Encapsulation and Growth Characteristics of Three Different Cells in Alginate Gel Beads Containing Reconstituted Collagen Fibers

Tsai, Shiao-Wen; Hsu, Fu‐Yin; Wang, Yng Jiin

doi:10.4015/s1016237206000129

Cited by 3 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, to the best of our knowledge, few studies have reported that cells can be positioned during the fabrication of 3D scaffolds with a specific fibril orientation. We have reported several methods for producing collagen gel beads containing cells, such as a collagen/alginate system [30,31,34], a pneumatically driven microfluidic system [37], and a pneumatically driven external vibration system [36]. The collagen fibril orientation in the former two systems was not well-controlled.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, preserving D-banding during manufacturing is important for creating a biomimetic microenvironment for cell attachment, differentiation and organization in vitro. In our previous studies, we developed several methods to produce a reconstituted collagen fibril matrix as a cell growth substrate through the aid of alginate [30][31][32] or chondroitin sulfate [33] or combined with other biomaterials such as hydroxyapatite [34] and hyaluronic acid [35]. However, the collagen fibrils within these systems are randomly distributed.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Construction of cell-containing, anisotropic, three-dimensional collagen fibril scaffolds using external vibration and their influence on smooth muscle cell phenotype modulation

Zeng¹,

Kang²,

Rau³

et al. 2017

Biomed. Mater.

Self Cite

View full text Add to dashboard Cite

Numerous methods have been developed for preparing guiding channels/tracks to promote the alignment of highly oriented cell types. However, these manufacture methods cannot fabricate interconnected guiding channels within three-dimensional (3D) scaffolds. Providing a suitable architectural scaffold for cell attachment could lead cells to more rapidly display a desired phenotype and perform their unique functions. Previously, we developed a simple device composed of a pneumatic membrane that can generate a tunable vibration frequency to apply physical stimulation for fabricating a 3D aligned collagen fibril matrix with the characteristic D-period structure in one step. In the present study, we aimed to evaluate the cellular responses of thoracic aortic smooth muscle cells (A7r5) incorporated during the fabrication of 3D-aligned collagen fibrils with D-periods and compared these cells with those incorporated in a 3D, randomly distributed collagen matrix and in a two-dimensional (2D) aligned substrate after up to 10 days of culture. The results consistently demonstrated that A7r5 cells cultured within the 3D and 2D anisotropic matrices were aligned. Cells cultured in the 3D aligned scaffolds exhibited a higher proliferation rate as well as higher F-actin and smoothelin expression levels compared with cells cultured in 3D randomly distributed scaffolds. Together, these results indicate that a 3D-reconstituted, anisotropic collagen matrix fabricated by our process provides synergistic effects of tension stimulation and matrix stiffness on encapsulated cells and can direct A7r5 cells to transform from a synthetic phenotype into a contractile state.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Construction of cell-containing, anisotropic, three-dimensional collagen fibril scaffolds using external vibration and their influence on smooth muscle cell phenotype modulation

Zeng¹,

Kang²,

Rau³

et al. 2017

Biomed. Mater.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The 40% loss of cell vitality could be attributed to the osmotic shock or the intermittent changes of cell environment such as high calcium concentration during gel bead formation and various exposures to different buffers. The 60% cell survival rate obtained using this entrapment procedure is considered high as compared with the conventional procedure; about 10% survival rate was found with the alginate microspheres on chondrocytes and osteoblasts (McConell et al, 2004;Tsai et al, 2006).…”

Section: Growth Of C2c12 Cells Entrapped Within the Collagen Microsphmentioning

confidence: 99%

“…We found that this encapsulation system is suitable for culturing GH3 cells (a tumor cell line derived from pituitary tumor) which can be cultivated in both attached and suspended modes. However, adherent cells such as fibroblasts and osteoblasts did not grow well when entrapped inside these microcapsules (Tsai et al, 2006). Apparently, the entrapped alginate inside the microcapsules needs to be removed for better cell attachment and proliferation.…”

Section: Introductionmentioning

confidence: 99%

A novel method of encapsulating and cultivating adherent mammalian cells within collagen microcarriers

Wu¹,

Huang²,

Hsu³

et al. 2007

Biotech & Bioengineering

Self Cite

View full text Add to dashboard Cite

A novel method of preparing collagen microcarriers was developed and used to entrap adherent cells for cell culturing. This new technique involved seeding of cells in micro gel beads comprised of collagen fibrils dispersed in alginate. The gel beads were washed with phosphate buffered saline (PBS) to remove alginate and the resulting microspheres, about 300-500 microm in diameter, contained evenly distributed collagen fibrils which provided a 3D biomimetic environment for cell growth. The applicability of this microencapsulating system was demonstrated by its ability to support the growth of C2C12 myoblast cells. When seeded and cultured within the 3D collagen microcarriers, the population of C2C12 cells entrapped within the microcarriers increased by 1.5 folds in 7 days after inoculation. This encapsulation technique is potentially useful for culturing cells and especially useful for adherent cells that require a 3D fibrillar collagen environment.

show abstract

Alternative approach of cell encapsulation by Volvox spheres

Teong

Manousakas

Chang

et al. 2015

Materials Science and Engineering: C

View full text Add to dashboard Cite

Encapsulation and Growth Characteristics of Three Different Cells in Alginate Gel Beads Containing Reconstituted Collagen Fibers

Cited by 3 publications

References 6 publications

Construction of cell-containing, anisotropic, three-dimensional collagen fibril scaffolds using external vibration and their influence on smooth muscle cell phenotype modulation

Construction of cell-containing, anisotropic, three-dimensional collagen fibril scaffolds using external vibration and their influence on smooth muscle cell phenotype modulation

A novel method of encapsulating and cultivating adherent mammalian cells within collagen microcarriers

Alternative approach of cell encapsulation by Volvox spheres

Contact Info

Product

Resources

About