Endamoeba Histolytica and Other Intestinal Protozoa in 1,060 College Freshmen

Wenrich, D. H.; Stabler, Robert M.; Arnett, John H.

doi:10.4269/ajtmh.1935.s1-15.331

Cited by 23 publications

(5 citation statements)

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“…Dientamoeba trophozoites are typically binucleate, with up to 20% of forms being uninucleate (2), although this percentage can vary considerably (53). Nuclear pleomorphism is quite common, with the nucleus size varying in relation to the rest of the cell (55). In permanently stained smears, peripheral chromatin is absent, and the nuclear membrane is delicate.…”

Section: Morphological Characteristics Trophozoitesmentioning

confidence: 99%

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Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

et al. 2016

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SUMMARYDientamoeba fragilisis a protozoan parasite of the human bowel, commonly reported throughout the world in association with gastrointestinal symptoms. Despite its initial discovery over 100 years ago, arguably, we know less about this peculiar organism than any other pathogenic or potentially pathogenic protozoan that infects humans. The details of its life cycle and mode of transmission are not completely known, and its potential as a human pathogen is debated within the scientific community. Recently, several major advances have been made with respect to this organism's life cycle and molecular biology. While many questions remain unanswered, these and other recent advances have given rise to some intriguing new leads, which will pave the way for future research. This review encompasses a large body of knowledge generated on various aspects ofD. fragilisover the last century, together with an update on the most recent developments. This includes an update on the latest diagnostic techniques and treatments, the clinical aspects of dientamoebiasis, the development of an animal model, the description of aD. fragiliscyst stage, and the sequencing of the firstD. fragilistranscriptome.

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Section: Morphological Characteristics Trophozoitesmentioning

confidence: 99%

“…Wenrich et al (55) reported the detection of D. fragilis in 4.3% of stool specimens from 1,060 university students in the United States. Diarrhea and abdominal pain were the major symptoms recorded.…”

Section: Clinical Aspects and Epidemiologymentioning

confidence: 99%

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

et al. 2016

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“…Abdominal pain, diarrhea, abnormal stools, loss of appetite, and weight loss are the common symptoms that have been described previously (22). Wenrich et al (21) reported that there is a higher incidence of gastrointestinal disorders among college students harboring D. fragilis than among those infected with Entamoeba histolytica. Sapero (15) found that 27.3% of the patients infected with D. fragilis had symptoms, and Steinitz et al (18) reported symptoms in 15.1% of infected individuals.…”

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confidence: 93%

Susceptibility testing of Dientamoeba fragilis ATCC 30948 with iodoquinol, paromomycin, tetracycline, and metronidazole

Chan

Guan

Mackenzie

et al. 1994

Antimicrob Agents Chemother

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Susceptibility testing was performed on Dientamoebafragilis ATCC 30948 in a dixenic culture with Kkebsiella pneumoniae and Bacteroides vulgatus. D. fragilis was cocultured with the bacteria in TYGM-9 medium (ATCC medium 1171). The activities of antiparasitic drugs were assessed by counting viable D. fragilis trophozoites with a hemacytometer by trypan blue exclusion. The minimal amebicidal concentrations of the following four drugs were determined: iodoquinol at 128 ,ug/ml, paromomycin at 16 ,ug/ml, tetracycline (questionably) at 32 ,ug/ml, and metronidazole at 32 ,ug/ml.

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“…Dientamoeba fragilis is a protozoan parasite of the human large intestine. A pathogenic role for D. fragilis has been suggested (Wenrich et al ., 1935 ; Sapero, 1939 ; Girginkardesler et al ., 2003 ; Johnson et al ., 2004 ; Stark et al ., 2010 ; Barratt et al ., 2011a ); however, there is lack of conclusive evidence of its virulence and the mechanisms involved. Although first described almost a century ago by Jepps & Dobell ( 1918 ), little is known about D. fragilis transmission modes (Barratt et al ., 2011b ).…”

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confidence: 99%

Dientamoeba fragilisDNA detection inEnterobius vermiculariseggs

et al. 2013

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Dientamoeba fragilis is an intestinal protozoan suspected of causing gastrointestinal symptoms, and its mode of transmission is unknown, although first described almost a century ago. A hypothesis is that Enterobius vermicularis is a vector for D. fragilis, and recently, D. fragilis DNA was detected within surface-sterilized eggs of E. vermicularis. Using real-time PCR, we detected D. fragilis DNA in 18 (85%) of 21 samples of E. vermicularis eggs collected from patients harbouring D. fragilis in faeces. This finding supports the hypothesis that E. vermicularis may have an important role in the transmission of D. fragilis.This paper describes a protocol to wash and surface-sterilize E. vermicularis eggs, with the aim of showing presence of both E. vermicularis and D. fragilis specific DNA within, and the results from 20 co-infected patients. The study has merit as a confirmatory study of the trials by Röser et al. (2013), and includes improvements of the protocol.

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Endamoeba Histolytica and Other Intestinal Protozoa in 1,060 College Freshmen

Cited by 23 publications

References 0 publications

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

Susceptibility testing of Dientamoeba fragilis ATCC 30948 with iodoquinol, paromomycin, tetracycline, and metronidazole

Dientamoeba fragilisDNA detection inEnterobius vermiculariseggs

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