Enantioselective Supercritical Fluid Chromatography–Tandem Mass Spectrometry Method for Simultaneous Estimation of Risperidone and its 9-Hydroxyl Metabolites in Rat Plasma

Prasad, Thatipamula Rajendra; Joseph, Siji; Kole, Prashant; Kumar, Anoop; Subramanian, Murali; Ramasamy, Sudha; Kr, Prabhakar

doi:10.4155/bio-2017-0168

Cited by 12 publications

(1 citation statement)

References 37 publications

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“…In addition, to assess whether LGG administration disturbed the TDF levels in blood, the serum TDF concentrations in the five groups were detected using an Agilent 1260 HPLC instrument (Agilent Technologies, Santa Clara, CA, USA) as previously described. 28 Samples were measured at least in duplicate.…”

Section: Assay For Biochemical Markersmentioning

confidence: 99%

Lactobacillus rhamnosus GG attenuates tenofovir disoproxil fumarate-induced bone loss in male mice via gut-microbiota-dependent anti-inflammation

Líu

et al. 2019

Therapeutic Advances in Chronic Disease

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Background: Although antiretroviral agents trigger bone loss in human immunodeficiency virus patients, tenofovir disoproxil fumarate (TDF) induces more severe bone damage, such as osteoporosis. While, the mechanisms are unclear, probiotic supplements may be effective against osteoporosis. Methods: C57BL6/J mice were administered with Lactobacillus rhamnosus GG (LGG)+TDF, TDF, and zoledronic acid+TDF, respectively. Bone morphometry and biomechanics were evaluated using microcomputed tomography, bone slicing, and flexural tests. The lymphocyte, proinflammatory cytokines, and intestinal permeability levels were detected using enzyme-linked immunosorbent assays, quantitative real-time polymerase chain reaction, and flow cytometry. The gut microbiota composition and metabolomics were analyzed using 16S recombinant deoxyribonucleic acid pyrosequencing and ultra-performance liquid-chromatography–quadrupole time-of-flight mass spectrometry. Results: LGG administered orally induced marked increases in trabecular bone microarchitecture, cortical bone volume, and biomechanical properties in the LGG+TDF group compared with that in the TDF-only group. Moreover, LGG treatment increased intestinal barrier integrity, expanded regulatory T cells, decreased Th17 cells, and downregulated osteoclastogenesis-related cytokines in the bone marrow, spleen, and gut. Furthermore, LGG reconstructed the gut microbiota and changed the metabolite composition, especially lysophosphatidylcholine levels. However, the amount of N-acetyl-leukotriene E4 was the highest in the TDF-only group. Conclusion: LGG reconstructed the community structure of the gut microbiota, promoted the expression of lysophosphatidylcholines, and improved intestinal integrity to suppress the TDF-induced inflammatory response, which resulted in attenuation of TDF-induced bone loss in mice. LGG probiotics may be a safe and effective strategy to prevent and treat TDF-induced osteoporosis.

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Section: Assay For Biochemical Markersmentioning

confidence: 99%

Lactobacillus rhamnosus GG attenuates tenofovir disoproxil fumarate-induced bone loss in male mice via gut-microbiota-dependent anti-inflammation

Líu

et al. 2019

Therapeutic Advances in Chronic Disease

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Matrix effect in bioanalytical assay development using supercritical fluid chromatography‐mass spectrometry

Chen,

Cui,

Dean

et al. 2023

Biomedical Chromatography

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Matrix effect (ME) is commonly caused by coelution of compounds with target analytes, resulting in either suppression or enhancement of analyte ionization. Thus, to achieve the desired accuracy, precision, and sensitivity, ME needs to be evaluated and controlled during bioanalytical method development. As the application of supercritical fluid chromatography‐mass spectrometry (SFC‐MS) for analysis of biological samples has increased, ME using SFC‐MS has also been investigated with a focus on the difference in ME in SFC‐MS compared to other chromatographic techniques used for achiral separation in biological samples. Here, we provide a summary of the status of ME evaluation and mitigation in SFC‐MS methods. This review presents an overview of the phenomenon of ME and methods for evaluating ME in bioanalysis. Next, the factors that can impact ME in SFC‐MS‐based bioanalytical methods are discussed in detail with an emphasis on SFC. A literature review of the evaluation of ME in targeted bioanalytical methods using SFC‐MS is included at the end. Robust instrumentation, effective sample preparation, and superb separation selectivity are the foundations of reliable analytical methods as well as the ability to mitigate detrimental ME in SFC‐MS methods.

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Supercritical Fluid Technologies: A Green Solvent Approach for Pharmaceutical Product Development

Pattnaik

Arun

Swain

2020

Nanotechnology in the Life Sciences

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Enantioselective Supercritical Fluid Chromatography–Tandem Mass Spectrometry Method for Simultaneous Estimation of Risperidone and its 9-Hydroxyl Metabolites in Rat Plasma

Abstract: The method was validated and was successfully applied for simultaneous estimation of RIS and 9-hydroxyrisperidone metabolites (R & S individually) after intravenous and per oral administration to rats.

Cited by 12 publications

References 37 publications

Lactobacillus rhamnosus GG attenuates tenofovir disoproxil fumarate-induced bone loss in male mice via gut-microbiota-dependent anti-inflammation

Lactobacillus rhamnosus GG attenuates tenofovir disoproxil fumarate-induced bone loss in male mice via gut-microbiota-dependent anti-inflammation

Matrix effect in bioanalytical assay development using supercritical fluid chromatography‐mass spectrometry

Supercritical Fluid Technologies: A Green Solvent Approach for Pharmaceutical Product Development

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