Emulsion-based directed evolution of enzymes and proteins in yeast

Limiting the Broadcast Range of a Secreting Cell during Intercellular Signaling Using Protease-Mediated Degradation

“…Utilizing many different and orthogonal signals generates many network connections that can be used in populations of cells comprising different strains or species. Advances have been made here in both discovering numerous pre-existing signaling molecules that can be cross-species-implemented − and designing signaling molecules using directed evolution . There are also an array of methods to modify the circuitry of individual cells, ,, thus providing further control over the network connection those cells are involved in.…”

“…Advances have been made here in both discovering numerous pre-existing signaling molecules that can be cross-speciesimplemented 25−27 and designing signaling molecules using directed evolution. 28 There are also an array of methods to modify the circuitry of individual cells, 12,29,30 thus providing further control over the network connection those cells are involved in. For example, the strength of the promoter that drives gene expression can be varied to tune protein production levels in order to change the amount of signal a cell is producing.…”