Emerging Methods to Generate Artificial Germ Cells from Stem Cells1

Zeng, Fanhui; Huang, Fajun; Guo, Jingjing; Xing-chang, HU; Liu, Changbai; Wang, Hu

doi:10.1095/biolreprod.114.124800

Cited by 18 publications

(15 citation statements)

References 156 publications

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“…Consequently, the combination of niche reprogramming and newly found 3D microenvironment may be useful for stem cell-based artificial germ cell generation. 32…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, the combination of niche reprogramming and newly found 3D microenvironment may be useful for stem cell-based artificial germ cell generation. 32 Although various efforts have been made for the translation of research experiences on tissue engineering especially stem cell differentiation into germ cell, 20,33 proliferation and differentiation of stem cells in 2D culture has remained highly challenging because of technical demands encountered with acquisition of appropriate stem cells and also preparing suitable scaffold supporting stem cell differentiation. Besides biocompatibility, a scaffold should match biomechanical characteristics of the cells and be able to properly support cells during proliferation and differentiation.…”

Section: Discussionmentioning

confidence: 99%

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Three-dimensional wet-electrospun poly(lactic acid)/multi-wall carbon nanotubes scaffold induces differentiation of human menstrual blood-derived stem cells into germ-like cells

et al. 2017

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Infertility caused by the disruption or absence of germ cells is a major and largely incurable medical problem. Germ cells (i.e., sperm or egg) play a key role in the transmission of genetic and epigenetic information across generations. Generation of gametes derived in vitro from stem cells hold promising prospects which could potentially help infertile men and women. Menstrual blood-derived stem cells are a unique stem cell source. Evidence suggests that menstrual blood-derived stem cells exhibit a multi-lineage potential and have attracted extensive attention in regenerative medicine. To maintain the three-dimensional structure of natural extra cellular matrices in vitro, scaffolds can do this favor and mimic a microenvironment for cell proliferation and differentiation. According to previous studies, poly(lactic acid) and multi-wall carbon nanotubes have been introduced as novel and promising biomaterials for the proliferation and differentiation of stem cells. Some cell types have been successfully grown on a matrix containing carbon nanotubes in tissue engineering but there is no report for this material to support stem cells differentiation into germ cells lineage. This study designed a 3D wet-electrospun poly(lactic acid) and poly(lactic acid)/multi-wall carbon nanotubes composite scaffold to compare infiltration, proliferation, and differentiation potential of menstrual blood-derived stem cells toward germ cell lineage with 2D culture. Our primary data revealed that the fabricated scaffold has mechanical and biological suitable qualities for supporting and attachments of stem cells. The differentiated menstrual blood-derived stem cells tracking in scaffolds using scanning electron microscopy confirmed cell attachment, aggregation, and distribution on the porous scaffold. Based on the differentiation assay by RT-PCR analysis, stem cells and germ-like cells markers were expressed in 3D groups as well as 2D one. It seems that poly(lactic acid)/multi-wall carbon nanotubes scaffold-seeded menstrual blood-derived stem cells could be viewed as a novel, safe, and accessible construct for these cells, as they enhance germ-like generation from menstrual blood-derived stem cells.

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“…Consequently, the combination of niche reprogramming and newly found 3D microenvironment may be useful for stem cell-based artificial germ cell generation. 32…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Three-dimensional wet-electrospun poly(lactic acid)/multi-wall carbon nanotubes scaffold induces differentiation of human menstrual blood-derived stem cells into germ-like cells

et al. 2017

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“…Accordingly, CPP have demonstrated utility as valuable vehicle for intracellular delivery of macromolecules, cargo has to be covalently [13] or non-covalently [14] linked to CPPs for efficient uptake. In recent years, numerous applications for CPPs not only have been shown from a vast number of fundamental researches (including CPP-based somatic cell reprogramming [5, 15–18], CPP-mediated genome editing [5, 19–21]), but also a little number of CPP-coupled molecules has entered into a phase II clinical trials [6]. …”

Section: Introductionmentioning

confidence: 99%

Efficient therapeutic delivery by a novel cell-permeant peptide derived from KDM4A protein for antitumor and antifibrosis

Wang

Yang

et al. 2016

Oncotarget

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Cell-penetrating peptide (CPP) based delivery have provided immense potential for the therapeutic applications, however, most of nonhuman originated CPPs carry the risk of possible cytotoxicity and immunogenicity, thus may restricting to be used. Here, we describe a novel human-derived CPP, denoted hPP10, and hPP10 has cell-penetrating properties evaluated by CellPPD web server, as well as In-Vitro and In-Vivo analysis. In vitro studies showed that hPP10-FITC was able to penetrate into various cells including primary cultured cells, likely through an endocytosis pathway. And functionalized macromolecules, such as green fluorescent protein (GFP), tumor-specific apoptosis inducer Apoptin as well as biological active enzyme GCLC (Glutamate-cysteine ligase, catalytic subunit) can be delivered by hPP10 in vitro and in vivo. Collectively, our results suggest that hPP10 provide a novel and versatile tool to deliver exogenous proteins or drugs for clinical applications as well as reprogrammed cell-based therapy.

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“…Promising therapeutic bio-macromolecules such as nucleic acids (DNA and/or RNA) have become widely recognized in the field of reprogramming-based regenerative medicine [1][2][3] and gene editing [4] for drug development research. However, semi-permeable cellular membranes serve as a barrier, which makes it challenging for nucleic acids to enter the cytoplasm because of their size and overall hydrophilic nature [4].…”

Section: Introductionmentioning

confidence: 99%

Intracellular Delivery of DNA and Protein by a Novel Cell-Permeable Peptide Derived from DOT1L

et al. 2020

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Cellular uptake and intracellular release efficiency of biomacromolecules is low because of hurdles in the cell membrane that result in limited access to intra-cellular targets with few functional effects. Cell-penetrating peptides (CPPs) act as cargo delivery vehicles to promote therapeutic molecule translocation. Here, we describe the novel CPP-Dot1l that not only penetrates by itself, but also mediates cargo translocation in cultured cells, as confirmed by fluorescence microscopy and fluorescence spectrophotometry. We conducted cytotoxicity assays and safety evaluations, and determined peptide-membrane interactions to understand the possible pathway for cargo translocation. Additional nucleic acid and covalently conjugated green fluorescence protein (GFP) studies mediated by CPP-Dot1l were conducted to show functional delivery potential. Results indicate that CPP-Dot1l is a novel and effective CPP due to its good penetrating properties in different cell lines and its ability to enter cells in a concentration-dependent manner. Its penetration efficiency can be prompted by DMSO pretreatment. In addition, not only can it mediate plasmid delivery, but CPP-Dot1l can also deliver GFP protein into cytosol. In conclusion, the findings of this study showed CPP-Dot1l is an attractive pharmaceutical and biochemical tool for future drug, regenerative medicine, cell therapy, gene therapy, and gene editing-based therapy development.

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Emerging Methods to Generate Artificial Germ Cells from Stem Cells1

Cited by 18 publications

References 156 publications

Three-dimensional wet-electrospun poly(lactic acid)/multi-wall carbon nanotubes scaffold induces differentiation of human menstrual blood-derived stem cells into germ-like cells

Three-dimensional wet-electrospun poly(lactic acid)/multi-wall carbon nanotubes scaffold induces differentiation of human menstrual blood-derived stem cells into germ-like cells

Efficient therapeutic delivery by a novel cell-permeant peptide derived from KDM4A protein for antitumor and antifibrosis

Intracellular Delivery of DNA and Protein by a Novel Cell-Permeable Peptide Derived from DOT1L

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