Encapsulation of protamine sulphate compacted DNA in polylactide and polylactide–co-glycolide microparticles

Dunne, Mairéad; Bibby, David C.; Jones, Joanna C.; Cudmore, Sally

doi:10.1016/s0168-3659(03)00304-3

Cited by 36 publications

(28 citation statements)

References 13 publications

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“…To optimize pDNA-loaded NPs as gene vector, using protamines or arginine during formulation in the future may be benefit for a higher encapsulation efficiency and a greater protection. 77,78 Taken together, our findings showed that application of HIF-1a-directed shRNA significantly reduced the extent of neovascularization in the murine laser photocoagulation model of CNV. PLGA NPs 'protect' the carried genetic material and obtain a sustained release rate, in addition to offering an increased facility of tissue penetration and intracellular uptake.…”

Section: Pdna-loaded Nanoparticles Inhibit Cnv C Zhang Et Alsupporting

confidence: 61%

Inhibitory efficacy of hypoxia-inducible factor 1α short hairpin RNA plasmid DNA-loaded poly (D, L-lactide-co-glycolide) nanoparticles on choroidal neovascularization in a laser-induced rat model

Zhang

et al. 2009

Gene Ther

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The aim of this study was to evaluate the possibility of poly (D, L-lactide-co-glycolide) nanoparticle (NPs) as a gene vector for functional plasmid DNA (pDNA) and to investigate its inhibitory efficacy on experimental choroidal neovascularization (CNV). We developed intravitreal administered, hypoxia-inducible factor 1a (HIF-1a) short hairpin RNA and green fluorescent protein (GFP) co-expressed pDNA-loaded NPs (pshHIF-1a NPs). CNV was induced by laser photocoagulation in 112 rats. The rats were then randomly assigned to be injected intravitreally with phosphate-buffered saline (PBS), blank NPs, naked pDNA, control pDNA NPs and pshHIF-1a NPs, respectively, and non-injection group was set as the control. Immunofluorescence staining, fluorescein fundus angiography and histologic analysis were performed to evaluate the inhibitory efficacy on CNV. The results showed that the expression of GFP preferentially localized in the retinal pigment epithelium cell layer and lasted for 4 weeks. The fluorescein leakage areas of CNV were significantly larger in the PBS, blank NPs, control pDNA NPs, non-injection group and naked pDNA group than in pshHIF-1a NPs group (Po0.01). The mean thickness of the CNV lesions in the intravitreally pshHIF1a NPs-treated group was significantly smaller than other groups (Po0.01). No signs of functional or ultrastructural destruction in retina were detected. Therefore, pshHIF-1a NPs may act as a novel therapeutic option to transfer specific pDNA and inhibit the formation of experimental CNV.

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Section: Pdna-loaded Nanoparticles Inhibit Cnv C Zhang Et Alsupporting

confidence: 61%

Inhibitory efficacy of hypoxia-inducible factor 1α short hairpin RNA plasmid DNA-loaded poly (D, L-lactide-co-glycolide) nanoparticles on choroidal neovascularization in a laser-induced rat model

Zhang

et al. 2009

Gene Ther

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“…A novel nanoparticle formulation was prepared in a simple two-steps process using cholesterol, DOTAP, protamine sulfate (PS), and high mobility group protein (HMG-1). Although, cholesterol, DOTAP, and protamine sulfate have been used either previously16, individually or in combination1017 for transfecting various cells, this is the first time we are reporting a unique composition and method for transfecting P. falciparum infected red blood cells. The lipid nanoparticles allowed the DNA to efficiently cross the lipid bilayers and enabled nuclear targeting of both linear as well as circular plasmid DNA molecules.…”

Section: Discussionmentioning

confidence: 99%

“…Natural cationic peptides such as protamine sulfate (PS) have been shown to increase gene transfection efficiency of both liposomal and polymeric nanoparticles1618. In addition, it is also known that sialylated glycoproteins of the RBC membrane contribute to its negatively charged surface, creating a repulsive electric zeta potential1920.…”

Section: Discussionmentioning

confidence: 99%

Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells

Gopalakrishnan

Kundu

Mandal

et al. 2013

Sci Rep

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Malaria threatens millions of people annually and is a burden to human health and economic development. Unfortunately in terms of disease control, no effective vaccines are available and the efficacy of treatment is limited by drug resistance. Genetic manipulation in Plasmodium falciparum is hampered due to the absence of robust methods for genetic analyses. Electroporation-based transfection methods have allowed the study of gene function in P. falciparum, with low efficiency. A lipid nanoparticle was developed that allowed nuclear targeting of pDNA with increased efficiency in reporter assay, compared to traditional electroporation method. This method has for the first time, facilitated transfection using both circular and linear DNA in P. falciparum thereby serving as an alternative to electroporation with an increase in transfection efficiency. Availability of a robust method for functional genomic studies in these organisms may be a catalyst for discovery of novel targets for developing drugs and vaccines.

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“…It has been reported that the use of natural cationic peptides such as PS can improve both in-vitro and in-vivo gene transfection efficiency of both liposomal and polymeric nanoparticles. [27,28,31] It is also known that cell membranes are negatively charged (e.g. the zeta-potential of Kupffer cells in liver has a negative charge density of approximately -4 mV).…”

Section: In-vitro Transfection Studiesmentioning

confidence: 99%

Development of nanosomes using high-pressure homogenization for gene therapy

Kundu

Hazari

Chinta

et al. 2010

Journal of Pharmacy and Pharmacology

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To conclude, these results indicate that condensing pDNA with PS followed by subsequent complexation with low-concentration nanosomes generated from HPH can produce a pDNA nanosome formulation that will boost transfection efficiency, while minimizing cytotoxicity. This new technology appears to be an efficient tool for future commercial or large-scale manufacture of DNA delivery systems for gene therapy.

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Encapsulation of protamine sulphate compacted DNA in polylactide and polylactide–co-glycolide microparticles

Cited by 36 publications

References 13 publications

Inhibitory efficacy of hypoxia-inducible factor 1α short hairpin RNA plasmid DNA-loaded poly (D, L-lactide-co-glycolide) nanoparticles on choroidal neovascularization in a laser-induced rat model

Inhibitory efficacy of hypoxia-inducible factor 1α short hairpin RNA plasmid DNA-loaded poly (D, L-lactide-co-glycolide) nanoparticles on choroidal neovascularization in a laser-induced rat model

Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells

Development of nanosomes using high-pressure homogenization for gene therapy

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