In vitro Antimicrobial Activity of Ampicillin-Ceftriaxone and Ampicillin-Ertapenem Combinations Against Clinical Isolates of Enterococcus faecalis with High Levels of Aminoglycoside Resistance

Pasticci, Maria Bruna; Mencacci, Antonella; Moretti, Amedeo; Palladino, Nicola; Lapalorcia, Luigi Maria; Bistoni, Francesco; Baldelli, Franco

doi:10.2174/1874285800802010079

Cited by 17 publications

(5 citation statements)

References 21 publications

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“…In in vitro and animal model studies, similar synergistic bactericidal activity has been shown with other cell wall active combination therapies, including ceftriaxone-fosfomycin 133 and ampicillin-imipenem, 134 but not with ampicillin-ertapenem. 135 …”

Section: Managementmentioning

confidence: 99%

Intrinsic and acquired resistance mechanisms in enterococcus

Hollenbeck

Rice²

2012

Virulence

630

530

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Enterococci have the potential for resistance to virtually all clinically useful antibiotics. Their emergence as important nosocomial pathogens has coincided with increased expression of antimicrobial resistance by members of the genus. The mechanisms underlying antibiotic resistance in enterococci may be intrinsic to the species or acquired through mutation of intrinsic genes or horizontal exchange of genetic material encoding resistance determinants. This paper reviews the antibiotic resistance mechanisms in Enterococcus faecium and Enterococcus faecalis and discusses treatment options.

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Section: Managementmentioning

confidence: 99%

Intrinsic and acquired resistance mechanisms in enterococcus

Hollenbeck

Rice²

2012

Virulence

630

530

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“…Enterococcus faecalis is one of the main nosocomial pathogens ( Horner et al , 2005 ) which may be resistant to a wide range of antimicrobial agents, causing several diseases. These bacteria inhabit the gastrointestinal and genitourinary tracts and have been related to infectious endocarditis and surgical wound infection ( Pasticci et al , 2008 ). In the oral cavity, E. faecalis is likely to be found in caries lesions, in periodontal diseases ( Souto et al , 2006 ), and mainly in endodontic infections ( Hancock et al , 2001 , Duggan and Sedgley, 2007 ), in which pulp may become infected via dentinal tubules, through carious lesions, and via periodontal disease.…”

Section: Introductionmentioning

confidence: 99%

In vitro activity of Amazon plant extracts against Enterococcus faecalis

Castilho¹,

Silva²,

Saraceni³

et al. 2014

Braz. J. Microbiol.

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Previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from Brazilian forests’ plants. In the present study, these extracts were subjected to microdilution broth assay (MDBA) and disk diffusion assay (DDA) using planktonic Enterococcus faecalis ATCC® 29212™ and were submitted to phytochemical analysis in TLC and HPLC. Three extracts obtained from Ipomoea alba (MIC < 40 μg/mL), Diclinanona calycina (MIC ≤ 40 μg/mL) and Moronobea coccinea (40 < MIC < 80 μg/mL; MBC = 80 μg/mL) showed significant bactericidal activity in the MDBA and four extracts obtained from I. alba (14.04 ± 0.55 mm diameter) S. globulifera (14.43 ± 0.33 mm and 12.18 ± 0.28 mm diameter) and Connarus ruber var. ruber (13.13 ± 0.18 mm diameter) were active in DDA. Residues H2O obtained from Psidium densicomum (mean of 16.78 mm diameter) and from Stryphnodendron pulcherrimum (mean of 15.97 mm diameter) have shown an improved antibacterial activity after fractionation if compared to that obtained from the respective crude extracts. Antioxidant activity was observed in some residues of the active extracts. TLC analysis showed that phenolic compounds are likely to be found in active extracts. Three molecules were isolated from S. globulifera and were identified by 13C NMR lupeol, α-amyrin and 3β-hydroxyglutin-5-ene. The present chemical and biological findings suggest that these extracts are a potential source of new anti-Enterococcus compounds to be introduced in endodontic therapy.

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“…Studying the interactions between ceftriaxone and ampicillin by the double-disk test and the time-kill curves, Pasticci et al [24] have observed an increase in the antimicrobial activity of both molecules in 3 out of 5 strains tested. …”

Section: Discussionmentioning

confidence: 99%

In vitro Activity Effects of Twelve Antibiotics Alone and in Association against Twenty-Seven <i>Enterococcus faecalis</i> Strains Isolated from Italian Patients with Infective Endocarditis: High in vitro Synergistic Effect of the Association Ceftriaxone-Fosfomycin

et al. 2011

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Background: In 2004–2008, the epidemiological and clinical Infective Endocarditis Study Group (SEI) evaluated 852 cases of infective endocarditis. Staphylococcus aureus was the main involved pathogen (24.5%) and Enterococcus faecalis etiology was described in 11% of the cases. The aim of this study was to evaluate the in vitro activity of 12 antibiotics alone and in association against 27 strains of E. faecalis isolated from blood cultures of patients with infective endocarditis. Results: The results showed high in vitro activity of tigecycline, daptomycin and linezolid. A high synergistic effect was obtained with the association ceftriaxone-fosfomycin [fractional inhibitory concentration (FIC)₅₀ = 0.34, FIC₉₀ = 0.78]. Furthermore, ceftriaxone plus ampicillin presented additive results (FIC₅₀ = 0.66, FIC₉₀ = 1.00), and ceftriaxone plus fosfomycin and ceftriaxone plus ampicillin were significantly more active in vitro than each drug alone. The efficacy of ceftriaxone plus fosfomycin was confirmed by the association testing using the broth dilution technique. Conclusion: Fosfomycin seems particularly significant and its association with ceftriaxone could be considered as a useful therapeutic option in medical treatment of E. faecalis infective endocarditis.

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In vitro Antimicrobial Activity of Ampicillin-Ceftriaxone and Ampicillin-Ertapenem Combinations Against Clinical Isolates of Enterococcus faecalis with High Levels of Aminoglycoside Resistance

Cited by 17 publications

References 21 publications

Intrinsic and acquired resistance mechanisms in enterococcus

Intrinsic and acquired resistance mechanisms in enterococcus

In vitro activity of Amazon plant extracts against Enterococcus faecalis

In vitro Activity Effects of Twelve Antibiotics Alone and in Association against Twenty-Seven <i>Enterococcus faecalis</i> Strains Isolated from Italian Patients with Infective Endocarditis: High in vitro Synergistic Effect of the Association Ceftriaxone-Fosfomycin

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