Endocrine and Exocrine Function of the Bovine Testes

Ryan, Peter L.

doi:10.1002/9781118833971.ch2

Cited by 2 publications

(1 citation statement)

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“…In that situation, low-level of SRY is inadequate to initiate testis differentiation because of insufficient SOX9 . Therefore, bipotential-gonad cells would not differentiate into sertoli cells and leydig cells which are vital for males’ cell differentiation and proliferation ( Rebourcet et al, 2014 ; Ryan, 2014 ). Anastomoses of two opposite-sex fetuses occur after the critical period of reproductive organ differentiation may lead to low-level of SRY ( Szczerbal et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Applying real-time quantitative PCR to diagnosis of freemartin in Holstein cattle by quantifyingSRYgene: a comparison experiment

Qiu

Shao

et al. 2018

PeerJ

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BackgroundFreemartinism generally occurs in female offspring of dizygotic twins in a mixed-sex pregnancy. Most bovine heterosexual twin females are freemartins. However, about 10% of bovine heterosexual twin females are fertile. Farmers mostly cull bovine fertile heterosexual twin females due to the lack of a practical diagnostic approach. Culling of such animals results in economic and genetic-material losses both for dairy and beef industry.MethodsIn this study, a comparative test, including qualitative detection of SRY gene by polymerase chain reaction (PCR), quantitative detection of relative content of SRY by real-time quantitative polymerase chain reaction (qPCR), and quantitative detection of H-Y antigen, was performed to establish the most accurate diagnosis for freemartin. Twelve Holstein heterosexual twin females were used in this study, while three normal Holstein bulls and three normal Holstein cows were used as a positive and negative control, respectively.ResultsPolymerase chain reaction results revealed that SRY gene were absent in three heterosexual twin females and only two of them were verified as fertile in later age. The qPCR results showed that relative content of SRY was more than 14.2% in freemartins and below 0.41% in fertile heterosexual twin females. The H-Y antigen test showed no significant numerical difference between freemartin and fertile heterosexual twin female.DiscussionOur results show that relative content of SRY quantified by qPCR is a better detection method for diagnosis of freemartin in Holstein cattle as compare to qualitative detection of SRY gene by PCR or quantitative detection of H-Y antigen. To the authors’ knowledge, this is the first time we applied qPCR to diagnosing freemartin by quantifying SRY gene and got relative SRY content of each freemartin and fertile heterosexual twin female. We concluded that low-level of SRY would not influence fertility of bovine heterosexual twin female.

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Section: Discussionmentioning

confidence: 99%