Emerging of coagulase negative staphylococci as a cause of mastitis in dairy animals: An environmental hazard

El-Jakee, J.; Aref, Noha E.; Gomaa, Alaa; Elhariri, Mahmoud; Galal, Hussein M.; Omar, Sherif A.; Samir, Ahmed

doi:10.1016/j.ijvsm.2013.05.006

Cited by 61 publications

(56 citation statements)

References 21 publications

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“…However, CNS can cause persistent infections, which result in increased milk somatic cell count (SCC) and decreased milk quality [24]. The current results reported that CNS was the predominant isolate recovered from subclinical mastitis cows with percentage 65%, these result was in harmony with El-Jakee et al [25] who reported that CNS is generally high in subclinical mastitic samples, but low in samples from animals with clinical mastitis. In our study, S. chromogenes, S. epidermidis and S. haemolyticus were the most prevalent CNS with percentage 31%, 27% and 23% respectively.…”

Section: Discussionsupporting

confidence: 83%

Coagulase Gene Typing with Emphasis on Methicillin-Resistance Staphylococci: Emergence to Public Health

Hamza¹,

Dorgham²,

Arafa³

2015

AID

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Emerging antimicrobial resistance among CNS is a concern in veterinary and human medicine. Coagulase test is considered as the key test to differentiate staphylococci to two groups, coagulase positive staphylococci (CPS) and coagulase negative staphylococci (CNS). A total of 200 Staphylococci strains were isolated with percentage 66.7% (200/300) from quarter milk samples. The total of S. aureus strains are 70 with percentage 35% (70/200). Among 70 strains of S. aureus, 30 strains are coagulase positive S. aureus with percentage 43% (30/70) and coagulase negative S. aureus 57% (40/70). CNS other than S. aureus was detected with percentage 65% (130/200) from subclinical mastitic cows. We examine sixty isolates of staphylococci recovered from subclinical mastitis in dairy cattle which divided as ten isolates of coagulase positive S. aureus (CP S. aureus), ten isolates of coagulase negative S. aureus (CN S. aureus) and forty isolates of coagulase negative staphylococci (CNS) which identified using API- Staph Kits as S. chromogenes, S. simulans, S. haemolyticus, S. epidermidis and S. cohnii. The genotypic detection of coa gene and mecA gene was screened in CP S. aureus, CN S. aureus and CNS.

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Section: Discussionsupporting

confidence: 83%

Coagulase Gene Typing with Emphasis on Methicillin-Resistance Staphylococci: Emergence to Public Health

Hamza¹,

Dorgham²,

Arafa³

2015

AID

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“…In Egypt coagulase-negative Staphylococci were isolated from the examined subclinical mastitic sheep and goats with percentages of 50 and 55.6% respectively (El-Jakee et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Mastitis Pathogens and Their Resistance Against Antimicrobial Agents in Awassi Sheep in Al-Balqa Province of Jordan

Hawari

Obeidat

Awaisheh

et al. 2014

American Journal of Animal and Veterinary Sciences

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The primary objective of this study was to establish data on mastitis in Awassi Sheep in Al-Balqa Province of Jordan. Milk samples were collected from 260 lactating ewes that selected randomly from eight flocks. California Mastitis Test (CMT) gave result with 220 milk samples; 122 samples (55.5%) showed positive CMT. Infection with some bacterial species was associated with positive CMT. About 26% of the ewes revealed clinical signs of mastitis. The highest percentage of bacterial count, which range from 3×10 2 to <3.0 10 3 cfu mL −1 was founded in the milk samples. The most predominant bacteria isolated were Staphylococcus aureus, Streptococcus agalactiae, Streptococcus spp., Escherichia coli, Corynebacterium spp. and Coagulase negative Staphylococci. Sensitivity tests were applied to different isolated strains., Gentamycim, Ampicillin and Tetracycline were the most effective antimicrobial agents against the bacterial isolates.

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“…The importance of CoNS has become more noticeable in the dairy herds that use an effective mastitis control programs as they cause a significant reduction in milk quality and also an udder tissue injury (El‐Jakee et al ). Herein, CoNS was isolated with a lower prevalence rate (24%) than that reported in Iraq (39.3%) (Al‐Zainy and Al‐Jeburii ) and Mexico (42%) (León‐Galván et al ).…”

Section: Discussionmentioning

confidence: 99%

Propidium monoazide conventional PCR and DNA sequencing: detection of negative culture bacterial pathogens causing subclinical mastitis

Farhan¹,

El-Hamid

Hassan

2020

J Appl Microbiol

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Aims This study was conducted to early detect the negative culture bacterial pathogens causing subclinical mastitis for the fast diagnosis of the disease and the reduction of some milk‐transmitted pathogenic bacteria to human consumers. Methods and Results A total of 171 positive California mastitis test (CMT) milk samples collected from asymptomatic dairy cows in Sharkia Governorate, Egypt were examined by conventional bacteriological methods. The obtained results revealed that Streptococcus species (77·2%), followed by Staphylococcus species (48·6%) and Escherichia coli (25·7%) were the most predominant bacterial pathogens isolated from positive culture milk samples, whereas Enterobacter and Pseudomonas species were the lowest ones (1·2%, for each). Herein, 13 (7.6%) negative culture milk samples were subjected to propidium monoazide (PMA) conventional PCR assay, followed by DNA sequencing of purified PCR amplicons. Sequence analysis identified seven different types of negative culture bacterial pathogens comprising as following; 4 Enterococcus hirae, 2 Bacillus cereus, 2 Staphylococcus aureus, 1 Bacillus mycoides, 1 Bacillus subtilis, 1 Enterococcus faecium and 1 Escherichia coli. Conclusions All the detected negative culture bacterial pathogens by PMA‐PCR assay, followed by DNA sequencing were incriminated in causing subclinical mastitis disease and had serious implications on human public health through consumption of milk contaminated with those recovered bacterial pathogens. Significance and Impact of the Study The used methods could be useful in the routine detection of negative culture bacterial pathogens present in milk and consequently, it will help in the rapid diagnosis of subclinical mastitis disease and the reduction of many milk‐transmitted diseases to human.

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Emerging of coagulase negative staphylococci as a cause of mastitis in dairy animals: An environmental hazard

Cited by 61 publications

References 21 publications

Coagulase Gene Typing with Emphasis on Methicillin-Resistance Staphylococci: Emergence to Public Health

Coagulase Gene Typing with Emphasis on Methicillin-Resistance Staphylococci: Emergence to Public Health

Prevalence of Mastitis Pathogens and Their Resistance Against Antimicrobial Agents in Awassi Sheep in Al-Balqa Province of Jordan

Propidium monoazide conventional PCR and DNA sequencing: detection of negative culture bacterial pathogens causing subclinical mastitis

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