Emerging artificial metalloenzymes for asymmetric hydrogenation reactions

Goralski, Sean T.; Rose, Michael J.

doi:10.1016/j.cbpa.2021.102096

Cited by 4 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared with semi-synthetic [Fe] hydrogenase, the catalytic activity of semi-synthetic [Mn] hydrogenase is higher. But it should be noted that [Mn] hydrogenase had a stronger forward reaction tendency [50]. Because Mn I mimics are more stable than Fe II mimics, the synthesis of catalytic [Mn] hydrogenase paved the way for structure-activity studies.…”

Section: Metal Clusters Of Artificial [Fe] Hydrogenasesmentioning

confidence: 99%

Artificial Small Molecules as Cofactors and Biomacromolecular Building Blocks in Synthetic Biology: Design, Synthesis, Applications, and Challenges

Dong

et al. 2023

Molecules

View full text Add to dashboard Cite

Enzymes are essential catalysts for various chemical reactions in biological systems and often rely on metal ions or cofactors to stabilize their structure or perform functions. Improving enzyme performance has always been an important direction of protein engineering. In recent years, various artificial small molecules have been successfully used in enzyme engineering. The types of enzymatic reactions and metabolic pathways in cells can be expanded by the incorporation of these artificial small molecules either as cofactors or as building blocks of proteins and nucleic acids, which greatly promotes the development and application of biotechnology. In this review, we summarized research on artificial small molecules including biological metal cluster mimics, coenzyme analogs (mNADs), designer cofactors, non-natural nucleotides (XNAs), and non-natural amino acids (nnAAs), focusing on their design, synthesis, and applications as well as the current challenges in synthetic biology.

show abstract

Section: Metal Clusters Of Artificial [Fe] Hydrogenasesmentioning

confidence: 99%

Artificial Small Molecules as Cofactors and Biomacromolecular Building Blocks in Synthetic Biology: Design, Synthesis, Applications, and Challenges

Dong

et al. 2023

Molecules

View full text Add to dashboard Cite

show abstract

“…Using oxygen in aerobic metabolism under mild conditions is very attractive since O 2 in the triplet ground state is very stable. Miraculously, metalloenzymes have provided an efficient and alternative solution to this obstacle under mild conditions [6][7][8]. As important metalloenzymes, laccases with copper as active sites catalyzed the oxidation of diverse biologicall and environmental substrates with oxygen [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Fungus-Based MnO/Porous Carbon Nanohybrid as Efficient Laccase Mimic for Oxygen Reduction Catalysis and Hydroquinone Detection

Zhang

2022

Nanomaterials

View full text Add to dashboard Cite

Developing efficient laccase-mimicking nanozymes via a facile and sustainable strategy is intriguing in environmental sensing and fuel cells. In our work, a MnO/porous carbon (MnO/PC) nanohybrid based on fungus was synthesized via a facile carbonization route. The nanohybrid was found to possess excellent laccase-mimicking activity using 2,2′-azinobis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) as the substrate. Compared with the natural laccase and reported nanozymes, the MnO/PC nanozyme had much lower Km value. Furthermore, the electrochemical results show that the MnO/PC nanozyme had high electrocatalytic activity toward the oxygen reduction reaction (ORR) when it was modified on the electrode. The hybrid nanozyme could catalyze the four-electron ORR, similar to natural laccase. Moreover, hydroquinone (HQ) induced the reduction of oxABTS and caused the green color to fade, which provided colorimetric detection of HQ. A desirable linear relationship (0–50 μM) and detection limit (0.5 μM) were obtained. Our work opens a simple and sustainable avenue to develop a carbon–metal hybrid nanozyme in environment and energy applications.

show abstract

Characterization of the interaction of metal-protoporphyrins photosensitizers with β- lactoglobulin

Castillo

Mancillas²,

Hughes³

et al. 2023

Biophysical Chemistry

View full text Add to dashboard Cite

Emerging artificial metalloenzymes for asymmetric hydrogenation reactions

Cited by 4 publications

References 44 publications

Artificial Small Molecules as Cofactors and Biomacromolecular Building Blocks in Synthetic Biology: Design, Synthesis, Applications, and Challenges

Artificial Small Molecules as Cofactors and Biomacromolecular Building Blocks in Synthetic Biology: Design, Synthesis, Applications, and Challenges

Fungus-Based MnO/Porous Carbon Nanohybrid as Efficient Laccase Mimic for Oxygen Reduction Catalysis and Hydroquinone Detection

Characterization of the interaction of metal-protoporphyrins photosensitizers with β- lactoglobulin

Contact Info

Product

Resources

About