Empfindlichkeit einiger Farbstoffe zum Nachweis von β-Granula in den Inselzellen des Pankreas während der Ontogenese

Denffer, Hans v.; Mertz, M.

doi:10.1007/bf00305701

Cited by 11 publications

(1 citation statement)

References 37 publications

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“…Von Denffer & Mertz (1972) observed that B-cell granules of mouse pancreas were not stained by Alcian Blue and attributed this to the absence of sulphur acids associated with granules. Von Denffer (1973) investigated the"effects of pretreating pancreatic B-cell granules with histochemical blocking agents prior to staining with Aldehyde Fuchsin and concluded that the following moieties were not responsible for binding Aldehyde Fuchsin: thiols, disulphides, tyrosyl-hydroxyls, phospholipids and amino groups.…”

Section: Introductionmentioning

confidence: 95%

A histochemical investigation of the mechanism of Aldehyde Fuchsin staining of pancreatic B-cell granules

Cole

Nettleton

1988

Histochem J

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The reaction mechanism by which Aldehyde Fuchsin selectively stains pancreatic B-cell granules is unknown. The participation of either insulin or proinsulin in the reaction is debatable; the stain may be bound by other components of the B-cell granule or its membrane. Sections of pancreas were stained with a variety of basic stains and specific histochemical reagents with and without appropriate blocking agents. No evidence for strong tissue anions associated with the B-cell granule could be found. Aldehyde Fuchsin staining was not abolished by lowering the pH below the point at which all known tissue anions should be protonated. There was no evidence that the Aldehyde Fuchsin staining solution itself generates reactive groups in the tissue. The results of this investigation support a non-ionic, possibly covalent mechanism for Aldehyde Fuchsin staining of pancreatic B-cell granules.

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Section: Introductionmentioning

confidence: 95%