ENANTIOMERIC SEPARATION OF AMINOGLUTETHIMIDE, ACETYL AMINOGLUTETHIMIDE, AND DANSYL AMINOGLUTETHIMIDE BY TLC WITH Β-Cyclodextrin AND DERIVATIVES AS MOBILE PHASE ADDITIVES

Abstract

Racemic aminoglutethimide and acetylaminoglutethimide were resolved on a fluorescent Silica gel 60A TLC plate using 30% hydroxy trimethylpropylammonium-β-cyclodextrin with methanol (50:50v/v) as the mobile phase. Also, the resolution of the enantiomers of aminoglutethimide as dansyl derivatives were investigated on reversed phase TLC using a mobile phase consist-2715 Downloaded by [University of Otago] at 08:24 02 October 2015ing of a β-cyclodextrin solution (0.05 M saturated with urea), or 15% (carboxymethyl-β-cyclodextrin) solution with methanol (65:35v/v). Enantiomeric resolution was found to be highly dependent on mobile phase composition. The effect of the type and amount of organic modifier as well as the concentration of β-CD derivative, and the pH of the mobile phase on resolution were studied. Three types of maltodextrins failed to show resolution for any of the racemic compounds studied.

Analysis of anticancer drugs using thin layer chromatography- A review

“…Experimental procedures of this method are given in Table 1. Mobile phase composition was found to be very important for enantiomeric resolution [38].…”