice

Millard,

Vendrov,

Young

et al. 2024

Preprint

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Colonization resistance provided by the gut microbiota is essential for resisting both initial Clostridioides difficile infection (CDI) and potential recurrent infection (rCDI). Although fecal microbiota transplantation (FMT) has been successful in treating rCDI by restoring microbial composition and function, mechanisms underlying efficacy of standardized stool-derived products remain poorly understood. Using a combination of 16S rRNA gene-based and metagenomic sequencing alongside metabolomics, we investigated microbiome recovery following FMT from human and murine donor sources in a mouse model of rCDI. We found that a human-derived microbiota was less effective in clearing C. difficile compared to a mouse-derived microbiota, despite successful microbial engraftment and recovery of bacterial functional potential. Metabolomic analysis revealed deficits in secondary metabolites, suggesting a functional remodeling between human microbes in their new host environment. Collectively, our data revealed additional environmental, ecological, or host factors involved in FMT-based recovery from rCDI.

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Emerging alternatives against Clostridioides difficile infection

Cited by 4 publications

References 101 publications

Establishment of a Novel Detection Platform for Clostridioides difficile Toxin Genes Based on Orthogonal CRISPR

Establishment of a Novel Detection Platform for Clostridioides difficile Toxin Genes Based on Orthogonal CRISPR

Clostridioides difficile resistance to antibiotics, including post-COVID-19 data

Host origin of microbiota drives functional recovery andClostridioides difficileclearance in mice

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