Encapsulation of micropropagated buds of six woody species

Piccioni, Emanuele; Standardi, A.

doi:10.1007/bf00029990

Cited by 79 publications

(42 citation statements)

References 6 publications

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“…The use of ABA as a growth retardant might contribute to a new understanding of the optimization of storage conditions in certain plant species, having highly active meristematic tissues for germination within hollow gel beads soon after encapsulation. There are several reports on synthetic seed production of fruit plants based on in vitro conservation and storage at 4 °C, including pomegranate (Naik and Chand, 2006), mulberry (Kavyashree et al, 2006), raspberry (Piccioni and Standardi, 1995), and black nightshade (Verma et al, 2010). Plant genotype, explant type, concentration of mineral contents, and/or sucrose in medium storage conditions and growth regulators are crucial for testing the viability and conservation of synthetic seeds (Benelli et al, 2013;Sharma et al, 2013;Berjak et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Clonal propagation and synthetic seed production from nodal segments ofCape gooseberry (Physalis peruviana L.), a tropical fruit plant

Yücesan

Mohammed²,

Arslan³

et al. 2015

Turk J Agric For

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IntroductionPhysalis peruviana L. is a species from the family Solanaceae and genus Physalis, commonly known as Cape gooseberry (also known as physalis, golden berry, etc.). It is an herbaceous, semishrub, perennial plant, native to tropical Peru and other warm temperate and subtropical regions throughout the world (Ramirez et al., 2013). Cape gooseberry resembles tomato in flavor and appearance, though the sour and sweet taste is richer in this tropical fruit. This plant has increasing popularity due to its nutritional and medicinal values. Cape gooseberry is a source of health-related compounds found in the fruits and other parts of the plant, including leaves and stems. It has been widely used for treating diabetes, hepatitis, ulcers, and several other diseases (Mayorga et al., 2002;Arun and Asha, 2007). It also contains high levels of vitamins A, C, and B-complex, as well as compounds with antiinflammatory and antioxidant properties (Strik, 2007). Wu et al. (2009) reported that supercritical carbon dioxide extracts of P. peruviana leaves induced cell cycle arrest and apoptosis in human lung cancer cells. Similarly, Çakir et al. (2014) reported that phenolic content of ethanolic leaf extracts (100 µg mL -1 ) possessed high cytotoxic effects on HeLa cells (an immortal cell line).The use of tissue culture methods for the selection of the best cultivars for agricultural practices and clonal propagation might be conducive for micropropagation, especially when taking into account high consumption due to value-added natural compounds, limited plant production, and requirements of manpower, transport, and storage (Rodrigues et al., 2013a). Though P. peruviana was reported as recalcitrant for shoot organogenesis through adventitious regeneration from leaf explants (Torres, 1991), there are a limited number of in vitro regeneration studies concerning the micropropagation of P. peruviana (Rodrigues et al., 2013b), not containing information about the synergistic effects of plant growth regulator (PGR) combinations on growth parameters. Several studies on in vitro propagation of other Physalis species, including P. minima, P. ixocarpa, and P. pruinosa,

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Section: Discussionmentioning

confidence: 99%

Clonal propagation and synthetic seed production from nodal segments ofCape gooseberry (Physalis peruviana L.), a tropical fruit plant

Yücesan

Mohammed²,

Arslan³

et al. 2015

Turk J Agric For

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“…In some species, such as banana, cardamom, mulberry and raspberry, encapsulated microcuttings demonstrated high adventitious rooting capacity after sowing [14,24,25], while other species did not respond [23,26]. There are many factors affect the conversion of synseeds after cold storage period.…”

Section: Introductionmentioning

confidence: 99%

Conversion of Banana Synseed Influenced by the Bead Type and Seed Coat

Hassanein¹,

Ibraheim²,

Galal³

et al. 2011

AJPS

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“…In addition, Mathur et al (1989) reported that the use of this technology economized upon the medium, space and time requirements. Successful cases of synthetic seeds production and plantlet regeneration have been reported for cereals, vegetables, fruits, ornamentals, aromatic grass and conifers (Fowke et al, 1994;Piccioni and Standardi, 1995;Castillo et al, 1998;Ganapathi et al, 2001;Brischia et al, 2002;Hao and Deng, 2003). However, in most cases somatic embryos were used in the encapsulation process.…”

Section: Introductionmentioning

confidence: 99%

“…However, in most cases somatic embryos were used in the encapsulation process. Some authors (Mathur et al, 1989;Ganapathi et al, 1992;Sharma et al, 1994;Piccioni and Standardi, 1995;Pattnaik and Chand, 2000) described the encapsulation of vegetative propagules such as axillary buds or shoot tips, which could be used for mass clonal propagation as well as in longterm conservation of germplasm.…”

Section: Introductionmentioning

confidence: 99%

Propagation through alginate encapsulation of axillary buds of Cannabis sativa L. — an important medicinal plant

Lata

Chandra

Khan

et al. 2009

Physiol Mol Biol Plants

104

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Cannabis sativa L. (Cannabaceae) is an important medicinal plant well known for its pharmacologic and therapeutic potency. Because of allogamous nature of this species, it is difficult to maintain its potency and efficacy if grown from the seeds. Therefore, chemical profile-based screening, selection of high yielding elite clones and their propagation using biotechnological tools is the most suitable way to maintain their genetic lines. In this regard, we report a simple and efficient method for the in vitro propagation of a screened and selected high yielding drug type variety of Cannabis sativa, MX-1 using synthetic seed technology. Axillary buds of Cannabis sativa isolated from aseptic multiple shoot cultures were successfully encapsulated in calcium alginate beads. The best gel complexation was achieved using 5 % sodium alginate with 50 mM CaCl 2 .2H 2 O. Regrowth and conversion after encapsulation was evaluated both under in vitro and in vivo conditions on different planting substrates. The addition of antimicrobial substance -Plant Preservative Mixture (PPM) had a positive effect on overall plantlet development. Encapsulated explants exhibited the best regrowth and conversion frequency on Murashige and Skoog medium supplemented with thidiazuron (TDZ 0.5 μM) and PPM (0.075 %) under in vitro conditions. Under in vivo conditions, 100 % conversion of encapsulated explants was obtained on 1:1 potting mix-fertilome with coco natural growth medium, moistened with full strength MS medium without TDZ, supplemented with 3 % sucrose and 0.5 % PPM. Plantlets regenerated from the encapsulated explants were hardened off and successfully transferred to the soil. These plants are selected to be used in mass cultivation for the production of biomass as a starting material for the isolation of THC as a bulk active pharmaceutical.

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Encapsulation of micropropagated buds of six woody species

Cited by 79 publications

References 6 publications

Clonal propagation and synthetic seed production from nodal segments ofCape gooseberry (Physalis peruviana L.), a tropical fruit plant

Clonal propagation and synthetic seed production from nodal segments ofCape gooseberry (Physalis peruviana L.), a tropical fruit plant

Conversion of Banana Synseed Influenced by the Bead Type and Seed Coat

Propagation through alginate encapsulation of axillary buds of Cannabis sativa L. — an important medicinal plant

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