Endogenous levels of abscisic acid and indole-3-acetic acid during in vitro rooting of Wild Cherry explants produced by micropropagation

Label, Ph.; Sotta, Bruno; Miginiac, Émile

doi:10.1007/bf00024663

Cited by 29 publications

(13 citation statements)

References 14 publications

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“…Insufficient information is available to fully comprehend these variations in ABA content. In cherry microcuttings, a decrease in ABA content was observed during the early stages of root formation and this decline was concomittant to an increase in ABA levels in the apical part of the shoots [9]. The results presented here support the view [ 151 that ABA may be one of the factors involved in the inhibition of root formation in difficult-to-root material.…”

Section: Discussionsupporting

confidence: 79%

Endogenous indole-3-acetic acid and abscisic acid in apple microcuttings in relation to adventitious root formation

1992

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The effects of applying indole-3-butyric acid (IBA) for periods up to 48 h were examined in difficult-to-root microcuttings (from newly-established cultures) and in easy-to-root microcuttings (from long-term subcultures) of 'Jonathan' apple (Ma/us X domestica Borkh). In easy-to-root material, 20% of the microcuttings produced roots in the absence of IBA, while 6 h exposure to 10 PM IBA gave 100% rooting of microcuttings. In contrast, root formation in difficult-to-root material was IBA-dependent. Maximum rooting of these microcuttings (50%) required 24 h exposure to IOpM IBA.Variation in the endogenous levels of free indole-3-acetic acid (IAA) during the course of root induction was similar in microcuttings of both types but there were marked differences in endogenous abscisic acid (ABA) levels. In easy-to-root microcuttings ABA remained at a constant low level, but in difficult-to-root material ABA exhibited marked fluctuations and was present at higher concentrations than in easy-to-root microcuttings.

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Section: Discussionsupporting

confidence: 79%

Endogenous indole-3-acetic acid and abscisic acid in apple microcuttings in relation to adventitious root formation

1992

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“…At the beginning of culture on the RIM, an IAA peak was observed . The importance of IAA accumulation for root induction has already been reported for IBA-treated explants of Prunus avium [16] . In the present work too, the IAA detected in microcuttings during root induction was probably exogenous coming from the IBA which was applied in the RIM .…”

Section: Discussionmentioning

confidence: 98%

Relations between auxin and cytokinin contents and in vitro rooting of tree Peony (Paeonia suffruticosa Andr.)

et al. 1994

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In this work, a combined HPLC-ELISA technique was used to associate in vitro rooting capacity of tree peony microcuttings with contents of cytokinin and auxin ; the cytokinin mainly detected corresponded to the N 6 -benzyladenine which had been added to the multiplication medium . Rooting capacity of explants was favoured by a preliminary accumulation of endogenous IAA only when levels of the BA absorbed from the multiplication medium had decreased . Main shoots coming from a 5-weeks subculture fulfilled these hormonal conditions and were the best microcuttings for rooting (87% rooting) . Main shoots coming from shorter cycles or axillary shoots coming from a 5-weeks cycle always contained high benzyladenine levels and had a low rooting capacity (25-55% rooting) . Root induction was associated with an early peak of indole-3-acetic acid followed by a 10-fold lower peak of endogenous ribofuranosyl-isopentenyladenine . Only a low and transitory accumulation of isopentenyladenine occurred during root development, and this could explain the lack of shoot development . Root development was efficient, especially in a medium containing activated charcoal, which led to an almost 3-fold decrease of IAA contents in roots .Abbreviations : AC = activated charcoal ; BA = N6 -benzyladenine ; ELISA = enzyme linked immunosorbent assay ; HPLC = high performance liquid chromatography ; IAA = indole-3-acetic acid ; IBA = indole-3-butyric acid ; iP = N6 -(62-isopentenyl)adenine; RDM = root development medium ; RIM = root induction medium ; 9RIP = 9-,0-d-ribofuranosyl-iP ; 9RZ = 9-a-d ribofuranosyl-zeatin ; Z = zeatin .

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“…CKs affect the reactivity and growth of buds in many species, either directly (Von Arnold and Tillberg, 1987;Label et al, 1988;Pilate et al, 1989;Young, 1989) or via root activity related to juvenility (Franclet, 1981). CKs induce apex rejuvenation in mature trees of the species Pseudotsuga menziesii (Bakkaoui, 1986) and Picea abies (Tsogas and Bouriquet, 1983), characterized by reactivation.…”

Section: Mineral Elementsmentioning

confidence: 99%

Maturation of woody plants: a review of metabolic and genomic aspects

et al. 1991

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Endogenous levels of abscisic acid and indole-3-acetic acid during in vitro rooting of Wild Cherry explants produced by micropropagation

Cited by 29 publications

References 14 publications

Endogenous indole-3-acetic acid and abscisic acid in apple microcuttings in relation to adventitious root formation

Endogenous indole-3-acetic acid and abscisic acid in apple microcuttings in relation to adventitious root formation

Relations between auxin and cytokinin contents and in vitro rooting of tree Peony (Paeonia suffruticosa Andr.)

Maturation of woody plants: a review of metabolic and genomic aspects

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