Endocrine disruption: molecular interactions of chlorpyrifos and its degradation products with estrogen receptor

Hazarika, Jnyandeep; Ganguly, Mausumi; Borgohain, Gargi; Baruah, Indrani; Sarma, Shruti; Bhuyan, Pranjal; Mahanta, Rita

doi:10.1007/s11224-020-01562-4

Cited by 11 publications

(3 citation statements)

References 36 publications

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“…One of the limitations of our study is that we only examined the effects of CPF in male mice. It is known that CPF interacts with sex hormones 63 and steroid hormone receptors 64 . In terms of the neurotoxic effects, males are more susceptible to organophosphates than females 65 .…”

Section: Discussionmentioning

confidence: 99%

The pesticide chlorpyrifos promotes obesity by inhibiting diet-induced thermogenesis in brown adipose tissue

et al. 2021

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Obesity results from a caloric imbalance between energy intake, absorption and expenditure. In both rodents and humans, diet-induced thermogenesis contributes to energy expenditure and involves the activation of brown adipose tissue (BAT). We hypothesize that environmental toxicants commonly used as food additives or pesticides might reduce BAT thermogenesis through suppression of uncoupling protein 1 (UCP1) and this may contribute to the development of obesity. Using a step-wise screening approach, we discover that the organophosphate insecticide chlorpyrifos suppresses UCP1 and mitochondrial respiration in BAT at concentrations as low as 1 pM. In mice housed at thermoneutrality and fed a high-fat diet, chlorpyrifos impairs BAT mitochondrial function and diet-induced thermogenesis, promoting greater obesity, non-alcoholic fatty liver disease (NAFLD) and insulin resistance. This is associated with reductions in cAMP; activation of p38MAPK and AMPK; protein kinases critical for maintaining UCP1 and mitophagy, respectively in BAT. These data indicate that the commonly used pesticide chlorpyrifos, suppresses diet-induced thermogenesis and the activation of BAT, suggesting its use may contribute to the obesity epidemic.

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Section: Discussionmentioning

confidence: 99%

The pesticide chlorpyrifos promotes obesity by inhibiting diet-induced thermogenesis in brown adipose tissue

et al. 2021

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“…Below these levels, consumption is considered safe. However, for some pesticides and their transformation products, xenoestrogenic activity has been reported frequently [4][5][6][7][8][9]. Xenoestrogens can interact with the human estrogen receptors hERα and hERβ and thus disturb the normal reproductive process [10], leading, for example, to low sperm count and adverse pregnancy outcomes [11].…”

Section: Introductionmentioning

confidence: 99%

Non-target estrogenic screening of 60 pesticides, six plant protection products, and tomato, grape, and wine samples by planar chromatography combined with the planar yeast estrogen screen bioassay

2023

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For non-target residue analysis of xenoestrogens in food, sophisticated chromatographic–mass spectrometric techniques lack in biological effect detection. Various in vitro assays providing sum values encounter problems when opposing signals are present in a complex sample. Due to physicochemical signal reduction, cytotoxic or antagonistic effect responses, the resulting sum value is falsified. Instead, the demonstrated non-target estrogenic screening with an integrated planar chromatographic separation differentiated opposing signals, detected and prioritized important estrogenic compounds, and directly assigned tentatively the responsible compounds. Sixty pesticides were investigated, ten of which showed estrogenic effects. Exemplarily, half-maximal effective concentrations and 17β-estradiol equivalents were determined. Estrogenic pesticide responses were confirmed in six tested plant protection products. In food, such as tomato, grape, and wine, several compounds with an estrogenic effect were detected. It showed that rinsing with water was not sufficient to remove selected residues and illustrated that, though not usually performed for tomatoes, peeling would be more appropriate. Though not in the focus, reaction or breakdown products that are estrogenic were detected, underlining the great potential of non-target planar chromatographic bioassay screening for food safety and food control. Graphical Abstract

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“…The toxic effects of chlorpyrifos and its metabolites on some physiologically important enzymes ATPases, cholinesterases, and peroxidases [7] have been studied. Long-term sub-lethal exposure to this pesticide is reported to produce detrimental effects on sex hormone metabolism and nervous system functioning [8]. The binding interaction of 3,5,6-trichloro-2-pyridinol (TCP) was studied to evaluate its possibility as endocrine disruption [9], it can bind to the DNA groove making it act as a potential health hazard.…”

Section: Introductionmentioning

confidence: 99%

A validated square‐wave voltammetric method for simultaneous determination of two insecticide metabolites: 3,5,6‐trichloro‐2‐pyridinol and malathion diacid and their binding constants with human serum albumin

El‐Maali

Shafea

Mahmoud

et al. 2022

Separation Science Plus

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A proposed validated square‐wave voltammetric method and high‐performance liquid chromatography, as a comparative method, are applied to measure simultaneously two metabolites‐ of current use pesticides‐ namely 3,5,6‐trichloro‐2‐pyridinol and malathion dicarboxylic acid. Method validation and quality control parameters are studied. Method detection limits are found to be 0.043 and 0.061 μg/ml for 3,5,6‐trichloro‐2‐pyridinol and malathion dicarboxylic acid, respectively whereas the practical quantification limits are found to be 0.135 and 0.193 μg/ml (voltammetry), 0.05 and 0.1 μg/ml (high‐performance liquid chromatography) for 3,5,6‐trichloro‐2‐pyridinol and malathion dicarboxylic acid, respectively. The application of the proposed method for the analysis of water samples collected from different sources has been assessed. The interaction of these metabolites with human serum albumin was studied, a 1:1 association complex was formed and their binding constants were evaluated through the calculation to be 0.30 × 102/M for 3,5,6‐trichloro‐2‐pyridinol and 8.414 × 103/M for malathion dicarboxylic acid. The low value of the binding of 3,5,6‐trichloro‐2‐pyridinol metabolite indicates that this metabolite is more likely to be more toxic than the other metabolite.

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Endocrine disruption: molecular interactions of chlorpyrifos and its degradation products with estrogen receptor

Cited by 11 publications

References 36 publications

The pesticide chlorpyrifos promotes obesity by inhibiting diet-induced thermogenesis in brown adipose tissue

The pesticide chlorpyrifos promotes obesity by inhibiting diet-induced thermogenesis in brown adipose tissue

Non-target estrogenic screening of 60 pesticides, six plant protection products, and tomato, grape, and wine samples by planar chromatography combined with the planar yeast estrogen screen bioassay

A validated square‐wave voltammetric method for simultaneous determination of two insecticide metabolites: 3,5,6‐trichloro‐2‐pyridinol and malathion diacid and their binding constants with human serum albumin

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