Employing AgNPs doped amidoxime-modified polyacrylonitrile (PAN-oxime) nanofibers for target induced strand displacement-based electrochemical aptasensing of CA125 in ovarian cancer patients

Farzin, Leila; Sadjadi, Sodeh; Shamsipur, Mojtaba; Sheibani, Shahab; Mousazadeh, Mohammad H.

doi:10.1016/j.msec.2018.12.108

Cited by 56 publications

(25 citation statements)

References 57 publications

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“…Additionally, aptamers are more stable than antigens, and can easily recover their active spatial configuration after usage thereby allowing for the reuse of the same electrode multiple times [ 85 ]. Based on how the immobilized aptamers interact with the target analyte, Han and coworkers proposed the following categories: (a) spatial configuration rearrangement of aptamers based on target interaction [ 86 , 87 , 88 ]; (b) sandwich type interactions [ 89 , 90 , 91 ]; (c) dissociation or displacement of aptamers through target interaction [ 92 , 93 ]; and (d) competitive replacement of aptamers [ 14 , 94 , 95 , 96 ].…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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With the rise in public health awareness, research on point-of-care testing (POCT) has significantly advanced. Electrochemical biosensors (ECBs) are one of the most promising candidates for the future of POCT due to their quick and accurate response, ease of operation, and cost effectiveness. This review focuses on the use of metal nanoparticles (MNPs) for fabricating ECBs that has a potential to be used for POCT. The field has expanded remarkably from its initial enzymatic and immunosensor-based setups. This review provides a concise categorization of the ECBs to allow for a better understanding of the development process. The influence of structural aspects of MNPs in biocompatibility and effective sensor design has been explored. The advances in MNP-based ECBs for the detection of some of the most prominent cancer biomarkers (carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), Herceptin-2 (HER2), etc.) and small biomolecules (glucose, dopamine, hydrogen peroxide, etc.) have been discussed in detail. Additionally, the novel coronavirus (2019-nCoV) ECBs have been briefly discussed. Beyond that, the limitations and challenges that ECBs face in clinical applications are examined and possible pathways for overcoming these limitations are discussed.

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Section: Electrochemical Biosensorsmentioning

confidence: 99%

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

et al. 2020

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“…In addition, such a design (electrochemical signal-off strategy) has a main limitation of negative signals. To overcome the aforementioned disadvantage, signal-on electrochemical biosensors were duch improved signaling; as the background current observed in the absence of a target is reduced, the gain of such a sensor, at least in theory, increases without limit [ 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

A nanoscale genosensor for early detection of COVID-19 by voltammetric determination of RNA-dependent RNA polymerase (RdRP) sequence of SARS-CoV-2 virus

Farzin¹,

Sadjadi²,

Sheini³

et al. 2021

Microchim Acta

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Graphical abstract A voltammetric genosensor has been developed for the early diagnosis of COVID-19 by determination of RNA-dependent RNA polymerase (RdRP) sequence as a specific target of novel coronavirus. The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) uses an RdRP for the replication of its genome and the transcription of its genes. Here, the silver ions (Ag + ) in the hexathia-18-crown-6 (HT18C6) were used for the first time as a redox probe. Then, the HT18C6(Ag) incorporated carbon paste electrode (CPE) was further modified with chitosan and PAMAM dendrimer-coated silicon quantum dots (SiQDs@PAMAM) for immobilization of probe sequences (aminated oligonucleotides). The current intensity of differential pulse voltammetry using the redox probe was found to decrease with increasing the concentration of target sequence. Based on such signal-off trend, the proposed genosensor exhibited a good linear response to SARS-CoV-2 RdRP in the concentration range 1.0 pM–8.0 nM with a regression equation I (μA) = − 6.555 log [RdRP sequence] (pM) + 32.676 ( R 2 = 0.995) and a limit of detection (LOD) of 0.3 pM. The standard addition method with different spike concentrations of RdRP sequence in human sputum samples showed a good recovery for real sample analysis (> 95%). Therefore, the developed voltammetric genosensor can be used to determine SARS-CoV-2 RdRP sequence in sputum samples. PAMAM-functionalized SiQDs were used as a versatile electrochemical platform for the SARS-CoV-2 RdRP detection based on a signal off sensing strategy. In this study, for the first time, the silver ions (Ag + ) in the hexathia-18-crown-6 carrier were applied as an electrochemical probe. Supplementary Information The online version contains supplementary material available at 10.1007/s00604-021-04773-6.

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“…The sDNA displacement resulted in the diminishing MB peak currents detected by DPV within the 0.01 to 350 U mL −1 CA125 dynamic linear range. Lysozyme, IgG, IgA, BSA and serum haemoglobin (Hb) did not significantly influence the CA125 analysis [161].…”

Section: Carcinoma Antigen 125mentioning

confidence: 97%

“…The sensor was exposed to CA125, and its recognition by the aptamer released the MB-labelled sDNA into solution. The sDNA displacement resulted in the diminishing MB peak currents detected by DPV A nanocomposite electrode modification exploiting amidoxime-modified polyacrylonitrile electrospinned nanofibers (NFs) decorated with Ag nanoparticles (AgNPs-PAN-oxime NFs) allowed the 0.0042 U mL −1 DPV detection of CA125 and its clinical applications, such as analysis of CA125 positive and negative human serum samples comparable to ELISA [161]. In sensor construction, the indium tin oxide (ITO) electrode was covered by AgNPs-PAN NFs, chemically modified to bear amidoxime groups, to which the aminated aptamer was coupled via glutaraldehyde (GA) cross-linking.…”

Section: Carcinoma Antigen 125mentioning

confidence: 99%

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

Malecka

Mikuła

Ferapontova

2021

Sensors

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Improved outcomes for many types of cancer achieved during recent years is due, among other factors, to the earlier detection of tumours and the greater availability of screening tests. With this, non-invasive, fast and accurate diagnostic devices for cancer diagnosis strongly improve the quality of healthcare by delivering screening results in the most cost-effective and safe way. Biosensors for cancer diagnostics exploiting aptamers offer several important advantages over traditional antibodies-based assays, such as the in-vitro aptamer production, their inexpensive and easy chemical synthesis and modification, and excellent thermal stability. On the other hand, electrochemical biosensing approaches allow sensitive, accurate and inexpensive way of sensing, due to the rapid detection with lower costs, smaller equipment size and lower power requirements. This review presents an up-to-date assessment of the recent design strategies and analytical performance of the electrochemical aptamer-based biosensors for cancer diagnosis and their future perspectives in cancer diagnostics.

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Employing AgNPs doped amidoxime-modified polyacrylonitrile (PAN-oxime) nanofibers for target induced strand displacement-based electrochemical aptasensing of CA125 in ovarian cancer patients

Cited by 56 publications

References 57 publications

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

Metal Nanoparticles for Electrochemical Sensing: Progress and Challenges in the Clinical Transition of Point-of-Care Testing

A nanoscale genosensor for early detection of COVID-19 by voltammetric determination of RNA-dependent RNA polymerase (RdRP) sequence of SARS-CoV-2 virus

Design Strategies for Electrochemical Aptasensors for Cancer Diagnostic Devices

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