Enantioselective synthesis of S-(−)-1-phenylethanol in Candida utilis semi-fed-batch cultures

Yeast‐mediated enantioselective synthesis of chiral R‐(+)‐ and S‐(−)‐1‐phenyl‐1‐butanol from prochiral phenyl n‐propyl ketone in hexane–water biphasic culture

“…The biphasic cell cultures were then put in an orbital shaking incubator at a temperature of 30 °C and a shaking rate of 150 rpm for a period of 7 days. The external addition of β‐NADH was found helpful in this study and was supported by comparison with results of experiments performed without adding the β‐NADH externally and with our previous research 23…”

“…In this paper, based on the successful reduction of 2‐butanone and acetophenone with a strain of Candida utilis yeast in aqueous culture,23, 24 a water‐insoluble model compound, phenyl n ‐propyl ketone, is utilized to test the enantioselective reduction to chiral 1‐phenyl‐1‐butanol in either aqueous culture or hexane–water biphasic culture with Saccharomyces cerevisiae and Candida utilis yeasts. The effect of volume percentage of n ‐hexane and amount of cofactor Zn 2+ ion in the biphasic culture on variation of cell growth and catalytic activity of the two strains was studied.…”

Column Switching Technique, Assisted On‐Line Matrix Elimination, and Chiral Analysis of R‐ and S‐1‐Phenyl‐1‐butanol in Biphasic Saccharomyces cerevisiae Mediated Culture

“…Today, the commercially available derivatized polysaccharide stationary phase columns, Chiralcel OB and Chiralpak AD, developed for HPLC, have led to many applications in the agrochemical, food, and drink industry and, especially, in the pharmaceutical industry. [1][2][3][4] However, the sample pretreatment for eliminating matrix interference or for enriching the concentration of analytes cannot be performed together with the separation and, sometimes, tedious procedures of the sample pretreatment should be followed in these applications.…”

Enhancement of asymmetric bioreduction of ethyl 4-chloro acetoacetate by the design of composition of culture medium and reaction conditions

“…This chiral synthon can be produced by the asymmetric reduction of ethyl 4-chloro-3-oxobutyrate with bakers' yeast. www.elsevier.com/locate/procbio Process Biochemistry 42 (2007) [1][2][3][4][5][6][7] In such systems, the optical purity of the product and the reaction yield are general the two indexes of reaction performance [14,22].…”