Endocarditis caused by Enterococcus faecalis with highlevel resistance to aminoglycosides: failure of ampicillin and ceftriaxone combined therapy

Venditti, Mario; Brandimarte, C; Capone, Alessandro; Cassone, Marco; M, Galiè; Tarasi, A.; Tarasi, David

doi:10.1111/j.1469-0691.1997.tb00314.x

Cited by 7 publications

(5 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, there is no known effective medical treatment for patients with endocarditis in whom the infecting strain of E. faecalis is susceptible to ampicillin but highly resistant to aminoglycosides. Recently, Venditti et al (44) described a patient with endocarditis due to HLRAg E. faecalis for whom treatment with ampicillin plus ceftriaxone failed. One possible explanation for this failure is that ceftriaxone had to be stopped after 3 weeks because of fever related to the administration of the drug.…”

Section: Discussionmentioning

confidence: 99%

Efficacy of Ampicillin plus Ceftriaxone in Treatment of Experimental Endocarditis Due to Enterococcus faecalis Strains Highly Resistant to Aminoglycosides

Gavaldá

Torres

Tenorio

et al. 1999

Antimicrob Agents Chemother

118

View full text Add to dashboard Cite

The purpose of this work was to evaluate the in vitro possibilities of ampicillin-ceftriaxone combinations for 10 Enterococcus faecalis strains with high-level resistance to aminoglycosides (HLRAg) and to assess the efficacy of ampicillin plus ceftriaxone, both administered with humanlike pharmacokinetics, for the treatment of experimental endocarditis due to HLRAg E. faecalis. A reduction of 1 to 4 dilutions in MICs of ampicillin was obtained when ampicillin was combined with a fixed subinhibitory ceftriaxone concentration of 4 μg/ml. This potentiating effect was also observed by the double disk method with all 10 strains. Time-kill studies performed with 1 and 2 μg of ampicillin alone per ml or in combination with 5, 10, 20, 40, and 60 μg of ceftriaxone per ml showed a ≥2 log10 reduction in CFU per milliliter with respect to ampicillin alone and to the initial inoculum for all 10E. faecalis strains studied. This effect was obtained for seven strains with the combination of 2 μg of ampicillin per ml plus 10 μg of ceftriaxone per ml and for six strains with 5 μg of ceftriaxone per ml. Animals with catheter-induced endocarditis were infected intravenously with 108 CFU of E. faecalis V48 or 105 CFU of E. faecalisV45 and were treated for 3 days with humanlike pharmacokinetics of 2 g of ampicillin every 4 h, alone or combined with 2 g of ceftriaxone every 12 h. The levels in serum and the pharmacokinetic parameters of the humanlike pharmacokinetics of ampicillin or ceftriaxone in rabbits were similar to those found in humans treated with 2 g of ampicillin or ceftriaxone intravenously. Results of the therapy for experimental endocarditis caused by E. faecalis V48 or V45 showed that the residual bacterial titers in aortic valve vegetations were significantly lower in the animals treated with the combinations of ampicillin plus ceftriaxone than in those treated with ampicillin alone (P < 0.001). The combination of ampicillin and ceftriaxone showed in vitro and in vivo synergism against HLRAgE. faecalis.

show abstract

Section: Discussionmentioning

confidence: 99%

Efficacy of Ampicillin plus Ceftriaxone in Treatment of Experimental Endocarditis Due to Enterococcus faecalis Strains Highly Resistant to Aminoglycosides

Gavaldá

Torres

Tenorio

et al. 1999

Antimicrob Agents Chemother

118

View full text Add to dashboard Cite

show abstract

“…Only one strain appeared to be tolerant to ampicillin. It is widely known that most enterococci are less likely killed at elevated concentrations of beta-lactam antibiotics [ 5 , 9 , 19 ]. Thus, the bactericidal effect of ampicillin in these experiments may have been due to the tested ampicillin concentrations which were very close to the MICs.…”

Section: Discussionmentioning

confidence: 99%

In vitro Antimicrobial Activity of Ampicillin-Ceftriaxone and Ampicillin-Ertapenem Combinations Against Clinical Isolates of Enterococcus faecalis with High Levels of Aminoglycoside Resistance

Pasticci

Mencacci²,

Moretti³

et al. 2008

TOMICROJ

View full text Add to dashboard Cite

This paper reports on the in vitro antimicrobial activity of ampicillin-ceftriaxone and ampicillin-ertapenem combinations against five strains of E. faecalis with high-level aminoglycoside resistance recovered from blood of septicemic patients. Double disk diffusion test and time killing curves were used. A bacteriostatic synergistic effect between ampicillin and ceftriaxone was detected using the disk diffusion assay for three of the five enterococcal strains studied. With the same three isolates enhanced bactericidal activity was also observed using time killing experiments. Overall, for these three strains, after 24 hr of contact, a decrease 2 log 10 from the initial bacterial inoculum was registered with most ampicillin-ceftriaxone combinations, reaching with some of them a colony reduction 3 log 10 . This bactericidal interaction was negatively influenced increasing the bacterial inoculum. In all five isolates neither a bacteriostatic nor a bactericidal cooperation was observed for ampicillin combined with 2 mg/l of ertapenem.This investigation broadened the evidence of antimicrobial synergism in vitro between ampicillin and ceftriaxone in selected strains of Enterococcus faecalis with high-level aminoglycoside resistance.

show abstract

“…10 The third case report, describing a treatment failure, involved a patient with HLAR E faecalis who relapsed after several courses of therapy and was then placed on ampicillin and ceftriaxone. 12 Ceftriaxone was stopped after 21 days because of an infusion-associated reaction, and the ampicillin was continued up to 8 weeks. In this case, valve replacement was not feasible; the infection persisted, and the patient was subsequently prescribed suppressive therapy.…”

Section: Dual β-Lactam Synergy Against E Faecalismentioning

confidence: 99%

“…In this case, valve replacement was not feasible; the infection persisted, and the patient was subsequently prescribed suppressive therapy. 12…”

Section: Dual β-Lactam Synergy Against E Faecalismentioning

confidence: 99%

See 1 more Smart Citation

Combination of Ceftriaxone and Ampicillin for the Treatment of Enterococcal Endocarditis: A Qualitative Systematic Review

2017

View full text Add to dashboard Cite

show abstract

Endocarditis caused by Enterococcus faecalis with highlevel resistance to aminoglycosides: failure of ampicillin and ceftriaxone combined therapy

Abstract: S h o r t C o m m u n i c a t i o n s a n d L e t t e r s t o t h e E d i t o r s 577

Cited by 7 publications

References 20 publications

Efficacy of Ampicillin plus Ceftriaxone in Treatment of Experimental Endocarditis Due to Enterococcus faecalis Strains Highly Resistant to Aminoglycosides

Efficacy of Ampicillin plus Ceftriaxone in Treatment of Experimental Endocarditis Due to Enterococcus faecalis Strains Highly Resistant to Aminoglycosides

In vitro Antimicrobial Activity of Ampicillin-Ceftriaxone and Ampicillin-Ertapenem Combinations Against Clinical Isolates of Enterococcus faecalis with High Levels of Aminoglycoside Resistance

Combination of Ceftriaxone and Ampicillin for the Treatment of Enterococcal Endocarditis: A Qualitative Systematic Review

Contact Info

Product

Resources

About