Encapsulation of mammalian cell with chitosan‐CMC capsule

Yoshioka, Toshimitsu; Hirano, Ryogo; Shioya, Toshiaki; Kako, Masatoshi

doi:10.1002/bit.260350110

Cited by 107 publications

(40 citation statements)

References 37 publications

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“…The peaks at 850 and 1157 cm À1 correspond to the saccharide structure. 34 When two or more substances are mixed, physical blends vs. chemical interactions are reflected by changes in the characteristic spectral peaks. 35 In the typical spectrum of the 1:1 starch-chitosan composite film, the characteristic peak of starch at 1659 cm À1 and the amide peaks of chitosan at 1633 and 1580 cm À1 shifted to a higher frequency, indicating that there was interaction between the NH 3 þ and hydroxyl groups of starch, which improved the compatibility between them, while the amide III peak at 1314 cm…”

Section: Ftir Spectroscopymentioning

confidence: 99%

Microstructural imaging and characterization of the mechanical, chemical, thermal, and swelling properties of starch–chitosan blend films

2006

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Chitosan has wide range of applications as a biomaterial, but barriers still exist to its broader use due to its physical and chemical limitations. The present study evaluated the properties of the polymeric blend films obtained from chitosan and potato starch by the casting/solvent evaporation method. The swelling properties of the different films studied as a function of pH showed that the sorption ability of the blend films increased with the increasing content of starch. Fourier transform infrared (FTIR) analyses confirmed that interactions were present between the hydroxyl groups of starch and the amino groups of chitosan in the blend films while the x-ray diffraction (XRD) studies revealed the films to exhibit an amorphous character. Thermogravimetric analyses showed that in the blend films, the thermal stability increased with the increasing starch content and the stability of starch and chitosan powders reduced when they were converted to film. The differential scanning calorimetry (DSC) studies revealed an endotherm corresponding to water evaporation around 100 degrees C in all the films and an exotherm, corresponding to the decomposition in the chitosan and blend films. Scanning electron microscopy (SEM) observations indicated that the blend films were less homogenous and atomic force microscopy (AFM) studies revealed the chitosan films to be smooth and homogenous, while the starch films revealed characteristic granular pattern. The blend films exhibited an intermediate character with a slight microphase separation. The starch-chitosan blend films exhibited a higher flexibility and incorporation of potato starch into chitosan films improved the percentage elongation.

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Section: Ftir Spectroscopymentioning

confidence: 99%

Microstructural imaging and characterization of the mechanical, chemical, thermal, and swelling properties of starch–chitosan blend films

2006

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“…Wang developed a simple and ef®cient method to encapsulate cells in only one step [4]. The monoclonal antibodies produced by the encapsulated cells remained in the chitosan-CMC capsule [5] while the products of the cells entrapped in the bead leaked into broth solution. In the alginate-polylysine microcapsules, intracapsular monoclonal antibody concentration was 5300 lg/ml [6].…”

Section: List Of Symbolsmentioning

confidence: 99%

The effect of oxygen transfer on the activity of encapsulated whole cell β-galactosidase

Park

Jeong

Chang

1997

Bioprocess Engineering

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The effect of oxygen transfer on the production of immobilized whole cell b-galactosidase has been evaluated. The encapsulated whole cell b-galactosidase was prepared by combining cell encapsulation and culture into one-step. Escherichia coli was encapsulated and cultured in the growth and production media to accumulate b-galactosidase in itself. Sun¯ower seed oil was coimmobilized to increase the oxygen transfer rate through the capsule membrane. The oxygen transfer rate increased 63 percent and the activity of b-galactosidase increased by 10 percent. The activity of encapsulated b-galactosidase obtained in the concentric air lift reactor was 86 percent higher than that in the shaking incubator. In the concentric air lift reactor, the accumulation of encapsulated whole cell b-galactosidase was primarily dependent on the capsule velocity. While the accumulation of speci®c b-galactosidase in the capsule increased with volumetric oxygen transfer coef®cient, the cell biomass accumulated in the capsule decreased.

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“…The membrane of the capsule should be designed such that the required nutrients and cell products can easily pass through it (Groboillot et al, 1994;Jen et al, 1996). Encapsulation can be carried out by using either natural or synthetic polymers such as calcium alginate (Cheong et al, 1993), carrageenan-oligochitosan (Bartkowiak and Hunkeler, 2001), chitosan-CMC (Yoshioka et al, 1990), alginate-poly-L-lysine (Lim and Sun 1980), alginateoligochitosan (Bartkowiak and Hunkeler, 1999), alginateaminopropylsilicate (Sakai et al, 2002), agarose (Nigam et al, 1988), and polyamide (Green et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Ethanol production from glucose and dilute‐acid hydrolyzates by encapsulated S. cerevisiae

Talebnia

Niklasson

Taherzadeh

2005

Biotech & Bioengineering

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The performance of encapsulated Saccharomyces cerevisiae CBS 8066 in anaerobic cultivation of glucose, in the presence and absence of furfural as well as in dilute-acid hydrolyzates, was investigated. The cultivation of encapsulated cells in 10 sequential batches in synthetic media resulted in linear increase of biomass up to 106 g/L of capsule volume, while the ethanol productivity remained constant at 5.15 (+/-0.17) g/L x h (for batches 6-10). The cells had average ethanol and glycerol yields of 0.464 and 0.056 g/g in these 10 batches. Addition of 5 g/L furfural decreased the ethanol productivity to a value of 1.31 (+/-0.10) g/L x h with the encapsulated cells, but it was stable in this range for five consecutive batches. On the other hand, the furfural decreased the ethanol yield to 0.41-0.42 g/g and increased the yield of acetic acid drastically up to 0.068 g/g. No significant lag phase was observed in any of these experiments. The encapsulated cells were also used to cultivate two different types of dilute-acid hydrolyzates. While the free cells were not able to ferment the hydrolyzates within at least 24 h, the encapsulated yeast successfully converted glucose and mannose in both of the hydrolyzates in less than 10 h with no significant lag phase. However, since the hydrolyzates were too toxic, the encapsulated cells lost their activity gradually in sequential batches.

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Encapsulation of mammalian cell with chitosan‐CMC capsule

Cited by 107 publications

References 37 publications

Microstructural imaging and characterization of the mechanical, chemical, thermal, and swelling properties of starch–chitosan blend films

Microstructural imaging and characterization of the mechanical, chemical, thermal, and swelling properties of starch–chitosan blend films

The effect of oxygen transfer on the activity of encapsulated whole cell β-galactosidase

Ethanol production from glucose and dilute‐acid hydrolyzates by encapsulated S. cerevisiae

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