EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin

Shi, Shengjun; Li, Qiao; Cao, Qiuying; Diao, Yong; Zhang, Yao; Yue, Lei; Wei, Liang

doi:10.3390/ijms21041349

Cited by 22 publications

(16 citation statements)

References 35 publications

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“…The downregulation of β 1 -integrin may inhibit cell adhesion to the ECM. In addition, MCF-7 breast cancer cells exposed to the rotating wall vessel (RWV) showed a decrease in β 1 -integrin protein [34], supporting the recent finding that focal adhesion is reduced in space and under s-µg conditions [35].…”

Section: Extracellular Matrix Proteins Altered By R-µg Exposuresupporting

confidence: 68%

The CellBox-2 Mission to the International Space Station: Thyroid Cancer Cells in Space

Melnik

Krüger

Schulz

et al. 2021

IJMS

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A spaceflight to the International Space Station (ISS) is a dream of many researchers. We had the chance to investigate the effect of real microgravity (CellBox-2 Space mission) on the transcriptome and proteome of FTC-133 human follicular thyroid cancer cells (TCC). The cells had been sent to the ISS by a Falcon 9 rocket of SpaceX CRS-13 from Cape Canaveral (United States) and cultured in six automated hardware units on the ISS before they were fixed and returned to Earth. Multicellular spheroids (MCS) were detectable in all spaceflight hardware units. The VCL, PXN, ITGB1, RELA, ERK1 and ERK2 mRNA levels were significantly downregulated after 5 days in space in adherently growing cells (AD) and MCS compared with ground controls (1g), whereas the MIK67 and SRC mRNA levels were both suppressed in MCS. By contrast, the ICAM1, COL1A1 and IL6 mRNA levels were significantly upregulated in AD cells compared with 1g and MCS. The protein secretion measured by multianalyte profiling technology and enzyme-linked immunosorbent assay (AngiogenesisMAP®, extracellular matrix proteins) was not significantly altered, with the exception of elevated angiopoietin 2. TCC in space formed MCS, and the response to microgravity was mainly anti-proliferative. We identified ERK/RELA as a major microgravity regulatory pathway.

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Section: Extracellular Matrix Proteins Altered By R-µg Exposuresupporting

confidence: 68%

The CellBox-2 Mission to the International Space Station: Thyroid Cancer Cells in Space

Melnik

Krüger

Schulz

et al. 2021

IJMS

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“…In breast cancer, Wnt3 overexpression can promote EMT-like phenotype through activating the Wnt/β-catenin signaling pathway [ 47 ]. EMT involves the down-regulation of E-cadherin and plays a vital role during the early stage of cancer metastasis [ 32 , 34 , 48 ]. EMT may result in the loss of intercellular connections of cancer cells, upregulation of contractile cytoskeleton components and remove the benign primary on account of epithelium; these changes make EMT a key factor that should not be neglected when studying the development of cancer [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Tumor Inhibitory Effect of Long Non-coding RNA LOC100505817 on Gastric Cancer

Zheng

Kang

Cheng

et al. 2021

Pathol. Oncol. Res.

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Gastric cancer (GC) is one of the major malignancies worldwide. Emerging evidence has revealed the potential involvement of long noncoding RNA (lncRNA) in human genetic disorders and cancer, but the role of LOC100505817 remains unknown. Thus, in this study, we isolated tissues from GC patients to characterize the functional importance of LOC100505817 in GC tumorigenesis. We also proposed a hypothesis that the regulation of Wnt/β-catenin pathway by LOC100505817 was regulated by miR-20a-mediated WT1. After the collection of cancer tissues and adjacent tissues were obtained from GC patients, expression of LOC100505817, Wnt/β-catenin pathway- and EMT-related genes was quantified. Ectopic expression and knockdown experiments were applied in order to investigate the protective role of LOC100505817 in the progression of GC. Subsequently, cell viability, flow cytometry for apoptosis and cell cycle were detected via CCK-8, while migration and invasion were determined using scratch test and Transwell assay respectively. Then interactions among LOC100505817, miR-20a and WT1 were explored by dual luciferase reporter gene assay, RNA pull down assay and RNA binding protein immunoprecipitation (RIP) assay. The results found poor expression LOC100505817 was poorly expressed in GC cells and tissues. Overexpressed LOC100505817 resulted in the significant reduction of cell proliferation, migration and invasion as well as the expression of Wnt2b, β-catenin, CyclinD1, N-cadherin, Vimentin and snail, while increased cell apoptosis along with the expression of E-cadherin. Wnt/β-catenin pathway and EMT in GC cells were suppressed by LOC100505817 through miR-20a-inhibted WT1. In summary, our results provided evidence suggesting that LOC100505817 inhibits GC through LOC100505817-mediated inhibition of Wnt/β-catenin pathway, that leads to the overall restraining of GC cell proliferation, migration and invasion through miR-20a-reduced WT1.

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“…For example, in tumor cells, the expression of ICAM1 protein and the performance of focal adhesion such as vinculin and cofilin are different between real and ground-based simulated microgravity [82]. The expression of E-cadherin and cell adhesion state in HUVEC and MCF-7 cells showed different under simulated microgravity and overloading condition [110]. In addition, bauer et al used mass spectrometry and mRNA microarray to compare the different effect of microgravity on sialylation of adhesion proteins in FTC-133 thyroid cancer cells, MCF7 cells, and EA-hy926 cells [111].…”

Section: Discussionmentioning

confidence: 99%

The Impact of Spaceflight and Simulated Microgravity on Cell Adhesion

Lin

Zhang

Wei

et al. 2020

IJMS

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Microgravity induces a number of significant physiological changes in the cardiovascular, nervous, immune systems, as well as the bone tissue of astronauts. Changes in cell adhesion properties are one aspect affected during long-term spaceflights in mammalian cells. Cellular adhesion behaviors can be divided into cell–cell and cell–matrix adhesion. These behaviors trigger cell–cell recognition, conjugation, migration, cytoskeletal rearrangement, and signal transduction. Cellular adhesion molecule (CAM) is a general term for macromolecules that mediate the contact and binding between cells or between cells and the extracellular matrix (ECM). In this review, we summarize the four major classes of adhesion molecules that regulate cell adhesion, including integrins, immunoglobulin superfamily (Ig-SF), cadherins, and selectin. Moreover, we discuss the effects of spaceflight and simulated microgravity on the adhesion of endothelial cells, immune cells, tumor cells, stem cells, osteoblasts, muscle cells, and other types of cells. Further studies on the effects of microgravity on cell adhesion and the corresponding physiological behaviors may help increase the safety and improve the health of astronauts in space.

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EMT Transcription Factors Are Involved in the Altered Cell Adhesion under Simulated Microgravity Effect or Overloading by Regulation of E-cadherin

Cited by 22 publications

References 35 publications

The CellBox-2 Mission to the International Space Station: Thyroid Cancer Cells in Space

The CellBox-2 Mission to the International Space Station: Thyroid Cancer Cells in Space

Tumor Inhibitory Effect of Long Non-coding RNA LOC100505817 on Gastric Cancer

The Impact of Spaceflight and Simulated Microgravity on Cell Adhesion

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