Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana

Nikolić, Jelena S.; Nešić, Andrijana; Kull, Skadi; Schocker, Frauke; Jappe, Uta; Gavrović-Jankulović, Marija

doi:10.1016/j.jprot.2018.01.007

Cited by 18 publications

(7 citation statements)

References 45 publications

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“…Another situation where proteins need to be maintained in their native form is the identification of novel allergens. In this field, numerous publications described protocols for the discovery of low-abundance allergens [70,71]. The use of entire, nondenatured proteins is also mandatory to evidence certain cellular mechanisms after enrichment with CPLL technology [72].…”

Section: Combination With Analytical Methodsmentioning

confidence: 99%

Combinatorial peptides: A library that continuously probes low‐abundance proteins

Boschetti¹,

Zilberstein

Righetti

2021

Electrophoresis

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After a decade of experimental applications, it is the objective of this review to make a point on combinatorial peptide ligand libraries dedicated to low‐abundance proteins from animals to plants and to microorganism proteomics. It is, thus, at the light of the recent technical developments and applications that we will examine the state of the art, its usage within the scientific community, and its openness to unexplored fields. The improvements of the methodology and its implementation in connection with analytical determinations of combinatorial peptide ligand library (CPLL)‐treated samples are extensively reviewed and commented upon. Relevant examples covering few critical aspects describe the performance of the technology. Finally, a reflection on the technological future is attempted in particular by involving new concepts adapted to the limited availability of certain biological samples.

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Section: Combination With Analytical Methodsmentioning

confidence: 99%

Combinatorial peptides: A library that continuously probes low‐abundance proteins

Boschetti¹,

Zilberstein

Righetti

2021

Electrophoresis

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“…2-D immunoblot analysis was rarely applied for detection of allergens e.g., for determination of the catalase allergen from banana ( 30 ). For allergogram analysis banana protein extract was mixed with buffer containing Tris-HCl, pH 6.8, glycerol and bromphenolblue and incubated for 5 min at 95°C.…”

Section: Methods For the Detection And Analysis Of Allergensmentioning

confidence: 99%

“…2-DE was applied for separation of proteins from banana fruit extract ( 30 ). In the procedure, banana fruit proteins were resolved by 1-D SDS-PAGE (4% stacking and 12% resolving gel) and then electrotransferred onto a nitrocellulose membrane.…”

Section: Methods For the Detection And Analysis Of Allergensmentioning

confidence: 99%

Review of New Trends in the Analysis of Allergenic Residues in Foods and Cosmetic Products

Tuzimski

Petruczynik

2020

Journal of AOAC INTERNATIONAL

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Background Allergies represent an important health problem in industrialized countries. Allergen sensitization is an important risk factor for the development of allergic diseases; thus, the identification of an individual’s allergen sensitization is essential for the diagnosis and treatment of diseases. Objective This review compares different modern methods applied for the analysis of allergens in various matrices (from 2015 to the end of September 2019). Conclusions Immunological methods are still most frequently used for detection of allergens. These methods are sensitive, but the lack of specificity and cross-reaction of some antibodies can still be a relevant source of errors. DNA-based methods are fast and reliable for determination of protein allergens, but the epitopes of protein allergens with posttranslational modifications and their changes, originated during various processing, cannot be identified through the use of this method. Methods based on application of biosensors are very rapid and easy to use, and can be readily implemented as screening methods to monitor allergens. Recent developments of new high-resolution MS instruments are encouraging and enable development in the analysis of allergens. Fast, very sensitive, reliable, and accurate detection and quantification of allergens in complex samples can be used in the near future. Mass spectrometry coupled with LC, GC, or electrophoretic methods bring additional advances in allergen analysis. The use of LC-MS or LC-MS/MS for the quantitative detection of allergens in various matrices is at present gaining acceptance as a protein-based confirmatory technique over the routinely performed enzyme-linked immunosorbent assays.

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“…In addition to transcriptomics, several studies have been conducted to study the proteome of banana plants for the characterization and analysis of genetic variations observed in various banana varieties. Proteome analysis provided insights into the molecular dynamics of key proteins and their modulations at the post-transcriptional and post-translational levels during the vital processes of Foc resistance, drought tolerance, cold stress tolerance, fruit ripening, identification of banana allergens, etc ( Vanhove et al., 2012 ; Li et al., 2015 ; Nikolic et al., 2018 ; Dong et al., 2019 ; Bhuyian et al., 2020 ). Thus, understanding banana genetics through an integrative approach that involves comparative genomics in conjunction with transcriptomics and proteomics may provide better access to the genes and their cis-regulatory elements for the domestication of banana ‘new generations’ through breeding and for the development of genetically modified banana with elite traits.…”

Section: Integration Of Omics Studies For Banana Improvementmentioning

confidence: 99%

Biotechnological interventions in banana: current knowledge and future prospects

Justine

Kaur

Savita

et al. 2022

Heliyon

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Employment of proteomic and immunological based methods for the identification of catalase as novel allergen from banana

Cited by 18 publications

References 45 publications

Combinatorial peptides: A library that continuously probes low‐abundance proteins

Combinatorial peptides: A library that continuously probes low‐abundance proteins

Review of New Trends in the Analysis of Allergenic Residues in Foods and Cosmetic Products

Biotechnological interventions in banana: current knowledge and future prospects

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