Emission spectra from copper proteins containing type-3 centres

Bacci, Mauro; Baldecchi, Maria Grazia; Fabeni, P.; Linari, R.; Pazzi, G.P.

doi:10.1016/0301-4622(83)80006-4

Cited by 8 publications

(2 citation statements)

References 18 publications

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“…After this work had been completed and this manuscript was in preparation, a manuscript appeared [17] reporting a similar fluorescence emission (emission maxima: 415-445 mn; excitation maxima: 325-345 nm, lifetime c 10 ns) from 3 proteins that contain binuclear Cu centers (tyrosinase, ceruloplasmin and hemocyanin). These observations strengthen our hypothesis that the emission is associated with type 3 Cu centers.…”

Section: Methodsmentioning

confidence: 99%

Fluorescence associated with the type 3 copper center of laccase

et al. 1983

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Laccases isolated from the lacquer tree Rhus vernicifera and the fungus Polyporus versicolor show fluorescence emission near 420 run and phosphorescence emission in the 440-465 mn region. The fluorescence and phosphorescence excitation spectra for both laccases show maxima in the 315-330 mn range, a spectral region corresponding to the absorbance maxima for the type 3 binuclear Cu centers of the two enzymes. Additional evidence is presented for the association of the newly discovered emissions with the type 3 Cu centers of the two laccases. LactaseType 3 Cu Fluorescence Phosphorescence

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Section: Methodsmentioning

confidence: 99%

Fluorescence associated with the type 3 copper center of laccase

et al. 1983

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“…Some authors attributed the emission at 415 nm, which was visible in all silk solutions as a result of exciting at 325 nm, to copper proteins containing type-3 centres. 16 In this work, we have not investigated in detail the presence or absence of copper ions in SF samples, but different researchers found that copper and other inorganic ions (Fe, Na, Ca, K, Zn, Mn) are present in the glands of the silkworm and play an important role in the folding process of broin during the natural spinning process. 17 Therefore, in our case, we could hypothesize that the presence of a low concentration of copper(II) ions complexed to specic peptide sequences in broin, i.e.…”

Section: Colorimetric Analysis Of Rhb-modied Cocoons and Regenerated...mentioning

confidence: 99%

Bio-doping of regenerated silk fibroin solution and films: a green route for biomanufacturing

Sagnella¹,

Chieco

Virgilio

et al. 2014

RSC Adv.

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Silk fibroin (SF) is a natural biocompatible material that can be integrated in a variety of photonic systems and optoelectronics: i.e. organic lasing from dye-doped nano-structured silk film. In this context, biological incorporation of doping molecules into SF by means of feeding silk worms with dyes to their diet could be an innovative and eco-sustainable approach to obtain doped SF substrates, thus avoiding additional chemical processes and post-treatments of the protein solution. In the present work, we demonstrated that SF regenerated solutions and films containing rhodamine B (RhB) could be successfully obtained from the cocoons of Bombyx mori fed with a RhB-added diet (RhB-md-SF). Comparative analyses of optical and vibration characteristics of the RhB-md-SF solution and films with those of white SF blended with RhB (RhB-d-SF) revealed significant differences, suggesting that the silkworm's metabolism could be involved in the binding mechanism of SF with RhB. In conclusion, we observed that the doping diet is a promising method for the green fabrication of SF-based optically active materials, and it opens novel routes for silk-based biophotonics.

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