Encapsulation of entomopathogenic fungal conidia: evaluation of stability and control potential of Rhipicephalus microplus

Meirelles, Laura Nóbrega; Mesquita, Emily; Corrêa, Thaís Almeida; Bitencourt, Ricardo de Oliveira Barbosa; Oliveira, Jhones Luiz de; Fraceto, Leonardo Fernandes; Camargo, Mariana Guedes; Bittencourt, Vânia Rita Elias Pinheiro

doi:10.1016/j.ttbdis.2023.102184

Cited by 9 publications

(6 citation statements)

References 41 publications

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“…A diferencia de lo que se observó con Th003, el tiempo de lectura evidenció un efecto significativo sobre el número de conidios germinados a las 18 y a las 24 h, en el medio AA suplementado con benomil ajustado a 0,003 % y 0,0005 %, respectivamente. Este resultado evidenció el efecto negativo que ejerce la adición de benomil en ambas concentraciones en el medio AA y valida la influencia que tienen las sustancias sincronizadoras sobre la viabilidad de los conidios (Francisco et al, 2006;Meirelles et al 2023).…”

Section: Resultados Y Discusiónunclassified

Parámetros adecuados para la evaluación de germinación de cepas élite de hongos biocontroladores

Uribe Gutiérrez,

Santos-Diaz

2024

SIEMBRA

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La comercialización de plaguicidas microbianos ha aumentado de manera exponencial en los últimos años. Casi el 70 % de los productos que se comercializan utilizan como principio activo conidios de hongos biocontroladores y uno de los criterios más utilizados para evaluar la calidad de estos productos es la prueba de germinación de conidios. Específicamente, se utiliza para la selección y la evaluación de la viabilidad y vigor de cepas élite con potencial biológico. Algunos de los factores que influyen en la germinación de los conidios incluye la selección del medio del cultivo, la temperatura, el tiempo de incubación y la adición de sustancias sincronizadoras de germinación. La optimización para cada cepa élite permite estimar el comportamiento del microorganismo, lo que garantizará su actividad biológica y eficacia, al ser un recurso biológico con fines comerciales. El objetivo de este trabajo fue seleccionar las condiciones óptimas (medios de cultivo, tiempo de incubación y concentración de sustancia sincronizadora) para la germinación de tres cepas élite Trichoderma koningiopsis (Th003), Lecanicillium lecanii (Vl026) y Metarhizium robertsii (Mt004), las cuales son principios activos de bioplaguicidas en proceso de comercialización. Como resultado se seleccionó el medio de cultivo de agar agua con benomil al 0,0003 % con un tiempo de lectura de 18 h para Th003 y de 24 h para Vl026. Para la cepa Mt004, el medio de cultivo que favoreció la germinación sincrónica de los conidios fue agar PDA suplementado con benomil al 0,0003 % con un tiempo de lectura de 18 h. Estas condiciones permitirán a través del tiempo realizar una correcta evaluación de la germinación de los agentes de control biológico y garantizar su viabilidad y vigor como principio activo de bioplaguicidas.

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Section: Resultados Y Discusiónunclassified

Parámetros adecuados para la evaluación de germinación de cepas élite de hongos biocontroladores

Uribe Gutiérrez,

Santos-Diaz

2024

SIEMBRA

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“…Unfortunately, industry places little emphasis on shelf life and technologies that improve insect pathogenicity and delivery. There is a perceived lack of investment and interest in formulation research to boost efficacy, although the relevance of this improvement has already been reported (Vemmer and Patel, 2013;Iwanicki et al, 2021;Marciano et al, 2021;Meirelles et al, 2023). Nonetheless, this is not peculiar to Brazil, especially due to the time required to approve a novel formulation.…”

Section: Commercial Products In Brazilmentioning

confidence: 99%

Utilization of Metarhizium as an insect biocontrol agent and a plant bioinoculant with special reference to Brazil

Mesquita,

Hu,

Lima

et al. 2023

Front. Fungal Biol.

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Brazil has a long history of using biological control and has the largest program in sugarcane agriculture to which a biocontrol program has been applied. This achievement is at least partly due to the utilization of the entomopathogenic fungus Metarhizium. This well-known fungal genus exhibits pathogenicity against a broad range of arthropod hosts and has been used globally as a biocontrol agent. This fungus is also a root symbiont, and in this capacity, it is a plant growth promoter. However, this feature (i.e., as a plant symbiont) has yet to be fully explored and implemented in Brazil, although the number of reports demonstrating Metarhizium’s utility as a plant bioinoculant is increasing. The Brazilian bioproduct industry targets agricultural pests, and is limited to two Metarhizium species represented by four fungal isolates as active ingredients. Entomopathogenic fungi have also been successful in controlling arthropods of public health concern, as shown in their control of mosquitoes, which are vectors of diseases. The isolation of new indigenous Metarhizium isolates from a variety of substrates such as soil, insects, and plants shows the wide genetic diversity within this fungal genus. In this review, we emphasize the significance of Metarhizium spp. for the biological control of insects in Brazil. We also suggest that the experience and success of biological control with fungi in Brazil is an important resource for developing integrated pest management and sustainable strategies for pest control worldwide. Moreover, the future implementation prospects of species of Metarhizium being used as bioinoculants and possible new advances in the utility of this fungus are discussed.

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“…These issues raise the need to develop alternative control methods, including the selection of parasite-resistant breeds [12,13]; biological control using entomopathogenic fungi (Metarhizium spp., Beauveria spp.) [14,15]; entomopathogenic nematodes (Heterorhabditidae and Steinernematidae) [16,17]; regulator ants (Solenopsis germinata, S. saevissima, Camponotus rengira, and Ectatomma quadridens) [18,19]; pesticides [20,21]; and immunological control through the application of anti-tick vaccines [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Rabbits as Animal Models for Anti-Tick Vaccine Development: A Global Scenario

Rodríguez-Durán,

Ullah,

Parizi

et al. 2023

Pathogens

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Studies evaluating candidate tick-derived proteins as anti-tick vaccines in natural hosts have been limited due to high costs. To overcome this problem, animal models are used in immunization tests. The aim of this article was to review the use of rabbits as an experimental model for the evaluation of tick-derived proteins as vaccines. A total of 57 tick proteins were tested for their immunogenic potential using rabbits as models for vaccination. The most commonly used rabbit breeds were New Zealand (73.8%), Japanese white (19%), Californians (4.8%) and Flemish lop-eared (2.4%) rabbits. Anti-tick vaccines efficacy resulted in up to 99.9%. Haemaphysalis longicornis (17.9%) and Ornithodoros moubata (12.8%) were the most common tick models in vaccination trials. Experiments with rabbits have revealed that some proteins (CoAQP, OeAQP, OeAQP1, Bm86, GST-Hl, 64TRP, serpins and voraxin) can induce immune responses against various tick species. In addition, in some cases it was possible to determine that the vaccine efficacy in rabbits was similar to that of experiments performed on natural hosts (e.g., Bm86, IrFER2, RmFER2, serpins and serine protease inhibitor). In conclusion, results showed that prior to performing anti-tick vaccination trials using natural hosts, rabbits can be used as suitable experimental models for these studies.

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Encapsulation of entomopathogenic fungal conidia: evaluation of stability and control potential of Rhipicephalus microplus

Cited by 9 publications

References 41 publications

Parámetros adecuados para la evaluación de germinación de cepas élite de hongos biocontroladores

Parámetros adecuados para la evaluación de germinación de cepas élite de hongos biocontroladores

Utilization of Metarhizium as an insect biocontrol agent and a plant bioinoculant with special reference to Brazil

Rabbits as Animal Models for Anti-Tick Vaccine Development: A Global Scenario

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