Enantioselective analysis of amisulpride in pharmaceutical formulations by means of capillary electrophoresis

Musenga, Alessandro; Mandrioli, Roberto; Morganti, Emanuele; Fanali, Salvatore; Raggi, Maria Augusta

doi:10.1016/j.jpba.2007.05.022

Cited by 18 publications

(9 citation statements)

References 22 publications

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“…These CD derivatives are charged throughout the complete pH range. Recent applications with sulfated-β-CD have been described [33][34][35][36]. An enantioselective method was developed for the analysis of amisulpiride enantiomers in tablets [33].…”

Section: Cyclodextrins As Chiral Selectorsmentioning

confidence: 99%

“…Recent applications with sulfated-β-CD have been described [33][34][35][36]. An enantioselective method was developed for the analysis of amisulpiride enantiomers in tablets [33]. After investigation of the effects of CD type and concentration, temperature, buffer concentration, and pH, baseline separation of the enantiomers was obtained within 10 min.…”

Section: Cyclodextrins As Chiral Selectorsmentioning

confidence: 99%

See 1 more Smart Citation

Chiral Separations by Capillary Electrophoresis

Mangelings

Heyden

2010

Chiral Separation Methods for Pharmaceutical and Biotechnological Products

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Section: Cyclodextrins As Chiral Selectorsmentioning

confidence: 99%

Section: Cyclodextrins As Chiral Selectorsmentioning

confidence: 99%

Chiral Separations by Capillary Electrophoresis

Mangelings

Heyden

2010

Chiral Separation Methods for Pharmaceutical and Biotechnological Products

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“…These include gas chromatography (GC) (8,9), spectrofluorimetric techniques (10,11), high-performance liquid chromatographic (HPLC) methods with UV detection (12)(13)(14), HPLC with fluorescence detection (15,16), capillary electrophoresis (17,18) and adsorptive stripping voltammetry (19). These include gas chromatography (GC) (8,9), spectrofluorimetric techniques (10,11), high-performance liquid chromatographic (HPLC) methods with UV detection (12)(13)(14), HPLC with fluorescence detection (15,16), capillary electrophoresis (17,18) and adsorptive stripping voltammetry (19).…”

Section: Introductionmentioning

confidence: 99%

Determination of sulpiride in pharmaceutical preparations and biological fluids using a Cr (III) enhanced chemiluminescence method

et al. 2012

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A highly sensitive and simple method for identifying sulpiride in pharmaceutical formulations and biological fluids is presented. The method is based on increased chemiluminescence (CL) intensity of a luminol-H2O2 system in response to the addition of Cr (III) under alkaline conditions. The CL intensity of the luminol-H2O2-Cr (III) system was greatly enhanced by the addition of sulpiride and the CL intensity was proportional to the concentration of sulpiride in a sample solution. Various parameters affecting the CL intensity were systematically investigated and optimized for determination of the sulpiride in a sample. Under the optimum conditions, the CL intensity was proportional to the concentration of sulpiride in the range of 0.068-4.0 µg/mL, with a good correlation coefficient of 0.997. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 8.50 × 10(-6) µg/mL and 2.83 × 10(-5) µg/mL, respectively. The method presented here produced good reproducibility with a relative standard deviation (RSD) of 2.70% (n = 7). The effects of common excipients and metal ions were studied for their interference effect. The method was validated statistically through recovery studies and successfully applied for the determination of sulpiride in pure form, pharmaceutical preparations and spiked human plasma samples. The percentage recoveries were found to range from 99.10 to 100.05% for pure form, 98.12 to 100.18% for pharmaceutical preparations and 97.9 to 101.4% for spiked human plasma.

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“…These methods include gas chromatography (GC) (9,10), spectrophotometry (11,12), high-performance liquid chromatography (HPLC) with UV detection (13)(14)(15), HPLC with fluorescence detection (16,17), HPLC-mass spectrometry (MS) (18), GC-MS (19), capillary electrophoresis (20,21) and adsorptive stripping voltammetry (22). These methods include gas chromatography (GC) (9,10), spectrophotometry (11,12), high-performance liquid chromatography (HPLC) with UV detection (13)(14)(15), HPLC with fluorescence detection (16,17), HPLC-mass spectrometry (MS) (18), GC-MS (19), capillary electrophoresis (20,21) and adsorptive stripping voltammetry (22).…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric method for the determination of sulpiride in pharmaceutical preparations and human plasma through derivatization with 2‐cyanoacetamide

et al. 2012

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A sensitive and accurate spectrofluorimetric method has been developed for the determination of sulpiride in pharmaceutical preparations and human plasma. The developed method is based on the derivatization reaction of 2-cyanoacetamide with sulpiride in 30% ammonical solution. The fluorescent derivatized reaction product exhibited maximum fluorescence intensity at 379 nm after excitation at 330 nm. The optimum conditions for derivatization reactions were studied and the fluorescence intensity versus concentration plot was found to be linear over the concentration range 0.2-20.0 µg/mL with a correlation coefficient of 0.9985. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 0.82 and 2.73 ng/mL, respectively. The proposed method was validated according to ICH guidelines. The effects of common excipients and co-administered drugs were also studied. The accuracy of the method was checked using the standard addition method and percent recoveries were found to be in the range of 99.00-101.25% for pharmaceutical preparations and 97.00-97.80% for spiked human plasma. The method was successfully applied to commercial formulations and the results obtained for the proposed method were compared with a high-performance liquid chromatography reference method and statistically evaluated using the Student's t-test for accuracy and the variance ratio F-test for precision. A reaction pathway was also proposed.

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Enantioselective analysis of amisulpride in pharmaceutical formulations by means of capillary electrophoresis

Cited by 18 publications

References 22 publications

Chiral Separations by Capillary Electrophoresis

Chiral Separations by Capillary Electrophoresis

Determination of sulpiride in pharmaceutical preparations and biological fluids using a Cr (III) enhanced chemiluminescence method

Spectrofluorimetric method for the determination of sulpiride in pharmaceutical preparations and human plasma through derivatization with 2‐cyanoacetamide

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