Encapsulation of functional cells by sol–gel silica: actual progress and perspectives for cell therapy

Carturan, G.; Toso, Roberto Dal; Boninsegna, Sara; Monte, Renzo Dal

doi:10.1039/b401450b

Cited by 190 publications

(168 citation statements)

References 87 publications

(127 reference statements)

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“…While the encapsulation of cells was one of the first studies in the field, 26 after only modest interest for a number of years, 27 the topic of cell encapsulation is rapidly gaining renewed interest in part because of new synthetic routes. 28,29 In this section, we highlight some of the features that have led to the emergence of sol-gel encapsulation of biomolecules. We conclude the section with a discussion of future directions.…”

Section: Encapsulation Of Biological Molecules In Sol-gel Matricesmentioning

confidence: 99%

“…Recent reviews have emphasized the increased interest in the sol-gel encapsulation of whole cells. 25,28 As indicated previously, the development of new synthetic approaches has overcome many of the alcohol and pH limitations experienced in earlier work, enabling the field to make progress in a number of areas. With the ability of cells to be used in a variety of areas, including biosynthesis, sensing, and as scaffolds for cell and tissue growth, it is evident that the cell encapsulation area will be an active one in the coming years.…”

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confidence: 99%

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Molecules in Glass: Probes, Ordered Assemblies, and Functional Materials

Dunn¹,

Zink²

2007

ChemInform

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Research on the properties and applications of molecules doped into sol-gel-derived silica matrices has expanded rapidly. This Account begins with a brief review of the use of the dopant molecules as probes of the changes that occur as the system evolves from the initial sol to the final xerogel during the formation of monoliths, thin films, and mesostructured films. Methods of deliberately placing desired molecules in specific regions of the mesostructure are discussed, and an application, energy transfer, is presented. Finally, encapsulation of biological molecules is examined, and two important aspects, stabilization of the biomolecules and applications as biosensors, are described.

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Section: Encapsulation Of Biological Molecules In Sol-gel Matricesmentioning

confidence: 99%

mentioning

confidence: 99%

Molecules in Glass: Probes, Ordered Assemblies, and Functional Materials

Dunn¹,

Zink²

2007

ChemInform

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“…317,318 However, many other applications require biohybrids with encapsulated living cells and microorganisms, for use as bioreactors profiting from their metabolic activity for production of beneficial compounds, [319][320][321] as well as in other interesting applications within the biomedical field. [322][323][324] Selected examples of the possible applications of these materials are summarized in Table 7.…”

Section: Silica and Silicate Biohybrids Incorporating Whole Cells Andmentioning

confidence: 99%

Hybrid and biohybrid silicate based materials: molecular vs. block-assembling bottom–up processes

2011

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“…Moisture in the atmosphere and on the cell surface allows for the reaction of the alkoxysilanes and deposition of a sol-gel shell. When implanted, the direct sol-gel silica tissue interface did not demonstrate signs of inflammatory cell aggregation or an immune reaction, providing evidence for the biocompatibility of the material used in tissue encapsulation applications (5).…”

mentioning

confidence: 92%

“…These implants were able to supply enough insulin to prevent urinary excretion of glucose in NOD mice for almost 11 wk (35); however, bulk materials can create issues by creating diffusion barriers, increasing hypoxia, increasing transplant volume, and inserting delays in the glucose-insulin control system. Carturan and colleagues achieved thin silica encapsulation of islets by using the Biosil process (5,33). This method involves exposing cells to a gas stream comprised of N 2 2 ].…”

mentioning

confidence: 99%

Mouse and human islets survive and function after coating by biosilicification

Jaroch

Madangopal

et al. 2013

American Journal of Physiology-Endocrinology and Metabolism

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Inorganic materials have properties that can be advantageous in bioencapsulation for cell transplantation. Our aim was to engineer a hybrid inorganic/soft tissue construct by inducing pancreatic islets to grow an inorganic shell. We created pancreatic islets surrounded by porous silica, which has potential application in the immunoprotection of islets in transplantation therapies for type 1 diabetes. The new method takes advantage of the islet capsule surface as a template for silica formation. Mouse and human islets were exposed to medium containing saturating silicic acid levels for 9 -15 min. The resulting tissue constructs were then cultured for up to 4 wk under normal conditions. Scanning electron microscopy and energy dispersive Xray spectroscopy was used to monitor the morphology and elemental composition of the material at the islet surface. A cytokine assay was used to assess biocompatibility with macrophages. Islet survival and function were assessed by confocal microscopy, glucose-stimulated insulin release assays, oxygen flux at the islet surface, expression of key genes by RT-PCR, and syngeneic transplant into diabetic mice. islet; encapsulation; silica; coating; tissue engineering TO CREATE 3D TISSUE CONSTRUCTS for transplantation therapies, cells are typically encapsulated within organic polymers, either synthetic or natural, such as collagen or alginate. For applications requiring protection from the immune system, however, inorganic or hybrid (inorganic/organic) materials may offer a different set of useful functions and properties to be used as alternatives to or in conjunction with the organic polymers. Porous silica is an attractive material for these applications because of its mesoporosity, potential to create hierarchical structures, optical transparency, capacity for biofunctionalization, performance in precision size exclusion, and bioactivity.Our work focuses on primary pancreatic islets because of their importance in therapies for type 1 diabetes. The Edmonton protocol of islet transplantation in humans (40) has demonstrated remarkable short-term success, with 80% of individuals achieving insulin independence at 1 yr posttransplant; however, this rate decreases to only about 10 -15% at 5 yr (3). Multiple mechanisms contribute to the progressive loss of graft function but likely include an immediate blood-mediated response (IBMR) (1, 37), poor revascularization and hypoxia (9, 16), and ongoing auto-and alloimmune responses (38). Protection of islets or stem cell-derived pseudo-islets by protective membranes could significantly improve transplant outcomes for patients.The sol-gel synthesis method of forming solid porous silica under biologically friendly temperatures and pH has been used to encapsulate first biomolecules and later living cells since the early 1990s. Early attempts to use silica as a microencapsulation matrix for islets employed bulk sol-gel techniques, resulting in islets encased in thick silica slabs or spheres (34, 35). These implants were able to supply enough insulin...

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Encapsulation of functional cells by sol–gel silica: actual progress and perspectives for cell therapy

Cited by 190 publications

References 87 publications

Molecules in Glass: Probes, Ordered Assemblies, and Functional Materials

Molecules in Glass: Probes, Ordered Assemblies, and Functional Materials

Hybrid and biohybrid silicate based materials: molecular vs. block-assembling bottom–up processes

Mouse and human islets survive and function after coating by biosilicification

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