Endocytosis of latex beads by the exopinacoderm in the fresh water sponge Ephydatia fluviatilis: An in vitro and in situ study in SEM and TEM

Willenz, Ph.; Vyver, Gysèle Van de

doi:10.1016/s0022-5320(82)90005-3

Cited by 58 publications

(36 citation statements)

References 24 publications

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“…Fluorescent particles (microspheres, Duke Scientific Corporation@) with a densi.ty of 1.05 g cmT3 were used to study particle uptake by the sponges. As sponges are unable to distinguish between digestible and non-digestible particles (Pourbaix 1933, Kilian 1952, Willenz & van de Vyver 1982, a protein coating was not applied. Particles of 1, 2, 3, 6, 10 and 16 pm diameter were used.…”

Section: Flume Experimentsmentioning

confidence: 99%

Particle capture and deposition by deep-sea sponges from the Norwegian-Greenland Sea

Witte

Brattegard²,

Graf³

et al. 1997

Mar. Ecol. Prog. Ser.

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Particle uptake and deposition by the 2 most abundant deep-sea demosponge species from the Nolwegian and Greenland Sea (Thenea abyssorum) and the deep fjords of western Norway (Thenea muncata) were studied In flume expenments Fluorescent part~cles of 1, 2, 3 6 10 a n d 16 p m diameter (miclospheres, Duke S c i e n t~f~c Corporat1ono) with a density of 1 0 5 g cm were used at 2 curlent velocities, 1 5 and 5 cm s ' Both species ingested small particles exclusively ( < 6 pm and < l 0 pm, respectively) w~t h a preference for the smallest fiaction at both current speeds The results suggest that the size spectrum actually ingested depends on the s u p p l~e d particle s u e s rather than on current velocity Culrent velocity fields aiound dead speclinens were recorded and turbulence intens~t y calculated in older to determine the influence of the sponge actlng as a biogemc structure on the neai-bottom current leginle Disruption of flow conditions was detected as far as 14 cm downstream and several cm laterallv from the blogenic obstacles Bulk biodeposition rates calculated from sponge biomass and volume of Ingested particles iange between 7 and 10 mg d ' g ' ash-free dry weight K E Y WORDS Deep sea Sponge Suspension feeding Biodeposition Blogenic structure Flow regime

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Section: Flume Experimentsmentioning

confidence: 99%

Particle capture and deposition by deep-sea sponges from the Norwegian-Greenland Sea

Witte

Brattegard²,

Graf³

et al. 1997

Mar. Ecol. Prog. Ser.

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“…Similarly, even though choanocyte chambers are still present in Chondrocladia lyra -a recently described carnivorous sponge -, their choanocytes are devoid of flagella and, therefore, the sponge has a macrophagous feeding habit (Lee et al, 2012). Indeed, the ability to take up nutrients through the pinacoderm layer has been proven even in sponges equipped with the typical aquiferous system (Willenz & Van de Vyver, 1982).…”

Section: Discussionmentioning

confidence: 99%

Biological variations in a long-term ex situ cultivation: a Mediterranean demosponge as model system

et al. 2014

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The demosponge Tethya citrina Sarà & Melone was subjected to long-term aquarium farming, considering that several species belonging to this genus are a rich source of useful bioactive compounds, and in particular interesting bioactive proteins. The rearing method in the present research was mainly based on applying parameters taken from the current literature. The biological activity of the sponge was assessed in terms of survival and growth over time, status of filtering apparatus, and protein banding profiles. Farmed sponges showed a high survival rate and marked reduction in size. In addition, they were frequently affected by massive production of asexual buds. Histological and ultrastructural observations showed a gradual disorganization of the sponge choanocyte chambers, with a drastic reduction in choanocytes and a progressive increase in spherulous cells. Comparative electrophoretic analysis of the protein profiles of wild and reared specimens showed differences in protein composition and abundance between the tested groups. Such data are consistent with the increase in spherulous cells whose content was markedly electrondense, a feature stressing the proteinaceous nature of these inclusions. Experimental tank-rearing of Tethya provided promising responses in terms of high survival rate of the species and easy reproduction, though the rearing protocol used led to profound morpho-functional changes in the sponge. This confirms the inadequate state of knowledge on farming techniques for this taxon, while also highlighting potential applications in biotechnology of ex situ breeding techniques, in order to modify the sponges' biological responses.

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“…Longitudinal sections of the gemmules were made using a sharp stainless steel blade (Jakhalekar & Ghate 2013). Gemmules, both intact and sectioned, were fixed in 3% glutaraldehyde (Sigma, USA) in 0.1 M sodium cacodylate buffer (pH 7.8) for 2 hours at 4 0 C (Willenz & Van de Vyver 1982;Mukherjee et al 2015c), washed with cacodylate buffer 3 times and fixed in 1% osmium tetroxide (Sigma, USA) for 1 hour. Finally, the processed gemmules were dehydrated with graded ethanol.…”

Section: Taxonomical Identification Of Sponge Specimensmentioning

confidence: 99%

A report of hailstorm damage to two species of freshwater sponge (Demospongiae: Haplosclerida: Spongillidae) populations of West Bengal, India

Mukherjee

Ray

2016

J. Threat. Taxa

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Endocytosis of latex beads by the exopinacoderm in the fresh water sponge Ephydatia fluviatilis: An in vitro and in situ study in SEM and TEM

Cited by 58 publications

References 24 publications

Particle capture and deposition by deep-sea sponges from the Norwegian-Greenland Sea

Particle capture and deposition by deep-sea sponges from the Norwegian-Greenland Sea

Biological variations in a long-term ex situ cultivation: a Mediterranean demosponge as model system

A report of hailstorm damage to two species of freshwater sponge (Demospongiae: Haplosclerida: Spongillidae) populations of West Bengal, India

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