Emerging Roles of Urine-Derived Components for the Management of Bladder Cancer: One Man’s Trash is Another Man’s Treasure

Minkler, Sarah; Lucien, Fabrice; Kimber, Michael J.; Bourgois-Mochel, Agnes; Musser, Margaret; Johannes, Chad M.; Frank, Igor; Cheville, John C.; Allenspach, Karin; Mochel, Jonathan P.

doi:10.20944/preprints202011.0364.v1

Cited by 3 publications

(3 citation statements)

References 85 publications

(112 reference statements)

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“…Kang et al, 2020 also illustrate the taxonomic classifications, with suggested treatments for different MIBC subtypes based on molecular biomarkers [ 109 ]. The combination of neoadjuvant therapy with immune checkpoint inhibitors (designed for suitable biomarker expression) is currently being tested and is expected to increase efficacy in treatments [ 110 ].…”

Section: Bladder Cancer Therapiesmentioning

confidence: 99%

The Role of Androgens and Androgen Receptor in Human Bladder Cancer

et al. 2021

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Bladder cancer (urothelial carcinoma) is one of the most frequently diagnosed neoplasms, with an estimated half a million new cases and 200,000 deaths per year worldwide. This pathology mainly affects men. Men have a higher risk (4:1) of developing bladder cancer than women. Cigarette smoking and exposure to chemicals such as aromatic amines, and aniline dyes have been established as risk factors for bladder cancer and may contribute to the sex disparity. Male internal genitalia, including the urothelium and prostate, are derived from urothelial sinus endoderm; both tissues express the androgen receptor (AR). Several investigations have shown evidence that the AR plays an important role in the initiation and development of different types of cancer including bladder cancer. In this article, we summarize the available data that help to explain the role of the AR in the development and progression of bladder cancer, as well as the therapies used for its treatment.

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Section: Bladder Cancer Therapiesmentioning

confidence: 99%

The Role of Androgens and Androgen Receptor in Human Bladder Cancer

et al. 2021

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“…Canine bladder cancer organoids were previously described and exposed to anticancer drugs to describe their potential role in research and precision medicine (Elbadawy et al, 2019). Since canine bladder cancer is a well-established model for human muscle-invasive bladder cancer (Knapp et al, 2020), canine bladder cancer organoids represent a valuable model for translational preclinical research (Minkler et al, 2021). In addition, Elbadawy et al has recently described healthy canine bladder organoids (Elbadawy et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

A Multi-Tissue Comparison and Molecular Characterization of Canine Organoids

Zdyrski

Gabriel

Ospina

et al. 2022

Preprint

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Organoids are 3-dimensional (3D) stem cell-derived cell culture lines that offer a variety of technical advantages compared to traditional 2-dimensional (2D) cell cultures. Although murine models have proved useful in biomedical research, rodent models often fail to adequately mimic human physiology and disease progression, resulting in poor preclinical prediction of therapeutic drug efficacy and toxicity. With the advent of organoid technology, many of these challenges can be overcome. Previously, the use of canine organoids in drug testing and disease modeling was limited to organoids originating from the intestine, liver, kidney, and urinary bladder. Here, we report the cultivation, maintenance, and molecular characterization of three novel adult-stem cell-derived canine organoid cell lines, including the endometrium, lung, and pancreas, in addition to previously reported kidney, bladder, and liver organoids from two genetically related canines. Six tissues and organoid lines from each donor were characterized using bulk RNASeq, allowing for a unique, multi-organ comparison between these two individuals and identification of specific cell types such as glandular epithelial cells in endometrial organoids.

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“…Organoid technology has expanded recently from commonly used mice and human models to canines (Ambrosini et al, 2020;Chandra et al, 2019;Gabriel et al, 2022;Minkler et al, 2021;Mochel et al, 2018;Nantasanti et al, 2015) and few other vertebrates. However, the only reptilian (sensu lato) organoids (i.e., excluding those derived from chicken intestines (Zhao et al, 2022)), were generated from snake venom glands (Post et al, 2020), representing only a fraction of the vast Tree of Life.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the First Turtle Organoids: A Model for Investigating Unique Adaptations with Biomedical Potential

Zdyrski

Gabriel

Gessler

et al. 2023

Preprint

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Painted turtles are remarkable for their well-developed freeze tolerance and associated resilience to hypoxia/anoxia, oxidative stress, and ability to supercool. They are, therefore, are an ideal model for biomedical research on hypoxia-induced injuries (such as strokes), tissue cooling during extensive surgeries, and organ cryopreservation. Yet, the seasonal reproduction and slow maturation of turtles hinder basic and applied biomedical research. To overcome these limitations, we have developed for the first time, adult stem cell-derived turtle hepatic organoids, which provide 3D self-assembled structures that closely mimic their original tissue and allow for in vitro testing and experimentation without constantly harvesting donor tissue and screening offspring. Our pioneering work with turtles represents the first for this vertebrate Order and complements the only other organoid lines from non-avian reptiles, which were produced from snake venom glands. Here we report the isolation and characterization of hepatic organoids derived from painted, snapping, and spiny softshell turtles spanning ~175 million years of evolution, with a subset being preserved in a biobank. Morphological and transcriptomics revealed organoid cells resembling cholangiocytes, which was then compared to the tissue of origin. Deriving organoids from multiple turtle species and life stages demonstrates that our organoid culturing techniques are broadly applicable to chelonians, permitting the development of functional genomic tools currently missing in most herpetological research. When combined with genetic editing, this platform will further support studies of genome-to-phenome mapping, gene function, genome architecture, and adaptive responses to climate change, among others. We discuss the unique abilities of painted turtles, including their overwintering potential, an adaptation with important implications for ecological, evolutionary, and biomedical research.

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Emerging Roles of Urine-Derived Components for the Management of Bladder Cancer: One Man’s Trash is Another Man’s Treasure

Cited by 3 publications

References 85 publications

The Role of Androgens and Androgen Receptor in Human Bladder Cancer

The Role of Androgens and Androgen Receptor in Human Bladder Cancer

A Multi-Tissue Comparison and Molecular Characterization of Canine Organoids

Characterization of the First Turtle Organoids: A Model for Investigating Unique Adaptations with Biomedical Potential

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