Enantioseparation of d,l-2-hydroxyglutaric acid by capillary electrophoresis with tandem mass spectrometry—Fast and efficient tool for d- and l-2-hydroxyglutaracidurias diagnosis

Švidrnoch, Martin; Přibylka, Adam; Bekárek, Vojtěch; Ševčı́k, Juraj; Smolka, Vratislav; Maier, Vítězslav

doi:10.1016/j.chroma.2016.05.095

Cited by 19 publications

(24 citation statements)

References 36 publications

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“…Urinary peptidomics by CE‐MS was furthermore used for the identification of biomarkers for chronic obstructive pulmonary disease , diastolic left ventricular dysfunction , end‐stage renal disease , chronic graft‐versus‐host disease , non‐alcoholic fatty liver disease and systemic lupus erythematosus . A chiral CE‐MS method was published by Švidrnoch to determine D/L‐2‐hydroxyglutaric acid without derivatization in urine as biomarker for a rare neurometabolic disorder .…”

Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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Capillary electrophoresis (CE) offers fast and high‐resolution separation of charged analytes from small injection volumes. Coupled to mass spectrometry (MS), it represents a powerful analytical technique providing (exact) mass information and enables molecular characterization based on fragmentation. Although hyphenation of CE and MS is not straightforward, much emphasis has been placed on enabling efficient ionization and user‐friendly coupling. Though several interfaces are now commercially available, research on more efficient and robust interfacing with nano‐electrospray ionization (ESI), matrix‐assisted laser desorption/ionization (MALDI) and inductively coupled plasma mass spectrometry (ICP) continues with considerable results. At the same time, CE‐MS has been used in many fields, predominantly for the analysis of proteins, peptides and metabolites. This review belongs to a series of regularly published articles, summarizing 248 articles covering the time between June 2016 and May 2018. Latest developments on hyphenation of CE with MS as well as instrumental developments such as two‐dimensional separation systems with MS detection are mentioned. Furthermore, applications of various CE‐modes including capillary zone electrophoresis (CZE), nonaqueous capillary electrophoresis (NACE), capillary gel electrophoresis (CGE) and capillary isoelectric focusing (CIEF) coupled to MS in biological, pharmaceutical and environmental research are summarized.

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Section: Analytical Applicationsmentioning

confidence: 99%

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

et al. 2018

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“…There were 12 papers on in‐capillary derivatisation . The derivatisation was performed by adding the reagents to the separation buffer or by zone injections of reagents and sample.…”

Section: Derivatisation Modesmentioning

confidence: 99%

“…Derivatisation was used to improve the separation of naphthenic acids , amino acids , metabolite , proteins , racemic drugs , and pharmaceutical impurities . To enable characterisation of individual compounds of a complex mixture, MS detection can be essential, however, it requires sufficient separation prior to detection.…”

Section: Derivatisation For Separationmentioning

confidence: 99%

“…Instead of enantiomeric off‐line derivatisation, Švidrnocha et al. separated 2‐hydroxyglutaric acid enantiomers and enantiomeric metabolites using vancomycin inclusion complexes in EKC‐MS/MS . The method enabled separation of the D ‐ or L ‐enantiomer in less than 10 min by simply adding the chiral reagent to the separation buffer.…”

Section: Derivatisation For Separationmentioning

confidence: 99%

“…This is often the case for fluorescence detection where native fluorescence is rather uncommon and only the labelled species provide a strong signal. There were 24 articles on LIF , 16 on UV , 3 on electrochemical detection , and 4 on MS . In MS detection, derivatisation was used to improve the separation (see Section ) and to enable MS detection in favourable positive ion ESI mode .…”

Section: Derivatisation For Detectionmentioning

confidence: 99%

See 2 more Smart Citations

Derivatisation for separation and detection in capillary electrophoresis (2015–2017)

Wuethrich

Quirino

2017

Electrophoresis

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Derivatisation is an integrated part of many analytical workflows to enable separation and detection of the analytes. In CE, derivatisation is adapted in the four modes of pre-capillary, in-line, in-capillary, and post-capillary derivatisation. In this review, we discuss the progress in derivatisation from February 2015 to May 2017 from multiple points of view including sections about the derivatisation modes, derivatisation to improve the analyte separation and analyte detection. The advancements in derivatisation procedures, novel reagents, and applications are covered. A table summarising the 46 reviewed articles with information about analyte, sample, derivatisation route, CE method and method sensitivity is provided.

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Chiral Separation by Capillary Electrophoresis and Capillary Electrophoresis–Mass Spectrometry

Clark¹,

Somsen²,

Köhler³

2023

Chiral Separations and Stereochemical Elucidation

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Enantioseparation of d,l-2-hydroxyglutaric acid by capillary electrophoresis with tandem mass spectrometry—Fast and efficient tool for d- and l-2-hydroxyglutaracidurias diagnosis

Cited by 19 publications

References 36 publications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Recent advances in capillary electrophoresis‐mass spectrometry: Instrumentation, methodology and applications

Derivatisation for separation and detection in capillary electrophoresis (2015–2017)

Chiral Separation by Capillary Electrophoresis and Capillary Electrophoresis–Mass Spectrometry

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