“…(Zimmerman, 1993); the use of the method to quantify the methane emission has been debated in some studies of Pinares-Patiño et al (2008) and Pinares-Patiño et al (2011); In Vitro Gas Production Technique for Methane Measurements (requires access to fresh rumen fluid, which is typically obtained from fistulated cows or other ruminants); The CO 2 Technique (estimates methane emissions from livestock based on the use of CO 2 as a tracer gas (Madsen et al, 2010)); Methods Based on Whole Buildings or Areas (can be divided into non-micrometeorological techniques and micrometeorological techniques defined as measuring fluxes of gas in the free atmosphere and relating these fluxes to animal emissions (Harper et al, 2011); Combined Feeder and CH 4 Analyzer (is a patented system which combines an automatic feeding system with measurements of CH 4 and CO 2 ), proxy-methods (are correlating the methane emissions with parameters that can be measured in easily obtainable biological samples like milk or feces). Other methods for estimating the methane emissions are: the large dynamic chamber (Enishi and Osada, 2012), chambers made of aluminum which measured 10 cm tall used for flux measurement from swine slurry (Lovanh et al, 2010), closed chamber (test containers with flexible roof ) methodology at a pilot-scale for measurements of gas emissions from cattle stored slurry (Rodhe et al, 2009). When the measurements are not possible, the total emissions have to be assessed by IPCC method or methane models (Storm et al, 2012).…”