In vitro activity of Amazon plant extracts against Enterococcus faecalis

Abstract

Previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from Brazilian forests’ plants. In the present study, these extracts were subjected to microdilution broth assay (MDBA) and disk diffusion assay (DDA) using planktonic Enterococcus faecalis ATCC® 29212™ and were submitted to phytochemical analysis in TLC and HPLC. Three extracts obtained from Ipomoea alba (MIC < 40 μg/mL), Diclinanona calycina (MIC ≤ 40 μg/mL) and Moronobea coccinea (40 < MIC < 80 μg/mL; MBC = 80 μg/mL) showed significant bactericidal activity in the MDBA and four extracts obtained from I. alba (14.04 ± 0.55 mm diameter) S. globulifera (14.43 ± 0.33 mm and 12.18 ± 0.28 mm diameter) and Connarus ruber var. ruber (13.13 ± 0.18 mm diameter) were active in DDA. Residues H2O obtained from Psidium densicomum (mean of 16.78 mm diameter) and from Stryphnodendron pulcherrimum (mean of 15.97 mm diameter) have shown an improved antibacterial activity after fractionation if compared to that obtained from the respective crude extracts. Antioxidant activity was observed in some residues of the active extracts. TLC analysis showed that phenolic compounds are likely to be found in active extracts. Three molecules were isolated from S. globulifera and were identified by 13C NMR lupeol, α-amyrin and 3β-hydroxyglutin-5-ene. The present chemical and biological findings suggest that these extracts are a potential source of new anti-Enterococcus compounds to be introduced in endodontic therapy.

Comparative Microbicidal Efficacy of Fractionated Extracts from In Vitro and In Vivo Raised Cells of Tinosporacordifolia Against MDR Pathogens

“…Similarly to the MIC by serial dilution method, fractions of callus extracts showed better zone of inhibition (ZOI) by disc diffusion and food poisoning methods than fractions of leaf extracts. There are numerous reports in support to our findings (Srivastavaet al 2013;Castilho et al 2014;Kumariet al 2015;de Souza et al 2015). Several reports suggest that the in vitro shoot culture show maximum accumulation of bioactive compounds (Sharda et al 2007;Jain et al 2011).…”

Microbial Profile of the Ventriculum of Honey Bee (Apis mellifera ligustica Spinola, 1806) Fed with Veterinary Drugs, Dietary Supplements and Non-Protein Amino Acids

“…Even if an antimicrobial activity against Enterococcus spp. is reported for formic acid [ 39 , 40 ], probably the combination with oxalic acid in VM may modify or reduce the effect of formic acid. The growth of some bacteria is probably due to their ability to use organic acids as a nitrogen and energy source [ 41 , 42 ].…”

Mice behavioral phenotype changes after administration of Anani (Symphonia globulifera, clusiaceae), an alternative Latin American and African medicine

“…The previous reports relating the influence of alternative medicines over behavior have been done, such as to honey,[ 22 ] to the sino-japanese wakan-yaku,[ 23 ] to uncaria hook[ 24 ] and others. [ 25 26 ] Lupeol, β-amyrin and 3 β-hydroxyglutin-5-ene were previously isolated from S. globulifera ,[ 21 ] and molecules were described to occur in the species for the first time, then. Before that, nine polycyclic polyprenylated acylphloroglucinol compounds and oxidized derivatives having a slight antiplasmoidal activity were isolated from the root barks,[ 6 ] xanthones (ugaxanthone, barraxanthone, and gentisein), polyoxygenated xanthone (globulixanthone F), and biflavonoids (GB2 and manniflavanone GB3) were isolated from the stem bark, also, globulixanthone F were tested against a series of microorganisms.…”

“…Preparative TLC was used in the purification of some fractions from FrHEX, and one of the fractions was sent to carbon nuclear magnetic resonance analyses. Three compounds were previously isolated from the organic extract of S. globulifera and were identified[ 21 ] as lupeol (1), β -amyrin (2) and 3- β -hydroxyglutin-5-ene (3) [ Figure 2 ]. In the present work, oleanolic acid (4) was identified and is being described to occur in S. globulifera for the first time.…”