Emerging multidrug resistant Mycobacterium tuberculosis strains of the Beijing genotype circulating in Russia express a pattern of biological properties associated with enhanced virulence

Lassounskaia, Elena; Ribeiro, Simone C. M.; Manicheva, Olga; Gomes, Lia Lima; Suffys, Philip Noël; Mokrousov, Igor; Ferrazoli, Lucilaine; Andrade, Marcelle R. M. de; Kritski, Afrânio Lineu; Otten, Tatiana; Kipnis, Thereza Liberman; Silva, Wilmar D. da; Vishnevsky, Boris; Oliveira, Martha Maria de; Gomes, Harrison Magdinier; Baptista, Ida F.; Narvskaya, Olga

doi:10.1016/j.micinf.2010.02.008

Cited by 69 publications

(68 citation statements)

References 21 publications

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“…Mycobacterium tuberculosis strains of the Beijing genotype were collected over a 6-year period (2001 to 2007) from native TB patients attending primary health care clinics in Rio de Janeiro and Sao Paulo in Brazil (19) and in Maputo and the Maputo province in Mozambique (20) as a part of molecular epidemiology studies in these countries. The Russian M. tuberculosis isolate 1471 was isolated from a patient with pulmonary TB in the St. Petersburg Research Institute of Phthisiopulmonology, Russia, and kindly provided by B. Vishnevsky.…”

Section: Mycobacterial Isolates and Genotype Classificationmentioning

confidence: 99%

Mycobacterium tuberculosis Strains of the Modern Sublineage of the Beijing Family Are More Likely To Display Increased Virulence than Strains of the Ancient Sublineage

Gomes²,

et al. 2014

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dStrains of the Beijing genotype family of Mycobacterium tuberculosis are a cause of particular concern because of their increasing dissemination in the world and their association with drug resistance. Phylogenetically, this family includes distinct ancient and modern sublineages. The modern strains, contrary to the ancestral counterparts, demonstrated increasing prevalence in many world regions that suggest an enhanced bacterial pathogenicity. We therefore evaluated virulence of modern versus ancient Beijing strains with similar epidemiological and genotype characteristics. For this, we selected six strains that had very similar 24-locus mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing profiles and belonged to the region of difference 181 (RD181) subgroup but differed using markers (mutT2 and mutT4 genes and NTF locus) that discriminate between modern and ancient Beijing sublineages. The strains were isolated from native patients in Brazil and Mozambique, countries with a low prevalence of Beijing strains. The virulence levels of these strains were determined in models of pulmonary infection in mice and in vitro macrophage infection and compared with that of a strain from Russia, part of the epidemic and hypervirulent Beijing clone B0/W148, and of the laboratory strain H37Rv. The results showed that two of the three modern Beijing strains were highly pathogenic, exhibiting levels of virulence comparable with that of the epidemic Russian strain. In contrast, all isolates of the ancient sublineage displayed intermediate or low virulence. The data obtained demonstrate that the strains of the modern Beijing sublineage are more likely to exhibit highly virulent phenotypes than ancient strains and suggest that genetic alterations characteristic of the modern Beijing sublineage favor selection of highly virulent bacteria. Despite extensive surveillance, tuberculosis (TB) remains a serious public health problem. In different parts of the world, there is concern about TB caused by the East Asian/Beijing lineage of Mycobacterium tuberculosis, demonstrating increasing prevalence in the global M. tuberculosis population (1). Clinical and epidemiological studies demonstrated that emergence of the Beijing strains could be associated with high levels of bacterial resistance to multiple drugs (2, 3) and enhanced pathogenicity of these strains, leading to increased transmissibility (4) and rapid progression from infection to active disease (5). However, the data on evaluation of the virulence of Beijing isolates were inconclusive, demonstrating a wide range of inflammatory and virulence phenotypes, as determined in animal models (6,7,8) and in vitro models of macrophage infection (9, 10).Such differences in the virulence of Beijing strains could be associated with genetic heterogeneity of the Beijing M. tuberculosis lineage. Indeed, bacterial genotyping and sequencing demonstrated that the Beijing lineage, having in common a characteristic spoligotype signature and lack of the region ...

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Section: Mycobacterial Isolates and Genotype Classificationmentioning

confidence: 99%

Mycobacterium tuberculosis Strains of the Modern Sublineage of the Beijing Family Are More Likely To Display Increased Virulence than Strains of the Ancient Sublineage

Gomes²,

et al. 2014

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“…Retrospective collections of M. tuberculosis DNA used in this study were partly described previously (3,4,7,12) and have been characterized by IS6110 RFLP and spoligotyping (see Table S1 in the supplemental material).…”

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confidence: 99%

Russian “Successful” Clone B0/W148 of Mycobacterium tuberculosis Beijing Genotype: a Multiplex PCR Assay for Rapid Detection and Global Screening

et al. 2012

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dWe describe a multiplex PCR assay to detect the Mycobacterium tuberculosis Beijing genotype variant B0/W148, which is considered a "successful" clone of M. tuberculosis, widespread in Russia. Specificity and sensitivity of the assay were 100% based on the analysis of a collection of 516 M. tuberculosis isolates of different genotypes and origins. This assay may be used for accurate and simple detection and surveillance of this clinically and epidemiologically important variant of M. tuberculosis.

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“…Converging drug-resistant TB and HIV epidemics pose a serious problem and undermine the efforts of national TB control programs (5). There is growing evidence that drug resistance in these settings is strongly associated with the dominating Beijing family of strains (6,10,14,15,17,21,22).…”

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Detection of Resistance to Second-Line Antituberculosis Drugs by Use of the Genotype MTBDR sl Assay: a Multicenter Evaluation and Feasibility Study

Ignatyeva

Kontsevaya²,

Kovalyov³

et al. 2012

J Clin Microbiol

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eThe rate of multidrug-resistant (MDR) and extensively drug-resistant (XDR) tuberculosis (TB) has been steadily increasing in countries of the former USSR. The availability of rapid and reliable methods for the detection of drug resistance to second-line drugs is vital for adequate patient management. We evaluated the performance of the Genotype MTBDRsl assay compared to that of phenotypic drug susceptibility testing (Becton Dickinson Bactec MGIT 960 system) with a test panel of 200 Mycobacterium tuberculosis isolates at four sites in Eastern Europe. The interpretability of the Genotype MTBDRsl assay was over 95%. The sensitivity for the detection of resistance to fluoroquinolones, ethambutol, amikacin, and capreomycin varied between 77.3% and 92.3%; however, it was much lower for kanamycin (42.7%). The sensitivity for the detection of XDR TB was 22.6%. The test specificity was over 82% for all drugs. The assay presents a good screening tool for the rapid detection of resistance to individual second-line drugs and can be recommended for use in countries with a high burden of MDR/XDR TB. The sensitivity for the detection of kanamycin resistance needs improvement.

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Emerging multidrug resistant Mycobacterium tuberculosis strains of the Beijing genotype circulating in Russia express a pattern of biological properties associated with enhanced virulence

Cited by 69 publications

References 21 publications

Mycobacterium tuberculosis Strains of the Modern Sublineage of the Beijing Family Are More Likely To Display Increased Virulence than Strains of the Ancient Sublineage

Mycobacterium tuberculosis Strains of the Modern Sublineage of the Beijing Family Are More Likely To Display Increased Virulence than Strains of the Ancient Sublineage

Russian “Successful” Clone B0/W148 of Mycobacterium tuberculosis Beijing Genotype: a Multiplex PCR Assay for Rapid Detection and Global Screening

Detection of Resistance to Second-Line Antituberculosis Drugs by Use of the Genotype MTBDR sl Assay: a Multicenter Evaluation and Feasibility Study

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