Endogenous dipeptide‐carnosine supplementation ameliorates hypobaric hypoxia‐induced skeletal muscle loss via attenuating endoplasmic reticulum stress response and maintaining proteostasis

Agrawal, Akanksha; Rathor, Richa; Kumar, Ravi Shankar; Singh, Som Nath; Kumar, Bhuvnesh; Suryakumar, Geetha

doi:10.1002/iub.2539

Cited by 9 publications

(3 citation statements)

References 71 publications

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“…In recent years, nutritional strategies have emerged and it has been well demonstrated that they can increase the level of production and improve the health of animals and products obtained from them [16], as well as to enhance livestock productivity [17,18]. There is evidence that L-carnosine supplementation ameliorates skeletal muscle protein loss mainly by maintaining proteostasis redox homeostasis in skeletal muscles [19]. In addition, L-carnosine may be beneficial in maintaining higher metabolic activity of muscle fibers [20], both under in vivo [21,22] and in vitro conditions [23].…”

Section: Introductionmentioning

confidence: 99%

Improved Satellite Cell Proliferation Induced by L-Carnosine Benefits Muscle Growth of Pigs in Part through Activation of the Akt/mTOR/S6K Signaling Pathway

et al. 2022

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(1) Background: L-carnosine (β-alanyl-L-histidine), a natural dipeptide, exists at relatively high concentrations in skeletal muscles, and has been shown to protect cells from adverse conditions due to its antioxidant, anti-aging, anti-glycation, and buffering properties. Satellite cells (SCs), residing on the myofiber surface, are crucial for muscle post-growth and regeneration. However, the effects of L-carnosine on muscle development of pigs in vivo, on proliferation and growth of SCs in vitro, and the relationship between SCs and muscle development have not yet been investigated. (2) Methods: The objective of this study was to assess the effect of dietary L-carnosine on growth performance and longissimus dorsi muscle development of pigs in vivo, and to elaborate its molecular mechanisms in vitro using L-carnosine-treated SCs. (3) Results: It was shown that L-carnosine supplementation (0.2 and 2 mM) increased (p < 0.05) SC proliferation and cell percentage in the synthesis (S) phase and decreased cell percentage in the resting (G0)/first gap (G1)/phase. Moreover, average daily gain (ADG) of pigs fed diets containing 0.1% of L-carnosine was higher (p < 0.05) than that of pigs fed diets without L-carnosine, and the longissimus dorsi muscle weight of pigs assigned to the L-carnosine treatments was 7.95% higher compared to control pigs. Both in the longissimus dorsi muscle and cultured SCs of pigs, the Akt/mTOR/S6K signaling pathway was activated (p < 0.05), suggesting that L-carnosine improved muscle growth and SC proliferation of pigs. (4) Conclusions: Considering the important role of SCs in post-natal muscle growth, there is evidence that L-carnosine may improve muscle growth of pigs through promoting SC proliferation via activating the Akt/mTOR/S6K signaling pathway.

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Section: Introductionmentioning

confidence: 99%

Improved Satellite Cell Proliferation Induced by L-Carnosine Benefits Muscle Growth of Pigs in Part through Activation of the Akt/mTOR/S6K Signaling Pathway

et al. 2022

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“…The effect of hypoxia on muscle mass and function is less investigated compared to inactivity. Several studies have reported that prolonged exposure to hypoxia (in response to various pathological and physiological conditions including high altitude) results in loss of muscle mass, structure, and performance [ 4 , 5 , 6 , 7 ]. Moreover, hypoxia aggravates inactivity-related muscle wasting [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Short-Term Mild Hypoxia Modulates Na,K-ATPase to Maintain Membrane Electrogenesis in Rat Skeletal Muscle

Кравцова

Fedorova

Tishkova

et al. 2022

IJMS

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The Na,K-ATPase plays an important role in adaptation to hypoxia. Prolonged hypoxia results in loss of skeletal muscle mass, structure, and performance. However, hypoxic preconditioning is known to protect against a variety of functional impairments. In this study, we tested the possibility of mild hypoxia to modulate the Na,K-ATPase and to improve skeletal muscle electrogenesis. The rats were subjected to simulated high-altitude (3000 m above sea level) hypobaric hypoxia (HH) for 3 h using a hypobaric chamber. Isolated diaphragm and soleus muscles were tested. In the diaphragm muscle, HH increased the α2 Na,K-ATPase isozyme electrogenic activity and stably hyperpolarized the extrajunctional membrane for 24 h. These changes were accompanied by a steady increase in the production of thiobarbituric acid reactive substances as well as a decrease in the serum level of endogenous ouabain, a specific ligand of the Na,K-ATPase. HH also increased the α2 Na,K-ATPase membrane abundance without changing its total protein content; the plasma membrane lipid-ordered phase did not change. In the soleus muscle, HH protected against disuse (hindlimb suspension) induced sarcolemmal depolarization. Considering that the Na,K-ATPase is critical for maintaining skeletal muscle electrogenesis and performance, these findings may have implications for countermeasures in disuse-induced pathology and hypoxic therapy.

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“…Carnosine (Carn) is a histidine dipeptide that is naturally occurring and endogenously generated in human body, carnosine is broadly distributed in the kidney, brain, muscle, stomach and in significant proportions, skeletal muscle [ 28 ]. Numerous biological actions of this dipeptide have been demonstrated, including the capacity to chelate metal ions, anti-oxidant activity, suppressing protein glycosylation, anti-inflammatory impact and anti-senescence properties.…”

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confidence: 99%

Impact of HIF1-α/TGF-β/Smad-2/Bax/Bcl2 Pathways on Cobalt chloride-induced Cardiac and Hepatorenal Dysfunction

Kadry¹,

Ali²

2023

Future Sci. OA

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Background: Cobalt chloride (CoCl2) is a ferromagnetic ubiquitous trace element extensively dispersed in the environment. Nevertheless, it may merit human hazard. Aim: Excess cobalt can harm vital organs this paves the way to elucidate the toxic impact of CoCl2 on the liver, kidney and heart. Method: CoCl2 was injected in a dose of (60 mg/kg, S.C.) proceeded via Carnosine (200 mg/kg) and/or Arginine (200 mg/kg) treatment 1 month, 24 and 1 h, prior to CoCl2-intoxication. Results: CoCl2 significantly alleviated hemoglobin concentration and BCl2; meanwhile, protein expression of transforming growth factor (TGF-β), hypoxia-inducible factor (HIF-1α), Mothers against decapentaplegic (Smad-2), AKT protein expression and Bax/Bcl2 ratio was noticeably elevated. Conclusion: The combination of the aforementioned antioxidants exerted a synergistic anti-apoptotic impact in all target tissues.

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Endogenous dipeptide‐carnosine supplementation ameliorates hypobaric hypoxia‐induced skeletal muscle loss via attenuating endoplasmic reticulum stress response and maintaining proteostasis

Cited by 9 publications

References 71 publications

Improved Satellite Cell Proliferation Induced by L-Carnosine Benefits Muscle Growth of Pigs in Part through Activation of the Akt/mTOR/S6K Signaling Pathway

Improved Satellite Cell Proliferation Induced by L-Carnosine Benefits Muscle Growth of Pigs in Part through Activation of the Akt/mTOR/S6K Signaling Pathway

Short-Term Mild Hypoxia Modulates Na,K-ATPase to Maintain Membrane Electrogenesis in Rat Skeletal Muscle

Impact of HIF1-α/TGF-β/Smad-2/Bax/Bcl2 Pathways on Cobalt chloride-induced Cardiac and Hepatorenal Dysfunction

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