Emu oil-based electrospun nanofibrous scaffolds for wound skin tissue engineering

Unnithan, Afeesh Rajan; Pichiah, P.B. Tirupathi; Gnanasekaran, Gopalsamy; Kalaiselvi, Seenivasan; Barakat, Nasser A.M.; Cha, Youn‐Soo; Jung, Che-Hun; Achiraman, Shanmugam; Kim, Hee Young

doi:10.1016/j.colsurfa.2012.09.029

Cited by 99 publications

(69 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, a twin peak observed at 1,730 and 1,703 cm −1 indicates the C=O stretching and the sharp peaks seen at 1,221 and 1,104 cm −1 represents the C‐O stretching alcohol groups . In both PU/olive oil and PU/olive oil/propolis/honey scaffold spectra, it was observed no addition bands formed but the intensity of peak was decreased in PU/olive oil and PU/olive oil/propolis/honey scaffold due to the formation of hydrogen bond . Further, the peak shift was observed in both scaffolds which indicate the strong interaction between PU, olive oil, honey, and propolis.…”

Section: Resultsmentioning

confidence: 91%

Tailor‐made multicomponent electrospun polyurethane nanofibrous composite scaffold comprising olive oil, honey, and propolis for bone tissue engineering

et al. 2018

View full text Add to dashboard Cite

The treatment for the bone diseases or defects such as tumor ablation, bone cysts and osteolysis were still challenging in clinical applications. Recently, the bone tissue engineering has emerged as a potential option for the treatment of bone defects. In this study, the nanofibrous composite scaffold consisting of polyurethane, olive oil, honey and propolis were fabricated through electrospinning method. The morphology of the nanofibrous scaffold indicated that nanofibers diameters were reduced with the addition of olive oil, honey and propolis into the Polyurethane (PU). The contact angle measurements showed that the behavior of PU/olive oil was found to hydrophobic (114° ± 1.528) and the PU/olive oil/honey/propolis scaffold rendered hydrophilic behavior (60° ± 1.528). FTIR and TG analysis revealed the interactions of PU with olive oil, honey/propolis and increased thermal stability of the composites. Atomic Force Microscopy (AFM) analysis displayed reduced surface roughness of the fabricated nanocomposite (PU/olive oil—469 nm and PU/olive oil/honey/propolis—449 nm) than the pristine PU (576 nm). The incorporation of olive oil, honey, and propolis resulted in the enhancement of the tensile strength (PU/olive oil—12.91 MPa and PU/olive oil/honey/propolis—14.346 MPa) compared with the pristine PU (7.12 MPa) as revealed in the mechanical testing. The blood clotting time of PU/olive oil (Activated partial thromboplastin time (APTT)—175 ±4 s and Partial thromboplastin time (PT)—103.3 ±3.512) was enhanced than pristine PU suggesting its improved anticoagulant behavior. Further, the developed scaffold showed low hemolytic index percentage (PU/olive oil—1.41% and PU/olive oil/honey/propolis—0.95%) than the control (2.48%) indicating its safety with RBC. Cytotoxicity test of the electrospun scaffold with human dermal fibroblast (HDF) cells using 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H tetrazolium assay demonstrated the non‐toxic and enhanced cell viability rates of HDF cells in developed scaffold than the pristine PU. Hence, the PU/olive oil/honey/propolis nanocomposite possessing better mechanical, physio‐chemical and biological properties might serve as a plausible candidate for bone tissue engineering. POLYM. COMPOS., 2018. © 2018 Society of Plastics Engineers

show abstract

Section: Resultsmentioning

confidence: 91%

Tailor‐made multicomponent electrospun polyurethane nanofibrous composite scaffold comprising olive oil, honey, and propolis for bone tissue engineering

et al. 2018

View full text Add to dashboard Cite

show abstract

“…It was observed that the intensity of the PU/carotino oil nanocomposite is decreased compared to pure PU, which clearly indicates the interaction between the PU and carotino oil as shown in Figure . The decrease in the intensity of the PU/carotino oil nanocomposites is due to the formation of the hydrogen bond . The formation of the hydrogen bond is identified by the peak shifting of CH stretch group from 2939 cm −1 in PU to 2937 cm −1 in the PU/carotino nanocomposite mats, indicating the presence of carotino oil in the PU matrix…”

Section: Resultsmentioning

confidence: 98%

Blood compatibility and physicochemical assessment of novel nanocomposite comprising polyurethane and dietary carotino oil for cardiac tissue engineering applications

Jaganathan

Mani

Manikandan

et al. 2017

J of Applied Polymer Sci

View full text Add to dashboard Cite

Cardiovascular diseases (CVD) were estimated to claim 17 million lives each year. Among these, coronary heart disease almost accounts 50% deaths associated with CVD, which causes the blockage of the coronary arteries that supplies blood to the heart. Nowadays, the cardiac tissue engineering have become a promising solution to overcome the drawbacks associated with current therapies. Further, the scaffold used in cardiac tissue engineering must possess thromboresistant and anticoagulant nature to serve as a plausible candidate for cardiovascular applications. In this present investigation, a novel nanocomposite based on polyurethane (PU) and carotino oil was fabricated using electrospinning. Scanning electron microscopy images indicated that the nanocomposites have smaller fiber diameter (702 ± 130 nm) compared to the pristine PU (969 ± 217 nm). The Fourier transform infrared spectroscopy analysis confirmed the interaction between the carotino oil and PU by the formation of hydrogen bond and shifting of CH peak. The contact angle of electrospun PU/carotino oil was found to be 119°, which was increased compared to pristine PU (86°) indicating the hydrophobic nature of developed nanocomposites. Moreover, the surface roughness and thermal stability were found to be enhanced due to the presence of carotino oil in the PU matrix indicated in atomic force microscopy and thermogravimetric analysis. The enhanced surface roughness of nanocomposites resulted in delayed activation of the blood clot as revealed in activated partial thromboplastin time and prothrombin time assay. Moreover, the hemolytic index of fabricated nanocomposites was found to very low of about 1.33% compared to pristine PU (2.73%), suggesting non‐hemolytic nature and also better blood compatibility. So, the developed PU/carotino nanocomposites having desirable characteristics like better physicochemical and blood compatibility may render appropriate potentials for raw materials of cardiac tissue engineering. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2018, 135, 45691.

show abstract

“…The band at 1456 cm −1 represents the vibrations of CH group. In spectrum of PU/GHT nanocomposites, no additional band appeared, but the characteristics peaks of PU were observed to be decreased with the blending of GHT content due to formation of hydrogen bond . The peak at 2939 cm −1 in the pure PU nanofiber corresponding to the stretching of the CH group was observed to be shifted at 2927 cm −1 in the PU/GHT composite scaffolds, indicating the presence of molecular interactions between PU and GHT in the form of hydrogen bonding .…”

Section: Resultsmentioning

confidence: 93%

Morphological, thermal, and blood‐compatible properties of electrospun nanocomposites for tissue engineering application

et al. 2017

View full text Add to dashboard Cite

In this work, the morphological, thermal, and blood compatibility properties of prepared polyurethane (PU) and gandharvahasthadi eranda thailam (GHT) nanocomposites were investigated. Morphological and thermal characterization revealed reduced diameter, improved surface roughness, and higher thermal degradation compared to control. The activated partial thromboplastin time (APTT) and prothrombin time (PT) assay revealed that the fabricated nanocomposites displayed delayed blood clotting time owing to improved surface morphology. The hemolytic assay suggested that nanocomposites would be nonhemolytic material due to low hemolytic index of about 1.15% compared to pure PU (2.733%) portraying its safety with the red blood cells. Thus, the newly developed PU/GHT composite scaffold possessing better physicochemical and blood compatibility may be considered as a potential substitute scaffold for replacing damaged tissue. POLYM. COMPOS., 39:E132–E139, 2018. © 2017 Society of Plastics Engineers

show abstract

Emu oil-based electrospun nanofibrous scaffolds for wound skin tissue engineering

Cited by 99 publications

References 14 publications

Tailor‐made multicomponent electrospun polyurethane nanofibrous composite scaffold comprising olive oil, honey, and propolis for bone tissue engineering

Tailor‐made multicomponent electrospun polyurethane nanofibrous composite scaffold comprising olive oil, honey, and propolis for bone tissue engineering

Blood compatibility and physicochemical assessment of novel nanocomposite comprising polyurethane and dietary carotino oil for cardiac tissue engineering applications

Morphological, thermal, and blood‐compatible properties of electrospun nanocomposites for tissue engineering application

Contact Info

Product

Resources

About