Encapsulation of human elastic cartilage-derived chondrocytes in nanostructured fibrin-agarose hydrogels

García-Martínez, Laura; Campos, Fernando; Godoy-Guzmán, Carlos; Sánchez-Quevedo, María del Carmen; Garzón, Ingrid; Alaminos, Miguel; Campos, Antonìo; Carriel, Víctor

doi:10.1007/s00418-016-1485-9

Cited by 55 publications

(54 citation statements)

References 57 publications

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“…Immunostaining was contrasted with Harris hematoxylin. Images were obtained using a Nikon Eclipse 90i light microscope (Nikon Corp., Tokyo, Japan) and the immunohistochemical signal intensity was semiquantitatively assessed as strong (+++), mild (++), slight (+) or negative (−) as previously described (Garcia-Martinez et al 2017). Samples immunostained with anti-Melan A antibodies were used to quantify the number of melanocyte cells per 100 µm of length of the epidermis in each sample, CD1A was used to quantify Langerhans cells and CK20 was used as a marker of Merkel cells.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the human ridged and non-ridged skin: a comprehensive histological, histochemical and immunohistochemical analysis

Vela-Romera

Carriel

Martín-Piedra

et al. 2018

Histochem Cell Biol

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The structure of the human skin is directly dependent on its location and the mechanical forces to which it is subjected. In the present work, we have performed a comprehensive analysis of the human ridged and non-ridged skin to identify the differences and similarities between both skin types. For this purpose, human skin samples were obtained from dorsal hand skin (DHS), palmar hand skin (PHS), dorsal foot skin (DFS) and plantar foot skin (PFS) from the same cadaveric donors. Histological, histochemical and semiquantitative and quantitative immunohistochemical analyses were carried out to evaluate the epidermis, dermis and basement membrane. Results show that the epithelial layer of ridged skin had larger cell number and size than non-ridged skin for most strata. Melanocytes and Langerhans cells were more abundant in non-ridged skin, whereas Merkel cells were preferentially found in ridged skin. The expression pattern of CK5/6 was slightly differed between non-ridged and ridged skin. Involucrin expression was slightly more intense in non-ridged skin than in ridged skin. Collagen was more abundant in foot skin dermis than in hand skin, and in ridged skin as compared to non-ridged skin. Elastic fibers were more abundant in DHS. Biglycan was more abundant in foot skin than in hand skin. No differences were found for blood and lymphatic vessels. The basement membrane laminin was preferentially found in foot skin. These results revealed important differences at the epithelial, dermal and basement membrane levels that could contribute to a better knowledge of the human skin histology.

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Section: Methodsmentioning

confidence: 99%

Characterization of the human ridged and non-ridged skin: a comprehensive histological, histochemical and immunohistochemical analysis

Vela-Romera

Carriel

Martín-Piedra

et al. 2018

Histochem Cell Biol

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“…2017c; Garcia‐Martinez et al. ). Briefly, all steps were performed in a humid chamber to avoid dehydration of the sections.…”

Section: Methodsmentioning

confidence: 99%

Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis

Godoy-Guzmán

Nuñez²,

Orihuela

et al. 2018

Journal of Anatomy

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The uterine tube (UT) is an important and complex organ of the women's reproductive system. In general, the anatomy and basic histology of this organ are well-known. However, the composition and function of the extracellular matrix (ECM) of the UT is still poorly understood. The ECM is a complex supramolecular material produced by cells which is commonly restricted to the basement membrane and interstitial spaces. ECM molecules play not only a structural role, they are also important for cell growth, survival and differentiation in all tissues. In this context, the aim of this study was to evaluate the deposition and distribution of type I and III collagens and proteoglycans (decorin, biglycan, fibromodulin and versican) in human UT during the follicular and luteal phases by using histochemical and immunohistochemical techniques. Our results showed a broad synthesis of collagens (I and III) in the stroma of the UT. The analysis by regions showed, in the mucosa, a specific distribution of versican and fibromodulin in the epithelial surface, whereas decorin and fibromodulin were observed in the lamina propria. Versican and decorin were found in the stroma of the muscular layer, whereas all studied proteoglycans were identified in the serosa. Curiously, biglycan was restricted to the wall of the blood vessels of the serosa and muscular layers. Furthermore, there was an immunoreaction for collagens, decorin, versican and fibromodulin in the UT peripheral nerves. The differential distribution of these ECM molecules in the different layers of the UT could be related to specific structural and/or biomechanical functions needed for the oviductal transport, successful fertilization and early embryogenesis. However, further molecular studies under physiological and pathological conditions are still needed to elucidate the specific role of each molecule in the human UT.

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“…Standard protocols of immunohistochemistry (IHC) (Garcia‐Martinez, Campos, & Godoy‐Guzman, ; Godoy‐Guzman, San Martin, & Pereda, ) and confocal immunofluorescence (Itoh, Itoh, Tassoni, & Ren, ; Sun et al, ) were performed as described earlier with minor modifications. For both methods, tissue sections of 5 μm thick were deparaffinized in xylene and rehydrated through graded sets of ethyl alcohol.…”

Section: Methodsmentioning

confidence: 99%

Expression of glial cells molecules in the optic nerve of adult dromedary camel (Camelus dromedarius): A histological and immunohistochemical analysis

Metwally

Farouk

Hossain

et al. 2018

Anat Histol Embryol

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The optic nerve (ON) is an important organ in the visual system of animals, which transfers electrical impulses towards the brain from the retina. High enrichment of glial cells in ON is known to support neuron and regulate retinal homoeostasis. However, research on immunohistochemical of glial cells proteins in the camel is scanty in available literature. Hence, the current work is an attempt to investigate the histomorphology of camel ON with regard to the expression patterns of glial fibrillary acidic protein (GFAP), myelin basic protein (MBP) and Iba1 for the three glial subtypes, namely astrocytes, oligodendrocytes and microglia, respectively. Optic nerves from fourteen dromedary camels were dissected and preserved in 10% formalin. Then, the paraffin‐embedding sections were subjected for histochemical and immunohistochemical analysis. Our results demonstrated that ON axons aggregate into fascicles that surrounded by light and densely stained glial cells. Then, we examined the myelin sheath using Heidenhain's and Mallory's phosphotungstic acid staining. Immunoassay results revealed that GFAP is enriched in the ON and distributed evenly, whereas MBP and Iba1 were present at scanty levels. Further analysis of mRNA level of GFAP, MBP and Iba1 in the ON confirmed an elevation of GFAP expression compared to MBP and Iba1. We further found partial co‐localization of different types of glial cells that reflect their coordinated function in the ON. Although our data provide the first evidence for differential expression pattern of glial proteins, further molecular studies still required to reveal the specific function of these molecules in the camel ON.

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Encapsulation of human elastic cartilage-derived chondrocytes in nanostructured fibrin-agarose hydrogels

Cited by 55 publications

References 57 publications

Characterization of the human ridged and non-ridged skin: a comprehensive histological, histochemical and immunohistochemical analysis

Characterization of the human ridged and non-ridged skin: a comprehensive histological, histochemical and immunohistochemical analysis

Distribution of extracellular matrix molecules in human uterine tubes during the menstrual cycle: a histological and immunohistochemical analysis

Expression of glial cells molecules in the optic nerve of adult dromedary camel (Camelus dromedarius): A histological and immunohistochemical analysis

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