Endemicity and inter-city spread of Burkholderia cepacia genomovar III in cystic fibrosis

Chen, Janet S.; Witzmann, Kimberly; Spilker, Theodore; Fink, Robert; LiPuma, John J.

doi:10.1067/mpd.2001.118430

Cited by 115 publications

(149 citation statements)

References 24 publications

Supporting

Mentioning

146

Contrasting

Order By: Relevance

“…Hence, understanding the risk posed by the epidemic strains can still be useful for implementing infection control policies in centres that experience such problems. It is worth noting that identification of transmissible strains should be based on genotyping methods and not be confined to markers of transmissibility as they are not absolute markers for epidemic strains (Chen et al, 2001;McDowell et al, 2004). Lack of these markers for definitive identification was also indicated during our study where one sporadic isolate with a unique banding profile (i.e.…”

Section: Infection Control In Bcc-positive Patientsmentioning

confidence: 92%

“…For example, strains from the cable-pilus-encoding ET12 lineage have been responsible for epidemic outbreaks in a Toronto CF centre, and subsequently were spread to the UK (Johnson et al, 1994;Sun et al, 1995). Other transmissible strains within B. cenocepacia but distinct from the ET12 lineage have been identified in Canada [strain types designated RAPD 01, 04 and 06 (Speert et al, 2002); belonging to recA-derived subgroup IIIA] and in the USA [PHDC strain (Chen et al, 2001) and Midwest clone (LiPuma et al, 1988); belonging to recA group IIIB]. Recently published data on PHDC and its presence in Europe indicate that transatlantic spread of the Bcc is not a feature exclusive to the ET12 lineage (Coenye et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Widespread clone of Burkholderia cenocepacia in cystic fibrosis patients in the Czech Republic

Dřevı́nek

Vosahlikova

Cinek

et al. 2005

Journal of Medical Microbiology

View full text Add to dashboard Cite

The morbidity and mortality rates in patients with cystic fibrosis (CF) are significantly affected by infections with Burkholderia cepacia complex. In a Czech CF Centre, the prevalence of the infection reached up to 30 %, with the majority of patients found to be infected with Burkholderia cenocepacia (formerly genomovar III of the Burkholderia cepacia complex). Since B. cenocepacia is associated with patient-to-patient transmission and epidemic outbreaks among CF patients, this study sought to examine the epidemiological relatedness between the Czech isolates belonging to the genomovar-homogeneous group. Eighty-three clinical isolates recovered from 67 CF patients were analysed using a random amplified polymorphic DNA (RAPD) assay and macrorestriction typing (SpeI and XbaI) followed by PFGE. A single predominant banding pattern shared by multiple isolates was detected, although SpeI-generated PFGE results yielded a higher rate of inter-pattern variability in comparison to the more uniform RAPD and XbaI-generated PFGE results for this clone. Both typing systems also showed that only three out of 67 patients harboured strains distinct from the major strain type. The dominant clone was characterized by PCR positivity for the B. cepacia epidemic strain marker, PCR negativity for the cable pilin subunit gene and close genetic relatedness to the epidemic strain of RAPD 01 type previously identified in Canada.

show abstract

Section: Infection Control In Bcc-positive Patientsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Widespread clone of Burkholderia cenocepacia in cystic fibrosis patients in the Czech Republic

Dřevı́nek

Vosahlikova

Cinek

et al. 2005

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…A dendrogram was derived from the UPGMA linkage of correlation coefficients between the BOX-PCR fingerprint patterns from Bcc isolates. Data for reference strains were available from previous studies (Chen et al, 2001;Coenye et al, 2002Coenye et al, , 2004LiPuma et al, 2002) or were unpublished (J. J. LiPuma).…”

Section: Methodsmentioning

confidence: 99%

Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex

Vanlaere

LiPuma

Baldwin

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

223

143

View full text Add to dashboard Cite

The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach, The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA-DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3(T) =LMG 24064(T) =CCUG 54555(T)), Burkholderia diffusa sp. nov. (type strain AU1075(T) =LMG 24065(T) =CCUG 54558(T)), Burkholderia arboris sp. nov. (type strain ES0263A(T) =LMG 24066(T) =CCUG 54561(T)), Burkholderia seminalis sp. nov. (type strain AU0475(T) =LMG 24067(T) =CCUG 54564 (T)) and Burkholderia metallica sp. nov. (type strain AU0553(T) =LMG 24068(T) =CCUG 54567(T)). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc

show abstract

“…Isolate 225 lost viability during subsequent cultivation. To check clonality using randomly amplified polymorphic DNA (RAPD; Chen et al, 2001), DNA from each isolate was prepared by alkaline lysis (Coenye et al, 2002) and the DNA solutions were stored at 220 u C. Fingerprinting using RAPD was performed with primers RAPD-270 (59-TGCGCGCGGG-39) and RAPD-272 (59-AGCGGGCCAA-39), as described previously (Mahenthiralingam et al, 1996). Isolates 215 T and 194B were found to be non-clonal strains (data not shown).…”

mentioning

confidence: 99%

Weissella ghanensis sp. nov., isolated from a Ghanaian cocoa fermentation

Bruyne

Camu

Lefebvre

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

During a study on lactic acid bacteria (and their species diversity) in spontaneous heap fermentations of Ghanaian cocoa beans, two strains, designated 215 T and 194B, were isolated. A phylogenetic analysis based on 16S rRNA gene sequences demonstrated that these strains represented a distinct lineage close to the genus Weissella and showing only 92.1 % 16S rRNA gene sequence similarity with respect to their closest neighbour, Weissella soli LMG 20113 T . Whole-cell protein electrophoresis, fluorescent amplified fragment length polymorphism fingerprinting of whole genomes and physiological and biochemical tests confirmed the unique taxonomic position of the two novel isolates. On the basis of the results of the morphological and biochemical tests and 16S rRNA gene sequence analysis, strains 215 T and 194B represent the most peripheral lineage of the genus Weissella, for which we propose the name Weissella ghanensis sp. nov. The type strain is 215 T (5LMG 24286 T 5DSM 19935 T ).The genus Weissella was created by Collins et al. (1993), in the course of a phylogenetic analysis of some unknown Leuconostoc-like lactic acid bacteria, to accommodate five heterofermentative Lactobacillus species and a species formerly classified as Leuconostoc paramesenteroides. Novel species of the genus Weissella have mainly been isolated from fermented foods: Weissella hellenica strains were isolated from fermented sausages (Collins et al., 1993), Weissella kimchii (a later heterotypic synonym of Weissella cibaria) (Ennahar & Cai, 2004) and Weissella koreensis strains were isolated from kimchi (Choi et al., 2002;Lee et al., 2002), and Weissella thailandensis strains were isolated from fermented fish (Tanasupawat et al., 2000). The exceptions are Weissella soli strains, which were isolated from soil (Magnusson et al., 2002), and some W. cibaria strains that were detected in both fermented food and clinical samples (Björkroth et al., 2002).The population dynamics of spontaneous heap fermentations of cocoa beans in Ghana during the main cropping season of 2005 (October-December) were studied by Camu et al. (2007). The levels of lactic acid bacteria species diversity of different fermentation heaps and different fermentation times were signalled by the relative intensities of bands obtained using a culture-independent technique, namely 16S rRNA gene sequence-based PCRdenaturing gradient gel electrophoresis. This analysis detected an unidentified bacterium that appeared in five of the seven heap fermentations studied, from the beginning of the fermentation up to 48 h after the start of the fermentation process. The 16S rRNA gene sequence of this bacterium was determined. Only three isolates (215 T , 194B and 225) that had 16S rRNA gene sequences identical to that of the unidentified bacterium were obtained (and subsequently cultivated) from the same fermenting cocoa bean heap in New Tafo, Ghana (Camu et al., 2007). Isolate 225 lost viability during subsequent cultivation. To check clonality using randomly amplified polymorphic DNA (RAPD; ...

show abstract

Endemicity and inter-city spread of Burkholderia cepacia genomovar III in cystic fibrosis

Cited by 115 publications

References 24 publications

Widespread clone of Burkholderia cenocepacia in cystic fibrosis patients in the Czech Republic

Widespread clone of Burkholderia cenocepacia in cystic fibrosis patients in the Czech Republic

Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex

Weissella ghanensis sp. nov., isolated from a Ghanaian cocoa fermentation

Contact Info

Product

Resources

About