Endogenous Cu and Zn nanocluster-regulated soy protein isolate films: excellent hydrophobicity and flexibility

Kuang, Li; Chen, Hui; Li, Ying; Li, Jianzhang; He, Jing

doi:10.1039/c5ra09231k

Cited by 37 publications

(23 citation statements)

References 41 publications

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“…The UV absorption of lysozyme at 280 nm (native lysozyme) was enhanced and shifted to 290 nm once the lysozyme, Tollen's reagent, and d ‐glucose solution were mixed, which indicated that more hydrophobic residues were exposed in the aqueous environment due to the protein unfolding (Figure b) . After a plateau from 0 to 15 min, an obvious second redshift of the absorption peak from 290 to 297 nm was observed during the reaction time from 15 to 45 min, which reflected that the surface hydrophobicity of the protein was further increased (Figure b) …”

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confidence: 98%

Protein‐Bound Freestanding 2D Metal Film for Stealth Information Transmission

et al. 2018

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PEI)-assisted crosslinking with catechol moieties, which could further be used as a Janus platform to support the reduction and attachment of silver nanoparticles on the film surface. [7] Freestanding organicmetal 2D films have potential in many application fields, such as in sensing, surface-enhanced Raman scattering, catalytic reaction, near-infrared photothermal therapy, and biomedical areas. [8][9][10][11] Unfortunately, such films are fabricated via multiple, time-consuming steps; [11,12] in addition, the sacrifice of metal conductivity greatly limits the applications of these materials in the electrical device field. [2,13] Herein, we report a facile, environmentally friendly and bio-based redox system to merge metal nanoparticles under ambient conditions in an aqueous solution via protein bonding, which is distinctive from traditional welding of nanomaterials at high temperatures/ pressures. We discover that the silver nanoparticles from the in situ reduction of silver ammonium ions by glucose were bound by ultrathin amyloid-like β-sheet stacking of lysozyme to create a freestanding large-area (e.g., 400 cm 2 ) 2D silver film at the air/water interface with a purity up to 98%. We prove the great ability of this reaction system toward controlled synthesis of highly reflective and highly conductive silver films with elongation nearly 10 times higher than that of pure metal without protein bonding. These characteristics allow the protein-bound silver films to crucially participate in realistic applications, such as in strain/pressure sensors and artificial throats with ultrasensitive capability for stealth transmission of Morse code via the detection of minute finger tapping and for silent speech recording via the detection of tiny vibrations of the human throat, a result never reported before. No special equipment is necessary for this one-step method, and we further demonstrate that the bonding function of lysozyme is general to other proteins (e.g., albumin, α-amylase, collagen, keratin, and pepsin) and other metal films besides Ag (e.g., Au and Cu) are synthesized easily by this strategy.The protein assembler lysozyme, generally recognized as a safe material by the US Food and Drug Administration, is commercially available at low cost from egg white, body fluids of animals and plant cells. [14] Figure 1a schematically illustrates the synthetic procedures of a lysozyme-bound silver film. Lysozyme from egg white, Tollen's reagent, and d-glucoseThe welding and sintering of nanomaterials is usually achieved at high temperatures and high pressures. Here, it is found that merging of metal nanoparticles occurs under ambient conditions in an aqueous solution via protein bonding. It is discovered that the silver nanoparticles from the in situ reduction of silver ammonium ions by glucose undergo confined nucleation and growth and are bound by ultrathin amyloid-like β-sheet stacking of lysozyme. This merging of silver nanoparticles creates a freestanding large-area (e.g., 400 cm 2 ) 2D silver film at the air/water i...

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Protein‐Bound Freestanding 2D Metal Film for Stealth Information Transmission

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“…The addition of zinc ions reduced the brittleness with almost no holes and cracks that could be seen for formulation 2, 3, 4, 5, and 6 ( Figure 6a). This suggested that the interactions between soybean meal, blood meal, and Zn had a plasticizing effect on protein matrix and improved the toughness of the material [43]. It was noticed that when the content of zinc ions increased to 2%, large and wide cracks appeared on the film (Figure 6a, formulation 7), which was due to the excessive metal ions leading to protein precipitation and salting out.…”

Section: Performances Of the Soybean Meal And Blood Meal-based Adhesivementioning

confidence: 98%

A Tough and Mildew-Proof Soybean-Based Adhesive Inspired by Mussel and Algae

Bai

Liu

2020

Polymers

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Despite the recent advances in protein-based adhesives, achieving strong adhesion and mold resistance in wet environment is challenging. Herein, a facile fabrication technology of preparing tough bio-adhesive by incorporating soybean meal and blood meal is presented. Inspired by the marine mussel byssi and brown algae, metal coordination was introduced into a loosely bound protein system to construct multiple chemical cross-linking networks. Mixed alkali-modified blood meal (mBM) was mixed with soybean meal, then 1,6-hexane dioldiglycidyl ether (HDE) and zinc ion were introduced to fabricate soybean meal and blood meal-based adhesives. The attained adhesives exhibited good thermal stability, water resistance (the wet shear strength is 1.1 MPa), and mold resistance, with appropriate solid content (34.3%) and relatively low moisture uptake (11.9%). These outstanding performances would be attributed to the reaction of 1,6-hexane dioldiglycidyl ether with protein to form a preliminary cross-linking network; subsequently, the coordination of zinc ions with amino or carboxyl strengthened and toughened the adhesive. Finally, the calcium ions gelled the adhesives, providing cohesion force and making the network structure more compact. This study realized the value-added utilization of protein co-products and developed a new eco-friendly bio-based adhesive.

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“…Cu and Zn nanocluster as well as silicon oxides nanoparticles have been incorporated in SPI matrices to get the reinforced SPI material with improved properties [5,6]. Carbon nanotubes (CNTs) [7], chitin whiskers [8], layered silicate [9][10][11], carbon nanoparticles [12] and silica nanoparticles [13] have been explored as reinforcement materials for SPI.…”

Section: Introductionmentioning

confidence: 99%

ZnSe Nanoparticles Reinforced Biopolymeric Soy Protein Isolate Film

Kumar

Praveen²,

Rani³

et al. 2019

Journal of Renewable Materials

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ZnSe nanoparticles have been synthesized by microwave assisted method by using zinc chloride, selenium powder and ethylene diamine. The synthesized nanoparticles have been characterized structurally by FT-IR and XRD as well as morphological characterization was done by scanning electron microscope (SEM). The crystallite size after synthesis was obtained around 30 nm for pure ZnSe nanocrystallites. However, from SEM micrograph, agglomerated ZnSe nanoparticles of irregular shapes were observed. The assynthesized ZnSe nanoparticles at different contents (1 to 5% w/w w.r.t SPI) were incorporated into soy protein isolate (SPI) to produce reinforced SPI films by solution casting method. The ZnSe nanoparticles incorporated SPI suspensions were subjected to molecular mass and specific conductivity studies. Neat and ZnSe nanoparticles incorporated SPI films were structurally and mechanically characterized by FT-IR and tensile properties, respectively. Transmittance and water uptake studies were also carried out for ZnSe nanoparticles incorporated SPI films. The tensile strength and modulus increased from 5.80 MPa to 10.06 MPa and 18.84 MPa to 94.70 MPa with the increase in the contents of ZnSe nanoparticles from 0 to 5%. Moreover, the results also revealed a good antibacterial effect in ZnSe nanoparticles incorporated SPI film. The main application of nanoparticles incorporated SPI film will be in the area of biodegradable packaging.

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Endogenous Cu and Zn nanocluster-regulated soy protein isolate films: excellent hydrophobicity and flexibility

Cited by 37 publications

References 41 publications

Protein‐Bound Freestanding 2D Metal Film for Stealth Information Transmission

Protein‐Bound Freestanding 2D Metal Film for Stealth Information Transmission

A Tough and Mildew-Proof Soybean-Based Adhesive Inspired by Mussel and Algae

ZnSe Nanoparticles Reinforced Biopolymeric Soy Protein Isolate Film

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