The objective of this study is to find the effect on DI-CI naturally aspirated engine influenced by Manilkara Zapota biodiesel blends doped with green synthesized silver nanoparticles. The Manilkara Zapota biodiesel is used as base fuel and green synthesized silver nanoparticle as nanoadditive. The nanoparticle is prepared with the leaf extracts of Manilkara Zapota. The different test fuel blends are prepared with different concentrations of 40, 80, and 120 ppm of silver nanoparticles such as B100, B20, B20AgNP40ppm, B20AgNP80ppm, B20AgNP120ppm and diesel. The fuel properties are measured as per ASTM standard and compared the results with and without nanoparticles are presented in this paper. To validate the test fuel by conduct the experimental investigation on a single cylinder DI-CI naturally aspirated engine loaded with eddy current dynamometer attached with combustion pressure transducer. The experimental results proved test fuel B20AgNP80ppm exhibits better results compared to other test fuels. The brake thermal efficiency of 33.31% was obtained for B20AgNP80ppm fuel which is 2.83% and 1.02% higher than B20 and diesel. The maximum reduction of CO obtained for the fuel blend B20 with addition of 80 ppm of AgNP were 33.33% and 37.5% compared to B20 and diesel. The UHC emission reduced by 32.6% and 38% for B20AgNP80ppm compared to B20 and diesel. It is also found that lower NOx and smoke for B20AgNP80ppm blend compared to B20 and diesel. The maximum cylinder pressure and heat release rate were achieved for B20AgNP80ppm compared to other blends.