Endocrine and metabolic function in male Carioca High-conditioned Freezing rats

Mousovich-Neto, Felippe; Lourenço, Alexandre Lopes; Landeira-Fernández, J.; Costa, Valentina

doi:10.1016/j.physbeh.2015.01.028

Cited by 11 publications

(7 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No differences were found between CHF and CLF rats in the number of closed arm entries, suggesting that the anxiety-like profile of CHF animals was not attributable to locomotor impairment but rather to increases in aversion to the open arms. These behavioral differences between CHF and CLF rats in the EPM are consistent with previous studies from our laboratory and indicate that the conditioned freezing parameter that is used for our breeding program remained stable in a different threatening situation across different generations (Hassan et al, 2013, 2015; Dias et al, 2014; Mousovich-Neto et al, 2015). …”

Section: Discussionsupporting

confidence: 88%

Behavioral Effects of Systemic, Infralimbic and Prelimbic Injections of a Serotonin 5-HT2A Antagonist in Carioca High- and Low-Conditioned Freezing Rats

León

Castro-Gomes

Zárate-Guerrero

et al. 2017

Front. Behav. Neurosci.

Self Cite

View full text Add to dashboard Cite

The role of serotonin (5-hydroxytryptamine [5-HT]) and 5-HT2A receptors in anxiety has been extensively studied, mostly without considering individual differences in trait anxiety. Our laboratory developed two lines of animals that are bred for high and low freezing responses to contextual cues that are previously associated with footshock (Carioca High-conditioned Freezing [CHF] and Carioca Low-conditioned Freezing [CLF]). The present study investigated whether ketanserin, a preferential 5-HT2A receptor blocker, exerts distinct anxiety-like profiles in these two lines of animals. In the first experiment, the animals received a systemic injection of ketanserin and were exposed to the elevated plus maze (EPM). In the second experiment, these two lines of animals received microinjections of ketanserin in the infralimbic (IL) and prelimbic (PL) cortices and were exposed to either the EPM or a contextual fear conditioning paradigm. The two rat lines exhibited bidirectional effects on anxiety-like behavior in the EPM and opposite responses to ketanserin. Both systemic and intra-IL cortex injections of ketanserin exerted anxiolytic-like effects in CHF rats but anxiogenic-like effects in CLF rats. Microinjections of ketanserin in the PL cortex also exerted anxiolytic-like effects in CHF rats but had no effect in CLF rats. These results suggest that the behavioral effects of 5-HT2A receptor antagonism might depend on genetic variability associated with baseline reactions to threatening situations and 5-HT2A receptor expression in the IL and PL cortices.Highlights CHF and CLF rats are two bidirectional lines that are based on contextual fear conditioning.CHF rats have a more “anxious” phenotype than CLF rats in the EPM.The 5-HT2A receptor antagonist ketanserin had opposite behavioral effects in CHF and CLF rats.Systemic and IL injections either decreased (CHF) or increased (CLF) anxiety-like behavior.PL injections either decreased (CHF) anxiety-like behavior or had no effect (CLF).

show abstract

Section: Discussionsupporting

confidence: 88%

Behavioral Effects of Systemic, Infralimbic and Prelimbic Injections of a Serotonin 5-HT2A Antagonist in Carioca High- and Low-Conditioned Freezing Rats

León

Castro-Gomes

Zárate-Guerrero

et al. 2017

Front. Behav. Neurosci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Consequently, GAD patients have been found to have elevated cortisol levels (Lenze et al, 2011 ). Again, CHF rats were equally selective for this parameter as reported by Mousovich-Neto et al ( 2015 ). These authors showed increased neuroendocrine responses (i.e., increased serum corticosterone) in CHF rats comparted to control animals.…”

Section: Chf Rat Line As a Model Of Gadsupporting

confidence: 80%

An animal model of trait anxiety: Carioca high freezing rats as a model of generalized anxiety disorder

Cruz,

Castro-Gomes,

Landeira-Fernandez

2024

Personality Neuroscience

Self Cite

View full text Add to dashboard Cite

Despite being one of the main components of anxiety and playing a pivotal role in how an individual perceives and copes with anxiogenic situations or responds to a given treatment, trait anxiety is paradoxically omitted in most animal models of anxiety. This is problematic and particularly more concerning in models that are used to screen drugs and other treatments for specific anxiety disorders and to investigate their neurobiological mechanisms. Our group has been engaged in the search for specific anxiety-related traits in animal models of anxiety. We developed two new lines of rats with strong phenotypic divergence for high (Carioca High-conditioned Freezing [CHF]) and low (Carioca Low-conditioned Freezing [CLF]) trait anxiety as expressed in the contextual fear conditioning paradigm. Here, we summarize key behavioral, pharmacological, physiological, and neurobiological differences in one these lines, the CHF rat line, relative to randomized-cross controls and discuss how far they represent a valid and reliable animal model of generalized anxiety disorder and so high trait anxiety.

show abstract

“…Past studies have characterized the Carioca line as a reliable rat model of innate anxiety (for a review, see Gomes et al, 2011). Apart from context fear conditioning, CHF rats display more anxious like behaviors than CLF rats in the elevated plus maze, less social interactions than CTL animals, and higher plasma corticosterone concentrations compared to both CLF and CTL animals (Dias et al, 2009;Salviano et al, 2014;Mousovich-Neto et al, 2015). Similar result patterns were not observed for behavioral tests measuring depression traits and cognitive skills.…”

Section: Discussionmentioning

confidence: 52%

Cued Fear Conditioning in Carioca High- and Low-Conditioned Freezing Rats

Macêdo-Souza

Maisonnette

Filgueiras

et al. 2020

Front. Behav. Neurosci.

Self Cite

View full text Add to dashboard Cite

Anxiety disorders (AD) comprise a broad range of psychiatric conditions, including general anxiety (GAD) and specific phobias. For the last decades, the use of animal models of anxiety has offered important insights into the understanding of the association between these psychopathologies. Here, we investigate whether Carioca high-and low-conditioned freezing rats (CHF and CLF, respectively), a GAD animal model of anxiety, show similar high-and low-freezing behavioral phenotypes for cued auditory fear conditioning. Adult CHF (n = 16), CLF (n = 16) and normal age-matched Wistar rats (control, CTL, n = 16) were tested in a classical auditory-cued fear conditioning paradigm over 3 days (Tone + Shock and Tone only groups, n = 8 per treatment). Freezing responses were measured and used as evidence of fear conditioning. Overall, both CHF and CLF rats, as well as CTL animals displayed fear conditioning to the auditory CS. However, CLF animals showed a rapid extinction to the auditory conditioned stimulus compared to CHF and CTL rats. We discuss these findings in the context of the behavioral and neuronal differences observed in rodent lines of high and low anxiety traits.

show abstract

Endocrine and metabolic function in male Carioca High-conditioned Freezing rats

Cited by 11 publications

References 42 publications

Behavioral Effects of Systemic, Infralimbic and Prelimbic Injections of a Serotonin 5-HT2A Antagonist in Carioca High- and Low-Conditioned Freezing Rats

Behavioral Effects of Systemic, Infralimbic and Prelimbic Injections of a Serotonin 5-HT2A Antagonist in Carioca High- and Low-Conditioned Freezing Rats

An animal model of trait anxiety: Carioca high freezing rats as a model of generalized anxiety disorder

Cued Fear Conditioning in Carioca High- and Low-Conditioned Freezing Rats

Contact Info

Product

Resources

About