Enantiodifferential Approach for the Detection of the Target Membrane Protein of the Jasmonate Glycoside that Controls the Leaf Movement of <i>Albizzia saman</i>

Nakamura, Yoko; Miyatake, Ryoji

doi:10.1002/ange.200801820

Cited by 11 publications

(3 citation statements)

References 23 publications

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“…This strategy was successfully used for identification of nucleophosmin and Crm1 as targets of indoloquinolizines termed centrocountins (Table 1, entry 7, see also Section 5.4) 17. An enantiodifferentiating strategy for target identification was chosen also for jasmonate glucoside (Table 1, entry 8) which is a leaf‐closing factor in plants and whose enantiomer is inactive 54. A photoaffinity probe of jasmonate glucoside coupled to biotin was employed in pulldown experiments and the respective inactive enantiomer served as control.…”

Section: Approaches To Target Identificationmentioning

confidence: 99%

Target Identification for Small Bioactive Molecules: Finding the Needle in the Haystack

et al. 2013

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Identification and confirmation of bioactive small-molecule targets is a crucial, often decisive step both in academic and pharmaceutical research. Through the development and availability of several new experimental techniques, target identification is, in principle, feasible, and the number of successful examples steadily grows. However, a generic methodology that can successfully be applied in the majority of the cases has not yet been established. Herein we summarize current methods for target identification of small molecules, primarily for a chemistry audience but also the biological community, for example, the chemist or biologist attempting to identify the target of a given bioactive compound. We describe the most frequently employed experimental approaches for target identification and provide several representative examples illustrating the state-of-the-art. Among the techniques currently available, protein affinity isolation using suitable small-molecule probes (pulldown) and subsequent mass spectrometric analysis of the isolated proteins appears to be most powerful and most frequently applied. To provide guidance for rapid entry into the field and based on our own experience we propose a typical workflow for target identification, which centers on the application of chemical proteomics as the key step to generate hypotheses for potential target proteins.

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Section: Approaches To Target Identificationmentioning

confidence: 99%

Target Identification for Small Bioactive Molecules: Finding the Needle in the Haystack

et al. 2013

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“…Diese Strategie wurde erfolgreich zur Identifizierung von Nukleophosmin und Crm1 als Zielproteine der Centrocountine eingesetzt (Tabelle 1, Eintrag 7, siehe auch Abschnitt 5.4) 17. Ein enantiodifferenzierender Ansatz wurde auch bei der Zielprotein‐Identifizierung für das Jasmonat‐Glukosid gewählt (Tabelle 1, Eintrag 8), dessen Enantiomer inaktiv ist 54. Eine Photoaffinitätssonde von Jasmonat‐Glukosid wurde an Biotin gekuppelt und in Pulldown‐Experimenten verwendet, wobei das inaktive Enantiomer als Kontrolle diente.…”

Section: Ansätze Zur Identifizierung Von Zielproteinenunclassified

Identifizierung der Zielproteine bioaktiver Verbindungen: Die Suche nach der Nadel im Heuhaufen

et al. 2013

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Die Identifizierung und Validierung von Zielmolekülen biologisch aktiver niedermolekularer Verbindungen ist ein äußerst wichtiger, oft maßgebender Schritt in der akademischen und pharmazeutischen Forschung. Dank der Entwicklung und der Verfügbarkeit diverser neuer experimenteller Verfahren ist die Identifizierung von Zielmolekülen prinzipiell realisierbar, und die Anzahl der erfolgreichen Beispiele wächst stetig. Allerdings ist bislang keine generische, breit anwendbare Methodik etabliert worden. Dieser Aufsatz fasst die gängigen Methoden zur Identifizierung der Zielmoleküle niedermolekularer Substanzen in erster Linie für Chemiker, aber auch für Biologen zusammen, die z. B. den molekularen Angriffspunkt (Target) einer bestimmten biologisch aktiven Substanz finden wollen. Wir beschreiben die am häufigsten eingesetzten experimentellen Ansätze zur Zielprotein‐Identifizierung und geben repräsentative Beispiele, die den neuesten Stand der Forschung demonstrieren. Von den derzeit verfügbaren Methoden werden die Affinitätsisolierung von Proteinen mittels geeigneter Affinitätssonden (pull down) und die anschließende massenspektrometrische Analyse der angereicherten Proteine am häufigsten eingesetzt. Um eine Anleitung für einen schnellen Einstieg in dieses Feld zu geben, beschreiben wir basierend auf unseren eigenen Erfahrungen einen typischen Ablauf der Zielprotein‐Identifizierung, der auf dem Einsatz der chemischen Proteomik als Schlüsselschritt für die Formulierung einer Hypothese für potentielle Zielproteine beruht.

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“…In particular, nyctinastic leaf closing is mediated by potassium β‐ d ‐glucopyranosyl 12‐hydroxyjasmonate. In the case of nyctinasty, it is suggested that the biochemical factors accounting for leaf closing and opening directly affect K + channel activity, thereby modulating turgor pressure in specialized flexor cells [48–50].…”

Section: Physiological Processes Mediated By Opdamentioning

confidence: 99%

Plant oxylipins: Plant responses to 12‐oxo‐phytodienoic acid are governed by its specific structural and functional properties

2009

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One of the most challenging questions in modern plant science is how plants regulate their morphological and developmental adaptation in response to changes in their biotic and abiotic environment. A comprehensive elucidation of the underlying mechanisms will help shed light on the extremely efficient strategies of plants in terms of survival and propagation. In recent years, a number of environmental stress conditions have been described as being mediated by signaling molecules of the oxylipin family. In this context, jasmonic acid, its biosynthetic precursor, 12‐oxo‐phytodienoic acid (OPDA), and also reactive electrophilic species such as phytoprostanes play pivotal roles. Although our understanding of jasmonic acid‐dependent processes and jasmonic acid signal‐transduction cascades has made considerable progress in recent years, knowledge of the regulation and mode of action of OPDA‐dependent plant responses is just emerging. This minireview focuses on recent work concerned with the elucidation of OPDA‐specific processes in plants. In this context, aspects such as the differential recruitment of OPDA, either by de novo biosynthesis or by release from cyclo‐oxylipin‐galactolipids, and the conjugation of free OPDA are discussed.

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Enantiodifferential Approach for the Detection of the Target Membrane Protein of the Jasmonate Glycoside that Controls the Leaf Movement of Albizzia saman

Cited by 11 publications

References 23 publications

Target Identification for Small Bioactive Molecules: Finding the Needle in the Haystack

Target Identification for Small Bioactive Molecules: Finding the Needle in the Haystack

Identifizierung der Zielproteine bioaktiver Verbindungen: Die Suche nach der Nadel im Heuhaufen

Plant oxylipins: Plant responses to 12‐oxo‐phytodienoic acid are governed by its specific structural and functional properties

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