Encapsulation of crocetin into poly (lactic-co-glycolic acid) nanoparticles overcomes drug resistance in human ovarian cisplatin-resistant carcinoma cell line (A2780-RCIS)

Neyshaburinezhad, Navid; Kalalinia, Fatemeh; Hashemi, Maryam

doi:10.1007/s11033-019-05098-7

Cited by 12 publications

(8 citation statements)

References 33 publications

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“…In order to explore potential of this compound to treat this kind of tumor in more detail, we reported results regarding in vivo and in vitro models of glioma treated with crocetin. Several authors demonstrated the in vitro antiproliferative action of crocetin in different kinds of tumor cells [29][30][31][32]. In order to evaluate the antiproliferative effect of crocetin on GBM cells, four tumor lines (U251, U87, U138, and U373) were treated with 250 µM and 500 µM of crocetin and counted after 72 h of incubation.…”

Section: Discussionmentioning

confidence: 99%

Crocetin Extracted from Saffron Shows Antitumor Effects in Models of Human Glioblastoma

Colapietro

Mancini

Vitale

et al. 2020

IJMS

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Over recent years, many authors discussed the effects of different natural compounds on glioblastoma (GBM). Due to its capacity to impair survival and progression of different cancer types, saffron extract (SE), named crocetin (CCT), is particularly noteworthy. In this work, we elucidated the antitumor properties of crocetin in glioma in vivo and in vitro models for the first time. The in vitro results showed that the four tumor cell lines observed in this study (U251, U87, U138, and U373), which were treated with increasing doses of crocetin, showed antiproliferative and pro-differentiative effects as demonstrated by a significant reduction in the number of viable cells, deep changes in cell morphology, and the modulation of mesenchymal and neuronal markers. Indeed, crocetin decreased the expression of Cluster of Differentiation CD44, CD90, CXCR4, and OCT3/4 mesenchymal markers, but increased the expression of βIII-Tubulin and neurofilaments (NFH) neuronal linage-related markers. Epigenetic mechanisms may modulate these changes, since Histone Deacetylase, HDAC1 and HDAC3 were downmodulated in U251 and U87 cells, whereas HDAC1 expression was downmodulated in U138 and U373 cells. Western blotting analyses of Fatty Acid Synthase, FASN, and CD44 resulted in effective inhibition of these markers after CCT treatment, which was associated with important activation of the apoptosis program and reduced glioma cell movement and wound repair. The in vivo studies aligned with the results obtained in vitro. Indeed, crocetin was demonstrated to inhibit the growth of U251 and U87 cells that were subcutaneously injected into animal models. In particular, the Tumor To Progression or TTP values and Kaplan–Meier curves indicated that crocetin had more major effects than radiotherapy alone, but similar effects to temozolomide (TMZ). An intra-brain cell inoculation of a small number of luciferase-transfected U251 cells provided a model that was able to recapitulate recurrence after surgical tumor removal. The results obtained from the orthotopic intra-brain model indicated that CCT treatment increased the disease-free survival (DFS) and overall survival (OS) rates, inducing a delay in appearance of a detectable bioluminescent lesion. CCT showed greater efficacy than Radio Therapy (RT) but comparable efficacy to temozolomide in xenograft models. Therefore, we aimed to continue the study of crocetin’s effects in glioma disease, focusing our attention on the radiosensitizing properties of the natural compound and highlighting the ways in which this was realized.

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Section: Discussionmentioning

confidence: 99%

Crocetin Extracted from Saffron Shows Antitumor Effects in Models of Human Glioblastoma

Colapietro

Mancini

Vitale

et al. 2020

IJMS

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“…In addition, it has been determined that it has not toxic effects on L929 healthy cells [ 78 ]. In a study by Neshaburinezad et al [ 80 ] poly (Lactic-co-glycolic acid) -crocetin nanoparticles (PLGA-Crt NP) have been evaluated MRP1 and MRP2 activity in human ovarian cisplatin-resistant carcinoma cell line (A2780-RCIS) and parent form (A2780-RCIS). Encapsulation of crocetin PLGA NPs has enhanced the inhibitory effects on drug resistance by down-regulating MRP2 carriers.…”

Section: Main Textmentioning

confidence: 99%

The nanocomposites designs of phytomolecules from medicinal and aromatic plants: promising anticancer-antiviral applications

Kaplan

2022

Beni-Suef Univ J Basic Appl Sci

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Background Nowadays, researchers are moving toward a herbal approach to cancer treatment because of the harmful effects of synthetic anti-tumor drugs. The evaluation of active compounds with plant origin may help in the remedy of human illnesses in the future. These active compounds have direct or indirect curative efficacies on difficult to cure diseases such as cancer. Investigation of nanoforms of these active compounds is one of the curious topics of the scientific community. Main body Saffron and its components obtained from Crocus sativa, essential oils obtained from lavender, Syzygium aromaticum called cloves and Beta vulgaris are known for their anticancer effects. Nano-drugs are designed to increase the anticancer activity of plant-derived drugs. Herbal extracts operate very great in the production of nanoparticles. The aim is to ensure that only the nano-drug is delivered to the tumor site. Furthermore, nanoparticles have hazardous effects when analyzed at elevated doses, but this issue can be doped together with plant extracts. Short conclusions The nanocomposites (graphene oxide, solid lipid nano and nanoemulsion) of phytomolecules obtained from saffron, clove, lavender and red beet may be effective in minimizing these toxic effects. In the near future, detecting the anticancer molecular mechanisms of these naturally derived compounds and nanocomposites could contribute to further cancer research. Apart from these, these compounds and its nanocomposites could have antiviral effects against today's threat covid-19 virus. Consequently, more promising anticancer and antiviral agents would be discovered. Graphical abstract

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“…Neyshaburinezhad et al encapsulated crocetin in poly (lactic-coglycolic acid) nanoparticles (PLGA-Crt NPs) to investigate its resistance to cisplatin-resistant human ovarian carcinoma cell line (A2780-RCIS). The results showed that PLGA-Crt NPs (25, 50, 100, 200 μmol) could reduce the gene expression and efflux function of multidrug resistance protein 2 (MRP2) transporters in cisplatin-resistant A2780-RCIS to inhibit cell resistance (Neyshaburinezhad et al, 2019).…”

Section: Ovarian Cancermentioning

confidence: 99%

Crocetin: A Systematic Review

GUO

et al. 2022

Front. Pharmacol.

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Crocetin is an aglycone of crocin naturally occurring in saffron and produced in biological systems by hydrolysis of crocin as a bioactive metabolite. It is known to exist in several medicinal plants, the desiccative ripe fruit of the cape jasmine belonging to the Rubiaceae family, and stigmas of the saffron plant of the Iridaceae family. According to modern pharmacological investigations, crocetin possesses cardioprotective, hepatoprotective, neuroprotective, antidepressant, antiviral, anticancer, atherosclerotic, antidiabetic, and memory-enhancing properties. Although poor bioavailability hinders therapeutic applications, derivatization and formulation preparation technologies have broadened the application prospects for crocetin. To promote the research and development of crocetin, we summarized the distribution, preparation and production, total synthesis and derivatization technology, pharmacological activity, pharmacokinetics, drug safety, drug formulations, and preparation of crocetin.

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Encapsulation of crocetin into poly (lactic-co-glycolic acid) nanoparticles overcomes drug resistance in human ovarian cisplatin-resistant carcinoma cell line (A2780-RCIS)

Cited by 12 publications

References 33 publications

Crocetin Extracted from Saffron Shows Antitumor Effects in Models of Human Glioblastoma

Crocetin Extracted from Saffron Shows Antitumor Effects in Models of Human Glioblastoma

The nanocomposites designs of phytomolecules from medicinal and aromatic plants: promising anticancer-antiviral applications

Crocetin: A Systematic Review

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