Endocrine Disturbances Induced by Low-Dose Organophosphate Exposure in Male Wistar Rats

Cobilinschi, Claudia; Tincu, R.C.; Băetu, Alexandru Emil; Deaconu, C; Totan, Alexandra; Rusu, Adriana; Neagu, Paul; Grințescu, Ioana Marina

doi:10.4183/aeb.2021.177

Cited by 6 publications

(4 citation statements)

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“…Furthermore, a recent research found that the serum HDLC level can serve as a biomarker for the trunk muscle volume and function ( 48 ), making the HDLC decrement in line with the CRE decline of the present trial. In addition, the reverse responses of CORT (increase) and CHE (decrease) to TA supplementation were noticed, supported by a prior study in which CHE inhibition induced the elevation of CORT level ( 49 ). Further, the higher level of CORT in TAL could reflect an escalated degree of stress ( 50 ), and the lower GR concentration in TAH might imply the decrease of antioxidative ability ( 51 ).…”

Section: Discussionsupporting

confidence: 77%

Tannic acid reduced apparent protein digestibility and induced oxidative stress and inflammatory response without altering growth performance and ruminal microbiota diversity of Xiangdong black goats

Zuo

Liu

et al. 2022

Front. Vet. Sci.

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The present study was performed to evaluate the impacts of tannic acid (TA) supplementation at different levels on the growth performance, physiological, oxidative and immunological metrics, and ruminal microflora of Xiangdong black goats. Twenty-four goats were randomly assigned to four dietary treatments: the control (CON, basal diet), the low-dose TA group [TAL, 0.3 % of dry matter (DM)], the mid-dose TA group (TAM, 0.6 % of DM), and the high-dose TA group (TAH, 0.9 % of DM). Results showed that the growth performance was unaffected (P > 0.05) by adding TA, whilst the 0.3 % and 0.6 % TA supplementation significantly decreased (P < 0.05) the apparent digestibility of crude protein (CP) and ruminal NH3-N concentration, and raised (P < 0.05) the level of total volatile fatty acid (TVFA) in rumen. The increments of alanine aminotransferase (ALT), triglyceride (TG), cortisol (CORT), total antioxidant capacity (T-AOC), interleukin (IL)-1β, IL-6, and serumamyloid A (SAA), and decrements of globulin (GLB), immunoglobulin G (IgG), cholinesterase (CHE), glutathione reductase (GR), creatinine (CRE), growth hormone (GH), high-density lipoprotein cholesterol (HDLC), and insulin-like growth factor 1 (IGF-1) to different extents by TA addition were observed. Although the Alpha and Beta diversity of rumen bacterial community remained unchanged by supplementing TA, the relative abundance of the predominant genus Prevotella_1 was significantly enriched (P < 0.05) in TAL. It could hence be concluded that the TA supplementation in the present trial generally decreased CP digestion and caused oxidative stress and inflammatory response without influencing growth performance and ruminal microbiota diversity. More research is needed to explore the premium dosage and mechanisms of effects for TA addition in the diet of goats.

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Section: Discussionsupporting

confidence: 77%

Tannic acid reduced apparent protein digestibility and induced oxidative stress and inflammatory response without altering growth performance and ruminal microbiota diversity of Xiangdong black goats

Zuo

Liu

et al. 2022

Front. Vet. Sci.

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“…It is known as "non-thyroid impairment syndrome" and primarily manifests as a drop in triiodothyronine levels. This debilitating condition develops quickly in hazardous environments and is associated with a poor prognosis in higher vertebrates, including humans (Cobilinschi et al 2021). Considering these data, we are able to conclude that the exposure to low, environmentally realistic concentrations of malathion and terbathylazine alone and in combination was insufficient to cause significant alterations in the hypothalamic-pituitary-thyroid axis in zebrafish and provoke acute detrimental effects.…”

Section: Discussionmentioning

confidence: 80%

Multiple Molecular and Biochemical Alterations in Zebrafish Exposed to Terbuthylazine and Malathion

Khatib

Horyn

Боднар

et al. 2023

Preprint

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In the context of the global increase in pesticide applications, aquatic animals are constantly subjected to their action in natural reservoirs. Our research sought to determine the molecular and biochemical effects of an environmental exposure to commonly used chloro-s-triazine herbicide terbuthylazine and organophosphate insecticide malathion on zebrafish. To this aim, mature zebrafish were exposed to 2 and 30 µg L−1 terbuthylazine and 5 and 50 µg L−1 malathion alone and in combination for 14 days. Following exposure, induction of reactive oxygen and nitrogen species, lipid peroxidation, and protein carbonylation in the liver of treated zebrafish have been registered. Aside from the accumulation of oxidative lesions, a decrease in antioxidants and succinate dehydrogenase activity, an increase in oxidized glutathione, and enhanced apoptosis via Caspase-3 and BAX overexpression (in the case of terbuthylazine) were observed. Furthermore, terbuthylazine and malathion induced mitochondrial swelling and lactate dehydrogenase leakage in a concentrationdependent manner. Significant upregulation of ubiquitin expression and increasing cathepsin D activity were characteristics that appeared only upon terbuthylazine exposure, whereas induction of IgM was identified as the specific characteristic of malathion toxicity. Pesticides in the studied concentration didn’t provoke significant alterations in the hypothalamic-pituitary-thyroid axis in zebrafish. Co-exposure increased adverse effects of individual pesticides to zebrafish. This study should improve understanding of the mechanisms of pesticide toxicity that leads to fish impairment and biodiversity decline, as well as emphasize the importance of paying attention to pesticide traces in the environment and fish, which can have devastating effects on biota even at low concentrations.

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“…It is known as “non-thyroid impairment syndrome” and primarily manifests as a drop in triiodothyronine levels. This debilitating condition develops quickly in hazardous environments and is associated with a poor prognosis in higher vertebrates, including humans [ 72 ]. Considering these data, we are able to conclude that the exposure to low, environmentally realistic concentrations of malathion and terbuthylazine alone and in combination was insufficient to cause significant alterations in the hypothalamic-pituitary-thyroid axis in zebrafish and provoke acute detrimental effects.…”

Section: Discussionmentioning

confidence: 99%

Molecular and Biochemical Evidence of the Toxic Effects of Terbuthylazine and Malathion in Zebrafish

Khatib

Horyn

Боднар

et al. 2023

Animals

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Our research sought to determine the molecular and biochemical effects of environmentally relevant exposure to commonly used chloro-s-triazine herbicide terbuthylazine and organophosphate insecticide malathion on zebrafish. To this aim, mature zebrafish were exposed to 2 and 30 µg L−1 terbuthylazine and 5 and 50 µg L−1 malathion alone and in combination for 14 days. Aside from the accumulation of TBARS and protein carbonyls, a decrease in antioxidants and succinate dehydrogenase activity, an increase in oxidized glutathione, and enhanced apoptosis via Caspase-3 and BAX overexpression were observed. Furthermore, terbuthylazine and malathion induced mitochondrial swelling (up to 210% after single exposure and up to 470% after co-exposure) and lactate dehydrogenase leakage (up to 268% after single exposure and up to 570% after co-exposure) in a concentration-dependent manner. Significant upregulation of ubiquitin expression and increased cathepsin D activity were characteristics that appeared only upon terbuthylazine exposure, whereas the induction of IgM was identified as the specific characteristic of malathion toxicity. Meanwhile, no alterations in the zebrafish hypothalamic-pituitary-thyroid axis was observed. Co-exposure increased the adverse effects of individual pesticides on zebrafish. This study should improve the understanding of the mechanisms of pesticide toxicity that lead to fish impairment and biodiversity decline.

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Endocrine Disturbances Induced by Low-Dose Organophosphate Exposure in Male Wistar Rats

Cited by 6 publications

References 0 publications

Tannic acid reduced apparent protein digestibility and induced oxidative stress and inflammatory response without altering growth performance and ruminal microbiota diversity of Xiangdong black goats

Tannic acid reduced apparent protein digestibility and induced oxidative stress and inflammatory response without altering growth performance and ruminal microbiota diversity of Xiangdong black goats

Multiple Molecular and Biochemical Alterations in Zebrafish Exposed to Terbuthylazine and Malathion

Molecular and Biochemical Evidence of the Toxic Effects of Terbuthylazine and Malathion in Zebrafish

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