Encapsulated <i>Staphylococcus aureus</i> strains vary in adhesiveness assessed by atomic force microscopy

Coldren, Faith; Palavecino, Elizabeth; Levi‐Polyachenko, Nicole; Smith, Thomas L.; Smith, Beth Paterson; Webb, Lawrence X.; Carroll, David

doi:10.1002/jbm.a.31973

Cited by 12 publications

(9 citation statements)

References 39 publications

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“…Interestingly, previous research suggests that although S. aureus strain 8325-4 carries a serotype-5 capsule gene, it is defective in capsule expression 31,32 . However, Coldren and colleagues found very similar morphological characteristics in a serotype-8 capsulepositive S. aureus strain when imaged with AFM under comparable conditions 33 .…”

Section: Discussionmentioning

confidence: 93%

Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

et al. 2016

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Abstract:Despite the well-known beneficial effects of biomaterial nanopatterning on host tissue integration, the influence of controlled nanoscale topography on bacterial colonisation and infection remains unknown. Therefore, the aim of the present study was to determine the nanoscale effect of surface nanopatterning on biomaterial colonisation by S. aureus, utilising AFM nanomechanics and single-cell force spectroscopy (SCFS). Nanoindentation of S. aureus bound to planar (PL) and nanopatterned (SQ) polycarbonate (PC) surfaces suggested two distinct areas of mechanical properties, consistent with a central bacterial cell surrounded by a capsullar component. Nevertheless, no differences in elastic moduli were found between bacteria bound to PL and SQ, suggesting a minor role of nanopatterning in bacterial cell elasticity. Furthermore, SCFS demonstrated increased adhesion forces and work between S. aureus and SQ surfaces at 0s and 1s contact times. Although WLC modelling showed similarities in contour lengths for attachment to both surfaces, Poisson analysis suggests increased short-range forces for the S. aureus-SQ interactions. In the case of S. aureus-PL, long-range forces were found to not only be dominant but also repulsive in nature, which may help explain the reduced adhesion forces observed during AFM probing. In conclusion, although surface nanopatterning does not significantly influence the elasticity of attached bacterial cells, it was found to promote the early-adhesion of S. aureus cells to the biomaterial surface.

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Section: Discussionmentioning

confidence: 93%

Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

et al. 2016

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“…The binding forces that initiate a biofilm have been investigated for a handful of typestrains or laboratory-derived strains of S. aureus (e.g., refs. [16][17][18][19][20] and related species such as Staphylococcus epidermidis (e.g., ref. 21).…”

Section: Resultsmentioning

confidence: 99%

Polymorphisms in fibronectin binding protein A of Staphylococcus aureus are associated with infection of cardiovascular devices

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Casillas-Ituarte

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

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Medical implants, like cardiovascular devices, improve the quality of life for countless individuals but may become infected with bacteria like Staphylococcus aureus. Such infections take the form of a biofilm, a structured community of bacterial cells adherent to the surface of a solid substrate. Every biofilm begins with an attractive force or bond between bacterium and substratum. We used atomic force microscopy to probe experimentally forces between a fibronectin-coated surface (i.e., proxy for an implanted cardiac device) and fibronectin-binding receptors on the surface of individual living bacteria from each of 80 clinical isolates of S. aureus. These isolates originated from humans with infected cardiac devices (CDI; n = 26), uninfected cardiac devices (n = 20), and the anterior nares of asymptomatic subjects (n = 34). CDI isolates exhibited a distinct bindingforce signature and had specific single amino acid polymorphisms in fibronectin-binding protein A corresponding to E652D, H782Q, and K786N. In silico molecular dynamics simulations demonstrate that residues D652, Q782, and N786 in fibronectin-binding protein A form extra hydrogen bonds with fibronectin, complementing the higher binding force and energy measured by atomic force microscopy for the CDI isolates. This study is significant, because it links pathogenic bacteria biofilms from the length scale of bonds acting across a nanometer-scale space to the clinical presentation of disease at the human dimension.adhesion | binding strength | host-pathogen interaction | pacemaker | prosthesis

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“…From published literature, S. aureus is softer than E. coli and the polysaccharide coating varies in its \stickiness" depending on the bacterial serotype. 39,40 Electrically Conductive Polymer Nanotubes with Anti-Bacterial Properties…”

Section: Resultsmentioning

confidence: 99%

Electrically Conductive Polymer Nanotubes With Anti-Bacterial Properties

et al. 2012

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Nanotubes (NT) composed of the electrically active polymer poly (3,4-ethylenedioxythiophene) (PEDOT) have been used for photothermal ablation of both gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli) bacteria. Since infrared absorption of PEDOT is dominated by bipolarons strongly coupled to phonons, we hypothesize that nonradiative decay of these states leads to heat generation. Photothermal death of bacteria by PEDOT NT was compared to single-wall carbon nanotubes (SWNT). Complete eradication of bacterial colonies incubated with 100 ug/ml of either PEDOT NT or SWNT occurred with a single exposure to 1064 nm light (3.8 W/cm2) for 60 s. PEDOT NT were also shown to elicit a mild antibacterial response upon incubation with bacteria and no infrared exposure. PEDOT NT have the same capacity for photothermal ablation of bacteria as compared to SWNT; therefore, they represent an exciting new class of polymer based nanoparticles for medically-relevant photothermal therapies.

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Encapsulated Staphylococcus aureus strains vary in adhesiveness assessed by atomic force microscopy

Cited by 12 publications

References 39 publications

Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

Influence of biomaterial nanotopography on the adhesive and elastic properties of Staphylococcus aureus cells

Polymorphisms in fibronectin binding protein A of Staphylococcus aureus are associated with infection of cardiovascular devices

Electrically Conductive Polymer Nanotubes With Anti-Bacterial Properties

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